胃癌血清肿瘤标志物的检测及其意义的研究

目的：探讨血清肿瘤标志物与胃癌腹膜转移的关系．方法：收集35例胃癌伴腹膜转移患者血清标本并检测与胃癌相关的肿瘤标志物的水平．结果：CEA、CA19—9、CA-125及CA72—4水平均有不同程度的升高,CA15—3在正常范围内．结论：联合检测CEA、CA19—9、CA-125及CA72—4有助于提高胃癌及腹膜转移的检测率,而CA15—3在胃癌及腹膜转移中无指示作用．     

人肿瘤细胞悬液裸鼠皮下接种及影响移植成功的因素

根据文献报道及作者实验中的体会 ,对人肿瘤细胞悬液裸鼠皮下接种方法及影响移植成功的因素从瘤细胞、裸鼠和操作者等三方面进行综述。较好地认知人肿瘤细胞悬液裸鼠皮下接种的具体操作程序和影响移植是否成功的多方面因素 ,为人肿瘤裸鼠皮下移植模型成功建立提供参考     

彩色多普勒超声诊断甲状腺肿瘤386例分析

甲状腺肿瘤的超声图像特征与病理组织之间存在事实上的相关性．笔者回顾性分析超声拟诊的386例甲状腺肿瘤患者的超声诊断资料，经与手术病理对照，从肿瘤的部位、大小、声像图特征进行分析，评价超声显像对甲状腺良恶性肿瘤的诊断价值．     

一例家族性多发肿瘤遗传的调查报告

介绍一个肿瘤多发家系．该家系4代22人，8名癌症患者，占36％．8名患者中5人为多发性肿瘤，占62．5％，肝癌患者4人，占50%，提示该家系肿瘤具有家族聚集现象，呈常染色体显性遗传．     

带有时滞项的肿瘤生长模型的数学分析

本文研究三个带有时滞项的描述肿瘤生长的数学模型。时滞大小为细胞从开始分裂到新个体的形成所需要的时间。我们假设肿瘤生长没有抑制物作用，研究了问题解的存在唯一性及其初步性质和t→∞时解的渐近状态，证明了σ^-〈σ∞当时，未血管化的肿瘤体积不会无限制的增大或消失，且唯一存在的稳态解是一致渐近稳定的；当σ≥σ∞时，未血管化的肿瘤体积不会无限制的增大，但有可能消失。     

Efficacy of tumor cell vaccine after incorporating monophosphoryl lipid A (MPL) in tumor cell membranes containing tumor-associated ganglioside

Murine B16 melanoma expresses the ganglioside. GM₃. GM₃ shed from tumor cells is immunosuppressive and promotes tumor growth¹. Reduction or elimination of the shed GM₃ could be therapeutic, and the anti-GM₃ antibodies may reduce and clear the shed ganglioside. To test this hypothesis, mice were challenged with tumor cells, with or without inducing anti-GM₃ antibody response. Since gangliosides are poor immunogens and T-cell independent antigens, an adjuvant (monophosphoryl lipid A (MPL), a non-toxic lipid A ofSalmonella), directed against B-cells, was employed. MPL was incorporated onto liposomes and into the surface membrane of B16 mouse melanoma cells; both are rich in GM₃. C57BL/6J mice immunized with MPL-liposomes or MPL-B16 cells responded with elevated levels of anti-GM₃ IgM. Non-immunized mice or mice immunized with B16 cells alone or ganglioside GM₃ alone (without MPL) elicited poor anti-GM₃ IgM response, confirming the GM₃'s immunologic crypticity and MPL's immunopotentiating effect. MPL's immunopotentiating effect was improved by coupling it to melanoma cell membranes C57BL/6J mice were immunized with irradiated B16 alone or MPL alone or MPL-conjugated irradiated B16. After three weekly immunizations, each mouse received a challenge dose of viable syngeneic B16. Neither MPL alone nor B16 alone had a significant effect on tumor growth or host survival; however, administration of MPL-conjugated B16 cells significantly prevented tumor growth and prolonged survival. Our results indicate that MPL-incorporated B16 cells augment the anti-GM₃ IgM response, which may reverse GM₃-induced immunosuppression by eliminating tumor-derived GM₃, and restore immunocompetence.     

嵌合抗原受体T细胞疗法专利态势分析

嵌合抗原受体T细胞疗法是最有可能战胜癌症的肿瘤免疫疗法,是生命科学领域极具发展前景的前沿产业,对知识产权的保护至关重要。本文对嵌合抗原受体T细胞疗法的国际和国内专利态势进行分析,包括对专利申请趋势、专利产出国、专利应用国、主要申请人、技术聚类主题等进行分析,从而比较我国与国际的差距与不足,有助于我国嵌合抗原受体疗法行业更好地运用专利手段保护自身知识产权,促进企业创新发展。本文研究发现,相比于美国,我国科研院所在嵌合抗原受体T细胞疗法领域的专利产出不高,反应了我国在该技术基础研究投入方面的不足,我国对全球范围内的专利布局也缺乏重视。建议我国应加大对嵌合抗原受体T细胞疗法的基础研发投入,开发和掌握关键技术;注重国际专利布局,增强国际市场竞争力;强化科研院所和企业之间的联动合作,以鼓励和促进专利技术的转化。     

Indocyanine green-based nanodrugs: A portfolio strategy for precision medicine

As the sole cyanine dye approved by the Food and Drug Administration (FDA), indocyanine green (ICG) has been widely applied in solid tumor and metastases surgical navigation as well as malignant tissue destruction. The great performance of ICG benefits from excellent fluorescent properties in the near infrared region (NIR) and reactive oxygen species (ROS) or hyperthermia effects under irradiation. Nevertheless, some intrinsic limitations, such as unstable optical properties, concentration-dependent aggregation, proneness to photo-bleaching, and poor aqueous stability in vivo, severely restrict its further applications. To overcome these challenges, ICG-based nanodrugs (nano-ICG), as one kind of portfolio strategy, could bridge the efficient cargo-carriers and ICG molecules to artificially improve their existing performance. In this mini-review, we will focus on the developed engineering strategies for nano-ICG formulation synthesis and their desired performance in tumor tracing and eradication. A critical analysis of assembly mechanisms, excellent performance, and further improvement tactics will be examined.     

癌性神经病1例临床报告

恶性肿瘤严重威胁着人类健康,恶性肿瘤能引起神经系统损伤,部分恶性肿瘤病人神经系统损伤出现在癌肿被发现之前,恶性肿瘤颅内转移是癌肿神经系统损伤中最常见的一种,约占颅内肿瘤的6%～10%.