海洋走航式单边连续观测地震折射波Δt解释方法--1种新的浅层折射单支时距曲线解释方法

在浅层工程折射地震测量中 ,通常采用相遇观测系统采集数据和相应的解释方法进行解释 ;对于单边观测采集方法由于与其相应的解释方法较少而少被采用。本文提出 1种方法简单、精度可靠、可直接利用同一点相邻 2排列 2次观测的初至折射时间并适合于海洋走航式单边连续观测系统的折射初至资料解释方法。其精度基本与同观测段的采用相遇观测的差异时距方法解释的精度十分接近。该方法即适合于人工解释又适合于计算机自动解释。     

沪蓉高速柴家湾隧道围岩稳定性分析及支护效果研究

隧道围岩的应力分布及变形特征对保证其长期稳定性具有关键的作用。本文以柴家湾隧道为例进行实态建模,通过采用有限元软件ANSYS对其施工全过程进行了三维弹塑性数值模拟计算。研究结果表明,隧道拱顶和拱脚处应力集中现象明显,局部可能出现拉应力;变形主要集中在拱顶、拱腰部位,衬砌单元所受内力在规定强度范围以内,支护措施合理有效。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

恶劣环境下GPS/BDS混合双差模糊度固定性能分析

针对在恶劣观测环境下每个GNSS系统只观测到较少的几颗卫星,采用标准双差模型很难实现整周模糊度固定的问题,该文对GPS/BDS系统间混合双差模型进行研究,利用该模型计算出GPS/BDS系统偏差。在此基础上,利用实验分别对模拟和真实恶劣观测环境下的模糊度固定性能进行分析,实验结果表明,恶劣环境下GPS/BDS系统间混合双差模型表现良好,相较于经典的双差模型可将模糊度固定平均所需时间分别缩短43%和33%,模糊度固定成功率分别提高107%和31%。     

Effects of Seawater Acidification on Early Development of Clam Cyclina sinensis

Anthropogenic emission of atmospheric carbon dioxide(CO_2) has led to a rapid increase in atmospheric CO_2 concentration. Increasing atmospheric CO_2 can reduce seawater pH and carbonate ions, which may adversely affect the survival of the larvae of calcareous animals. Cyclina sinensis is a commercially and ecologically important species in several Asian countries. Living in coast shallow waters, this species has experienced the coastal environmental changes frequently throughout its life cycle. In this study, we simulated possible future seawater pH values including 8.2, 7.8 and 7.4 and examined the effects of ocean acidification on the early development of C. sinensis. Clam embryos were incubated for 48 h(2 d) in control and high-CO_2 seawater to compare embryogenesis, larval growth and swimming behavior. Fertilization rate was quite sensitive to pH, and moderate acidification could induce a significant decrease in fertilization rate. However, only extreme acidification could bring significant negative effect to hatching rate, body size, and average path velocity of trochophora. Moreover, with seawater acidification, C. sinensis needs much more time to reach the same developmental stage, which increases the risk of larva survival. Together with recent studies demonstrating negative impacts of high CO_2 on fertilization and larva swimming behavior, the results imply a future decrease of C. sinensis populations in oceans if its acclimation to the predicted environmental alteration does not occur.     

水下光学无线通信的海水信道特性研究

提出了将具有比声学高达几倍的数据通信速率,良好的安全性和隐蔽性的光学无线通信技术应用于海军、海洋科学研究和水下工程等领域,实现高速率的水下无线通信技术实现海量数据的信息交换.基于生物光学特性的水下光学信道模型,建立了水下光学通信系统性能分析方法.并对基于发光二极管(LED)的水下光学无线通信系统进行了仿真,其结果表明所建立的方法可以进行各种海域水质环境的模拟,便于时水下光学无线通信系统性能进行预测评估,为水下光学无线通信系统的设计方案的评估提供了依据.     

红藻碳代谢的研究进展

红藻门(Rhodophyta)在全世界约有760属,4 410种,绝大多数海产,少数生于淡水,分布于各地,包括极地.中国已知有127属,300种,分布于南北各海区,淡水种类极少.     

个旧高松矿区三维地质建模及储量估算

文章以云南个旧东矿区高松矿田钻孔数据和勘探线地质剖面图为基础,结合中段地质平面图,以控矿条件定性分析和控矿作用定量化为导向,基于Micromine软件平台建立研究区地形、地层、断裂以及矿体的三维模型,提取地质控矿作用定量化指标,对矿区矿化分布进行不同方法的品位插值,获得已知矿体的储量计算结果.