甘氨酸对坏死性肠炎鼠血清IL-1和IL-6水平的影响(英文)

目的：探讨甘氨酸对内毒素和缺氧诱导的坏死性肠炎(NEC)鼠血清炎性因子IL-1与IL-6的作用。方法：40只SD大鼠随机分为甘氨酸+LPS组和NS+LPS对照组。甘氨酸组大鼠静脉给予甘氨酸1 g/kg,5min后给予内毒素2 mg/kg,NS对照组用等量的生理盐水代替甘氨酸,内毒素剂量同前。所有大鼠注射LPS 90min后氧吸入浓度从21％降至5％,继续机械通气至鼠死亡或存活180 min,实验结束时采血样和小肠标本。用双抗夹心ELISA法测定血清IL-1与IL-6的含量,肠组织做病理检查并进行NEC分度。结果：甘氨酸组的存活时间(159.25±22.78)min长于NS对照组(138.75±19.05)min,差异有显著性(P<0.01)。甘氨酸组小肠病理损伤程度明显明显低对照组(P<0.01)。甘氨酸组血清IL-1的含量为(149.1±76.1)ng/L,显著低于对照组(472.1±505.6)ng/L(P<0.01);血清IL-6的含量为(204.8±163.5)ng/L,亦显著低于对照组(585.8±574.5)ng/L(P<0.01)。结论：甘氨酸可降低内毒素和缺氧诱导的坏死性肠炎(NEC)鼠血清IL-1和IL-6含量水平,减轻肠病理损伤。     

Difficulties in assessing the effect of strychnine on the outcome of non-ketotic hyperglycinaemia. Observations on sisters with a mild T-protein defect

Sisters with a mild variant of non-ketotic hyperglycinaemia resulting from a defect in the T-protein of the glycine cleavage system had different clinical outcomes. The older sister was ascertained at 6 months of age because of mental retardation. She received only brief treatment with sodium benzoate from 11–15 months and at 15 years of age is profoundly retarded and has epilepsy. The younger sister was diagnosed 36h after birth, was treated with strychnine, sodium benzoate and arginine from the neonatal period and at 27 months of age is only moderately retarded and free of seizures. The possible role of strychnine in the improved outcome is discussed.Abbreviations NKH non-ketotic hyperglycinaemia - CSF cerebrospinal fluid     

甘氨酸和 Triton X-100 对 ZZ-EGFP 融合蛋白分泌表达影响的初步研究

 目的 考察不同浓度的甘氨酸和 Triton X-100 对葡萄球菌蛋白质 A ZZ 亲和肽-增强型绿色荧光蛋白（ZZ-EGFP）融合蛋白在大肠杆菌分泌表达的影响。 方法 研究设计为两因素三水平析因设计。向液体培养基中分别加入终浓度 0、1%、2% 的甘氨酸和 0、1%、2% 的 Triton X-100（共 9 种组合方式，终浓度均为 0 者为对照组），诱导大肠杆菌周质腔内 ZZ-EGFP 融合蛋白泄漏到液体培养基中，以培养上清液荧光强度为观察指标，通过 ZZ- EGFP 融合蛋白浓度-荧光强度标准曲线快速检测培养基中 ZZ-EGFP 融合蛋白表达量。 结果 培养基中分别加入 1%、2% 甘氨酸或 1%、2% Triton X-100 培养后，培养上清液荧光强度分别为 283 ± 11、711 ± 19 和 622 ± 25、733 ± 25，与对照组（74 ± 5）比较，组间差异均有统计学意义（甘氨酸：F = 10.881，P = 0.024；Triton X-10：F = 12.848，P = 0.018）；而且甘氨酸与 Triton X-100两者之间有交互效应（F = 5.441，P = 0.005），其中培养基中同时含有终浓度 2% 甘氨酸及 1% Triton X-100 时培养上清液荧光强度最高（1854 ± 45），ZZ-EGFP 融合蛋白分泌表达量达到 10.4 mg/L，与对照组（0.94 mg/L）相比提高了 11 倍。 结论 甘氨酸和 Triton X-100 能提高 ZZ-EGFP 融合蛋白在液体培养基中的分泌表达量。     

黑豆汁制首乌的质量控制研究

目的　为提高制首乌的质量标准提供参考依据。方法　采用薄层色谱法 ,建立黑豆汁制首乌的鉴别方法。结果　该法操作简便 ,专属性及重现性好。结论　可作为黑豆汁制首乌的控指标之一     

Molecular dynamics and quantum chemical studies on incremental solvation of glycine

The conformational analysis of Glycine (Gly) amino acid in the presence of explicit water molecules is done using Molecular Dynamics (MD) simulation for over 10 ns time scale with step of 2 fs. Based on the hydrogen bond interactions, the solvated states of Gly and their distribution functions have been analyzed to identify the number of water molecules which fall within the first and second solvation shells. The ab initio and density functional theory (DFT) methods have been used to study the incremental solvation effect on Gly in gas phase with one to nine water molecules by constructing a hydration shell around Gly. Molecular geometries and energetical parameters of Gly?(W)_nn = 1–9 complexes were studied by MP2, B3LYP and B3PW91 methods using 6-311G (2d, 2p) basis set. The interaction energies with BSSE corrections and the strength of the hydrogen bonds have been analyzed. The chemical hardness at HF/6-311G (2d, 2p) level of theory has been calculated for all the optimized structures. The topological analysis has been carried out for the water interacting complexes using Bader’s atoms in molecule (AIM) theory. The charge transfer from the proton acceptor to the anti-bonding orbital of proton donor in Gly?(W)_nn = 1–9 complexes has been analyzed through Natural bond orbital (NBO) analysis. NMR calculations have been carried out on the basis of Cheeseman coworkers method at B3LYP/6-311G (2d, 2p) level of theory to analyse the molecular environment as well as the delocalization activities of electron cloud.     

