抗中性粒细胞胞浆抗体在系统性红斑狼疮中的研究进展

抗中性粒细胞胞浆抗体(Anti-Neutrophil Cytoplasmic Antibodies,ANCA)是一种针对单核细胞及中性粒细胞的胞浆中溶酶体酶发生反应的自身抗体。大量临床及实验证据显示ANCA不仅是ANCA相关的系统性血管炎的血清学标志,也在血管及组织损伤中起核心作用。系统性红斑狼疮(systemic lupus erythematosus,SLE)是多系统受累的自身免疫性疾病,其最特征性的损害之一是血管损伤。本文主要将近年来有关ANCA在SLE血管炎方面的研究作一详细阐述,以期为后续的临床研究提供理论依据。     

家兔输精管人工授精的精子免疫实验研究

成年雄兔１０只（Ａ组）行单侧输精管授精，１０只（Ｂ组）行双侧输精管结扎后再单侧输精管授精，１０只（Ｃ组）行双侧输精管结扎。然后按自身对照比较对三组均进行连续１２个月的抗精子抗体检测。结果表明，采用补体依赖性精子制动试验（ＳＩＴ）和微量凝集试验（ＴＡＴ）、Ａ、Ｂ组在术后３个月均测出血凝集和制动抗体；Ｃ组在术后６个月均测出血凝集和制动抗体；Ａ组２８．６％和Ｃ组２０％的家兔在术后６个月时测出精浆制动抗精     

西尼罗病毒E蛋白结构域Ⅲ特异性单克隆抗体的研制

目的研制针对西尼罗Ⅲ重组融合蛋白的特异性单克隆抗体。方法用西尼罗Ⅲ重组融合蛋白免疫BALB／c小鼠，用淋巴细胞杂交瘤技术进行细胞融合，并用间接ELISA方法进行筛选，所获得的单抗经间接ELISA、免疫印迹和间接免疫荧光试验进行鉴定。结果共获得了3株能稳定分泌针对西尼罗河病毒E蛋白结构域Ⅲ的特异性单抗的杂交瘤细胞株，分别命名为4F7、6H3和8FA，其单抗亚类鉴定分别属于IgG1、IgG1和Ig2a。这3株单克隆抗体均能与重组西尼罗河病毒E蛋白结构域Ⅲ发生特异性反应，而与日本脑炎病毒无交叉反应，并且，8FA和6H3识别同一抗原位点，4F7识别西尼罗河病毒E蛋白结构域Ⅲ的另一抗原位点。结论本实验成功研制出针对西尼罗河病毒E蛋白结构域Ⅲ的特异性单克隆抗体，为我国建立西尼罗河病毒的病原学检测方法并与日本脑炎病毒进行鉴别诊断提供了技术贮备。     

Elevated levels of IgA and IgG2 in individuals with chronic spinal cord injury

ObjectivesTo determine circulating levels of antibodies (IgA, IgM, IgG1-4) in individuals with SCI as compared to uninjured individuals.Study DesignProspective, observational study.SettingOutpatient clinic of a Department of Physical Medicine and Rehabilitation and research institute in an academic medical center.ParticipantsIndividuals with chronic (≥ 1 year from injury) SCI and uninjured individuals.Outcome MeasuresSerum antibody titers were determined by commercial multiplex ELISA.ResultsBlood samples were collected from individuals with chronic SCI (N = 29, 83% males) and uninjured individuals (N = 25, 64% males). Among participants with SCI, the distribution of American Spinal Injury Association Impairment Scale (AIS) grades was: A (n = 15), B (n = 2), C (n = 4), D (n = 8). Neurological levels of injury were: cervical (n = 17), thoracic (n = 10), and lumbar (n = 2). IgA levels were significantly elevated in participants with SCI compared to uninjured participants (median: 1.98 vs. 1.21 mg/ml, P < 0.0001), with levels most elevated in individuals with motor complete injuries compared to uninjured participants (P < 0.0003). IgG2 antibodies were also significantly elevated in participants with SCI compared to uninjured participants (median: 5.98 vs. 4.37 mg/ml, P < 0.018).ConclusionsTo our knowledge, this study provides the first evidence of elevated IgA, the antibody type most prevalent at respiratory, genitourinary and gastrointestinal tracts, common sites of infections in individuals with SCI. IgG2 levels were also elevated in individuals with SCI. These data support further investigations of IgA and other antibody types in individuals with chronic SCI, which may be increasingly important in the context of emerging novel infectious diseases such as SARS-CoV-2.     

