Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells

Controlled encapsulation and pairing of single cells within a confined 3D matrix can enable the replication of the highly ordered cellular structure of human tissues. Microgels with independently controlled compartments that can encapsulate cells within separately confined hydrogel matrices would provide precise control over the route of pairing single cells. Here, a one‐step microfluidic method is presented to generate monodisperse multicompartment microgels that can be used as a 3D matrix to pair single cells in a highly biocompatible manner. A method is presented to induce microgels formation on chip, followed by direct extraction of the microgels from oil phase, thereby avoiding prolonged exposure of the microgels to the oil. It is further demonstrated that by entrapping stem cells with niche cells within separate but adjacent compartments of the microgels, it can create complex stem cell niche microenvironments in a controlled manner, which can serve as a useful tool for the study of cell–cell interactions. This microfluidic technique represents a significant step toward high‐throughput single cells encapsulation and pairing for the study of intercellular communications at single cell level, which is of significant importance for cell biology, stem cell therapy, and tissue engineering.     

一个改进的无证书签名方案

针对Yap-Heng-Goi无证书签名方案不能抵抗公钥替换攻击的缺陷,基于双线性对提出了一个改进的方案．使用短签名方案来生成用户的部分私钥,并直接和用户选取的秘密值构成签名私钥,在签名算法中使用了两个Hash函数来生成签名．由此签名的验证能够隐含地验证公钥的正确性,从而解决了Yap-Heng-Goi方案的安全问题,同时签名算法不需要对的计算,验证算法仅仅需要3个对的计算．安全性分析表明,在随机预言机模型中,计算性Diffie-Hellman假设下,这种方案能够抵抗适应性选择消息攻击下的存在性伪造．     

Low-complexity optimal spatial channel pairing for AF-based multi-pair two-way relay networks

This paper considers a multi-pair two-way relay system with multi-antenna users and proposes a low-complexity optimal spatial channel pairing （SCP） strategy at the relay station （RS） for such a system. The proposed SCP scheme, following the BD-like beamformer at RS, pairs the uplink spatial subchannels of each user pair with the corresponding downlink spatial subchannels by maximizing sum-rate （Max-SR）. Furthermore, a new mathematical inequality （arrangement inequalities of a product） is developed and applied to obtain sufficient and necessary conditions for achieving Max-SR-SCP. This also yields a simple low-complexity implementation of the proposed SCP in contrast to the NP-hard complexity of the original Max-SR-SCP problem. Simulation results illustrate that the proposed Max-SR-SCP achieves substantial sum-rate gains over other pairing schemes.     

基于配对函数的顺序相关多重数字签名

基于配对函数和陷门同态置换的签名聚合方法提出了安全的顺序相关的多重数字签名方案,具有以下特点:利用配对函数来记录签名顺序,使得签名可依序进行;签名人数不受限制;签名较短、签名和验证过程简单;无须第三方为每次签名生成密钥以及签名者的编号等。该方案可用于电子商务、电子政务中。     

Base‐Pairing Behavior of a Carbocyclic Janus‐AT Nucleoside Analogue Capable of Recognizing A and T within a DNA Duplex

Janus‐type nucleosides are heterocycles with two faces, each of which is designed to complement the H‐bonding interactions of natural nucleosides comprising a canonical Watson–Crick base pair. By intercepting all of the hydrogen bonds contained within the base pair, oligomeric Janus nucleosides are expected to achieve sequence‐specific DNA recognition through the formation of J‐loops that will be more stable than D‐loops, which simply replaces one base‐pair with another. Herein, we report the synthesis of a novel Janus‐AT nucleoside analogue, J_AT, affixed on a carbocyclic analogue of deoxyribose that was converted to the corresponding phosphoramidite. A single J_AT was successfully incorporated into a DNA strand by solid phase for targeting both A and T bases, and characterized through biophysical and computational methods. Experimental UV‐melting and circular dichroism data demonstrated that within the context of a standard duplex, J_AT associates preferentially with T over A, and much more poorly with C and G. Density functional theory calculations confirm that the J_AT structure is well suited to associate only with A and T thereby highlighting the importance of the electronic structure in terms of H‐bonding. Finally, molecular dynamics simulations validated the observation that J_AT can substitute more effectively as an A‐analogue than as a T‐analogue without substantial distortion of the B‐helix. Overall, this new Janus nucleotide is a promising tool for the targeting of A–T base pairs in DNA, and will lead to the development of oligo‐Janus‐nucleotide strands for sequence‐specific DNA recognition.     

协同OFDM系统联合中继选择、子载波配对和功率分配算法

研究了协同OFDM系统中的资源分配问题,提出了一种联合中继选择、子载波配对和功率分配的算法.首先在假定等功率分配的情况下,对中继节点进行选择并对子载波进行配对,然后在此基础上对选定的子载波对进行功率分配.分析结果显示所提算法是一种计算复杂度比较低,且系统容量接近最优容量的算法.     

一种有效可证安全的基于身份代理聚合签名方案

基于计算Diffi}Hellman问题的困难假设,构造了一个基于身份的代理聚合签名方案。该方案不仅满足代理签名的各种安全性要求,而且具有聚合签名的优点。最后,利用双线性技术对方案的正确性进行了严格的证明,并给出了方案安全性的详细证明。结果表明方案是可证安全的和可靠的,是一种安全有效的方案。     

An Improved Identity-Based Ring Signcryption Scheme

Abstract

Biometrics can be defined as science by which an individual is identified through analyses of physical data and behavioral characteristics.¹ The measurement of an individual's characteristics quantifies his or her physical, behavioral characteristics. The physically unique characteristics include, but are not limited to, fingerprints, hand or palm geometry, retina or iris scans, voice or facial characteristics, keystroke patterns, and gait. These presumably unique characteristics are used, in turn, to recognize (identify), authenticate, deny, or grant access based on the individual's characteristics.     

The consequences of pairing questions: Context effects in personality measurement.

The effect of context on responses to questions has been a prominent focus in social and political survey research. However, little investigation of context effects has been done for the measurement of psychological constructs. A measure of anger experience and expression in development uses vignettes describing interpersonal situations that provoke varying degrees of anger and require respondents to indicate their affective and/or expressive response to the situation. In this study, the consequences of pairing the two questions for each vignette are investigated. Pairing the anger-experience and likelihood-of-expression questions changes the item's context. Item response theory analysis similar to that used to detect differential item functioning was performed. For some of the items, responding to a single or paired question affected the extremity of responses. The findings are discussed in terms of their implications for personality measurement. (PsycINFO Database Record (c) 2010 APA, all rights reserved)