Method development and validation for isoflavones in soy germ pharmaceutical capsules using micellar electrokinetic chromatography

The separation of six soy isoflavones (Glycitein, Daidzein, Genistein, Daidzin, Glycitin and Genistin) was approached by a 3² factorial design studying MEKC electrolyte components at the following levels: methanol (MeOH; 0–10%) and sodium dodecylsulfate (SDS; 20–70 mmol L⁻¹); sodium tetraborate buffer (STB) concentration was kept constant at 10 mmol L⁻¹. Nine experiments were performed and the apparent mobility of each isoflavone was computed as a function of the electrolyte composition. A novel response function (RF) was formulated based on the productory of the mobility differences, mobility of the first and last eluting peaks and the electrolyte conductance. The inspection of the response surface indicated an optimum electrolyte composition as 10 mmol L⁻¹ STB (pH 9.3) containing 40 mmol L⁻¹ SDS and 1% MeOH promoting baseline separation of all isoflavones in less than 7.5 min.

The proposed method was applied to the determination of total isoflavones in soy germ capsules from four different pharmaceutical laboratories. A 2 h extraction procedure with 80% (v/v) MeOH under vortexing at room temperature was employed. Peak assignment of unknown isoflavones in certain samples was assisted by hydrolysis procedures, migration behavior and UV spectra comparison. Three malonyl isoflavone derivatives were tentatively assigned. A few figures of merit for the proposed method include: repeatability (n = 6) better than 0.30% CV (migration time) and 1.7% CV (peak area); intermediate precision (n = 18) better than 6.2% CV (concentration); recoveries at two concentration levels, 20 and 50 μg mL⁻¹, varied from 99.1 to 103.6%. Furthermore, the proposed method exhibited linearity in the concentration range of 1.6–50 μg mL⁻¹ (r² > 0.9999) with LOQ varying from 0.67 to 1.2 μg mL⁻¹. The capsules purity varied from 93.3 to 97.6%.     

Colorimetric estimation of total iridoid content of Picrorhiza kurrooa

A colorimetric method has been developed for the selective analysis of the total iridoid content of the rhizomes of Picrorhiza kurrooa in terms of catalpol. The method of analysis is based on the reaction between iridoid compounds and primary amine. The iridoid glycosides present in P. kurrooa are mainly the esters of catalpol, and can be easily converted into catalpol by saponification. Catalpol thus obtained by hydrolysis is treated with glycine in acidic medium to give a purple color with a maximum absorption of 542 nm. The method was validated as per the ICH guidelines for linearity, accuracy and precision. Several other rhizome samples of the plant were also assayed using this method. The method developed is precise, sensitive, reproducible and easy to perform and can be used for the standardization of crude drug.     

淡豆豉不同制法其成品性状及异黄酮类化学成分含量的比较

目的:比较古法炮制与现今药典制法的淡豆豉其成品性状及异黄酮类化学成分的含量。方法:采用紫外-可见分光光度法对淡豆豉提取物中的总异黄酮进行含量测定;采用高效液相色谱法对淡豆豉提取物中异黄酮游离苷元(大豆素、染料木素)的含量进行测定。色谱柱为Prevail-C18(250 mm×10.0 mm,5μm),流动相为乙腈-水-乙酸(35∶64∶1,V/V/V),检测波长为260 nm,流速为1.0 ml/min,柱温为25℃,进样量为15μl。结果:古法炮制的淡豆豉气香,质地柔软,断面为棕褐色,表皮皱缩,总异黄酮含量为4.140 mg/g,大豆素含量为1.263 0 mg/g,染料木素含量为1.254 mg/g;现今药典制法的淡豆豉气味微弱,质地稍硬,断面为棕黄色,表皮皱缩,总异黄酮含量为3.530 mg/g,大豆素含量为0.349 mg/g,染料木素含量为0.335 mg/g。结论:古法炮制淡豆豉与现今药典制法淡豆豉相比,前者成品性状好、大豆异黄酮类含量高,古法炮制有明显优势。     

精氨酸-甘氨酸-天冬氨酸肽联合环氧氯丙烷改良去细胞猪瓣研究

断裂强度[(621.2±7.9)、(628.4±5.3)g/mm2,P＜0.05]和贴壁率[(0.41±0.10),(0.47±0.14),P＜0.05];光镜和电镜观察RGD+EC组种植效果优于RGD组和单纯去细胞组.结论 RGD肽联合EC改良去细胞瓣,可以提高支架的力学性能和生物学性能.     

Is glycine an inhibitory transmitter in rat lateral horn cells?

Spontaneous inhibitory postsynaptic potentials (IPSPs) and evoked IPSPs were recorded from a portion of lateral horn cells situated in thin transverse thoracolumbar spinal cord slices removed from neonatal rats. The IPSPs were reduced by hyperpolarization and inverted at membrane potentials between -65 and -75 mV. Strychnine but not bicuculline reversibly eliminated the IPSPs. The hyperpolarizations elicited by exogenously applied glycine exhibited electrophysiological and pharmacological characteristics similar to that of IPSPs. The results are consistent with the suggestion that glycine mediates an IPSP in a population of lateral horn cells.