第二次全国自身抗体检测的质控总结

目的了解我国自身抗体检测的现状,促进自身抗体检测的水平.方法以自愿报名方式确定参与单位.质控项目包括抗核抗体(ANA)、抗双链DNA抗体(抗dsDNA抗体)、抗可提取核抗原(ENA)抗体、抗线粒体抗体(AMA)、抗平滑肌抗体(ASMA)5项,共12份标本.标本发放及结果分析采用双盲操作.结果ANA正确率85.4%;抗dsDNA抗体正确率81.8%;抗ENA抗体正确率36.9%;AMA正确率66.7%、ASMA正确率50%.结论与第一次质控相比,总的来说各项抗体的检测水平有一定提高.     

Depression linked to higher antibodies production against estrogenized insulin in type 1 diabetes

Depression has been commonly associated with type 1 diabetes (T1D) and insulin covalently modified with catecholestrogens (CEs) was found in serum of these T1D patients. This study aimed to know whether depression link to higher antibodies against estrogenized insulin in T1D.ELISA (direct binding and competition) and quantitative precipitin titration were used to detect antibodies and their affinities against estrogenized insulin in the serum of 66 depressed T1D (DT1D) patients (out of 110 T1D) and 41 control subjects. Antibodies from DT1D patients showed high binding specificity to estrogenized insulin (2-hydroestradiol-insulin; 2-OHE₂-Ins) in comparison to overall T1D patients (p < 0.05) or control subjects (p < 0.001). However, T1D sera demonstrate high recognition to 2-OHE₂-Ins as compared to Ins (p < 0.05) or 2-OHE₂ (p < 0.001). The affinity of antibodies from DT1D and T1D patients was 1.32 × 10^-7 M and 1.43 × 10^-7 M, respectively. Depression linked to higher antibodies production against estrogenized insulin in T1D. Furthermore, depression in T1D generates inflammatory conditions that further increased antibodies production in T1D patients.     

贝伐珠单克隆抗体在难治性子宫颈癌中的应用进展

贝伐珠单克隆抗体（单抗）在复发性、难治性或转移性子宫颈癌中的应用逐渐增多。最早的回顾性病例报告中报道了贝伐珠单抗联合氟尿嘧啶（包括卡培他滨）或者紫杉醇对复发性或难治性子宫颈癌有一定的疗效并且药物耐受性良好。之后的Ⅱ期临床研究发现,贝伐珠单抗单剂治疗复发性宫颈鳞癌作为二线或三线治疗药物有效,耐受性良好。大型Ⅱ期和Ⅲ期临床研究进一步证实贝伐珠单抗联合化疗方案在一线、二线治疗难治性子宫颈癌可延长患者的生存期,提高患者的缓解率。此外,一部分患者对贝伐珠单抗治疗无反应或在治疗过程中会逐渐耐药,影像学检查结果和生物学标志物等可预测贝伐珠单抗的疗效。贝伐珠单抗联合化疗方案达到了常见的成本效益标准。     

抗CCP抗体、RA33抗体和IgG－Rf因子在类风湿性关节炎中的诊断价值

目的：探讨抗环瓜氨酸肽抗体（抗-CCP）、RA33抗体和类风湿因子IgG-Rf对类风湿性关节炎（RA）的临床诊断价值。方法：选择RA患者65例、非BA患者50例健康体检者50例,应用酶联免疫吸附试验（ELISA）检测抗CCP抗体、抗RA33抗体,应用免疫透射比浊法检测IgG-Rf。结果：抗CCP抗体、RA33抗体和IgG-Rf对RA诊断的敏感性分别为58．5％、33．9％、75．4％;特异性分别为98．O％、97．0％、81．0％。结论：抗CCP抗体、RA33抗体对RA诊断的特异性优于RF（P〈0．05）,可作为RA诊断的特异性血清学指标;RF与抗CCP抗体和RA33抗体同时检测用于RA的诊断是临床的最佳选择。