超高效液相色谱-四极杆飞行时间质谱法快速筛查睫毛用化妆品中前列腺素F2α类化合物

采用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF MS/MS)技术,建立了睫毛用化妆品中9种前列腺素F2α类化合物(比马前列素、曲伏前列素、他氟前列素、他氟乙酰胺、拉坦前列素、比马前列素酸甲基酯、氯前列醇异丙基酯、15-酮-曲伏前列素、比马酰胺)的快速定性筛查方法。样品经乙腈超声提取,采用Waters ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,以2 mmol/L甲酸铵水溶液(含0.05%甲酸)-乙腈为流动相,分离后进入四极杆飞行时间质谱,采用电喷雾离子源正离子模式(ESI+),以MSE模式进行数据采集,建立了9种前列腺素F2α类化合物的筛查数据库。9种化合物在各自质量浓度范围内线性关系良好,相关系数(r²)均大于0.99,检出限为0.075～0.75μg/g,定量下限为0.25～2.5μg/g。所建立的筛查数据库无需对照品即可对化妆品中的9种前列腺素F2α类化合物进行筛查。结果表明,该方法特异性良好,应用于48批睫毛类化妆品的定性筛查具有良好的选择性,可为化妆品中前列腺素F2α类化合物的筛查分析提供技术支...     

Determination of perfluorooctane sulfonic acid in cosmetics by high-perfor⁃ mance liquid chromatography-tandem mass spectrometryEI北大核心CSCD

A high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）method was developed for the determination of perfluorooctane sulfonic acid （PFOS）in cosmetics. The cosmetic samples were extracted with methanol by ultrasonication,and PFOS was quantified by HPLC-MS/MS. The samples were detected in negative multiple reaction monitoring（MRM）mode using an Agilent Poroshell 120 EC-C18 （100 mm× 2.1 mm,2.7 µμm）column with a gradient elution using 0.1% formic acid aqueous solution（A）-methanol（B）as the mobile phase. The results showed that the linearity of PFOS was good in the range of 1-100 µμg/L with the correlation coefficient r2 larger than 0.9998;the limits of detection and limits of quantification were 0.015 and 0.050 mg/kg,respectively. The average recoveries were 98.7%-112.5%,and the relative standard deviations （RSDs）were less than 5%. The method is suitable for the determination of PFOS in cosmetics. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

高效液相色谱法同时测定化妆品中辣椒碱等9种热感剂

建立了测定化妆品中辣椒碱等9种热感剂的高效液相色谱法。样品经80%甲醇超声提取后,过滤,以乙腈-0.5%甲酸水溶液为流动相,采用SymmetryShieldTMRP18色谱柱（250 mm × 4.6 mm,5 μm）分离,液相色谱分离,二极管阵列检测器进行检测。在优化实验条件下,9种热感剂的分离度好,专属性高,其质量浓度在0.2 ~ 100 μg/mL范围内与峰面积呈良好线性关系,相关系数（r2）均不小于0.999 5,检出限（LOD）为0.001 0 ~ 0.003 4 mg/kg。以水基类、乳剂类、膏霜类3种阴性样品作为空白基质,9种热感剂在0.004、0.02、1 mg/kg 3种加标水平下的回收率为75.6% ~ 115%,相对标准偏差（RSD）为0.083% ~ 7.0%。该方法专属性强、灵敏度高、准确度好,可用于化妆品中9种热感剂含量的测定。