全文获取类型
收费全文 | 9747篇 |
免费 | 916篇 |
国内免费 | 361篇 |
学科分类
医药卫生 | 11024篇 |
出版年
2024年 | 13篇 |
2023年 | 127篇 |
2022年 | 125篇 |
2021年 | 280篇 |
2020年 | 353篇 |
2019年 | 351篇 |
2018年 | 346篇 |
2017年 | 369篇 |
2016年 | 376篇 |
2015年 | 370篇 |
2014年 | 484篇 |
2013年 | 788篇 |
2012年 | 505篇 |
2011年 | 452篇 |
2010年 | 416篇 |
2009年 | 404篇 |
2008年 | 361篇 |
2007年 | 400篇 |
2006年 | 318篇 |
2005年 | 354篇 |
2004年 | 288篇 |
2003年 | 303篇 |
2002年 | 266篇 |
2001年 | 278篇 |
2000年 | 247篇 |
1999年 | 222篇 |
1998年 | 211篇 |
1997年 | 184篇 |
1996年 | 162篇 |
1995年 | 182篇 |
1994年 | 162篇 |
1993年 | 117篇 |
1992年 | 113篇 |
1991年 | 117篇 |
1990年 | 105篇 |
1989年 | 86篇 |
1988年 | 99篇 |
1987年 | 83篇 |
1986年 | 61篇 |
1985年 | 113篇 |
1984年 | 104篇 |
1983年 | 71篇 |
1982年 | 77篇 |
1981年 | 63篇 |
1980年 | 46篇 |
1979年 | 21篇 |
1978年 | 12篇 |
1977年 | 13篇 |
1976年 | 14篇 |
1974年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
凝血酶(thromibin,Ⅱa)是一种生成于损伤处血管内皮细胞多功能蛋白酶,它是参与凝血过程各个环节反应中的关键酶。在发挥止血作用的同时,还可能诱导炎症、增生及修复等反应。最近发现的凝血酶受体(thrombin receptor,TR)分子可能为解释上述现象提供了一个理论框架。同时,TRN端被Ⅱa切下的41个氨基酸片段是否具有特殊功效,值得研究探讨。 相似文献
52.
F. Fabris I. Cordiano F. Salvan R. Ramon M. Valente G. Luzzatto A. Girolami 《European journal of haematology》1997,58(1):40-45
Abstract: We studied 47 subjects belonging to 13 unrelated families with a history of mild haemorrhagic diathesis and chronic thrombocytopenia. 36 patients presented some degree of thrombocytopenia: 7/36 (19%) had slight thrombocytopenia (100–150×109/L); 26/36 (72%) had mild thrombocytopenia (50–100×109/L) and 3/36 (8%) had severe thrombocytopenia (<50×109/L). No correlation was observed between platelet count and the degree of haemorrhagic diathesis, which was mild in the majority of patients. Transmission was autosomal dominant. Platelet anisocytosis, increased percentage of large platelets and absence of leukocyte inclusions were observed in 26/30 (87%) of the examined blood smears. The ultrastructural appearance of platelets was normal. Megakaryocytes appeared normal in number in 10/10 patients, but showed asynchronous nuclear-cytoplasm maturation and mainly nonlobulated nuclei. Platelet aggregation was studied in 26 patients and either increased or decreased curves were variably observed in response to different aggregating agents. Platelet-associated IgG (PAIgG) was increased in 18/31 (58%) patients, while serum autoantibodies against platelet glycoproteins Ib/IX or IIb/IIIa were demonstrable in only 1 case. An increased expression of platelet surface glycoproteins Ib and IIb/IIIa, as studied by murine monoclonal antibodies binding in 17 cases, was observed. Platelet survival performed by 111In-oxine-labelled autologous platelets was normal in the 3 studied patients. Congenital macrothrombocytopenia confirms to be a distinct clinical disorder for which the name of “chronic isolated hereditary macrothrombocytopenia” is proposed. 相似文献
53.
Blood platelets in severely injured burned patients 总被引:2,自引:0,他引:2
Yoshiaki Takashima 《Burns : journal of the International Society for Burn Injuries》1997,23(7-8):591-595
Unbelievable decrease of blood-platelet in the severely burned patients during the treatment of skingrafting caused two patients to unexpected death. From the records of changes of platelet number, a certain ‘platelet curve’ was made. By observing the curve, our treatments of skingrafting were carried out during the stable period and from then on we had no death cases. 相似文献
54.
Comparison of the effect of intravenous ketoprofen, ketorolac and diclofenac on platelet function in volunteers 总被引:1,自引:0,他引:1
Background : Nonsteroidal anti–inflammatory drugs (NSAIDs) inhibit prostaglandin synthesis which may result in impaired platelet function. Because NSAIDs have different abilities to inhibit cyclo–oxygenases we compared the effect of intravenous ketoprofen, ketorolac and diclofenac on platelet function in volunteers. Methods : Ten healthy male volunteers were given ketoprofen 1.4 mg kg-1, ketorolac 0.4 mg kg-1 and diclofenac 1.1 mg kg-1 in saline i.v. on three different occasions, at more than one–week intervals, in a randomized double–blind crossover study. Platelet function was evaluated before (sample 0), 2 (sample 2) and 24 h (sample 3) after the beginning of the infusion. Results : Two of the volunteers had no secondary platelet aggregation in their aggregation curves before the experiment (sample 0, studied three times) and their results were excluded from the final analysis. Diclofenac inhibited adrenaline (0.9 μg–ml-1) induced platelet aggregation less (median maximal aggregation 22.5%) than ketoprofen (18.3%) and ketorolac (15.7%) (P<0.05) in sample 2. In the ketorolac group in sample 3 an impairment of adrenaline (0.9 ng ml-1) induced platelet aggregation was still seen (26.7%) (P<0.05) but not in the other groups. Diclofenac did not affect adenosine diphosphate (ADP) induced platelet aggregation. However, ketorolac caused an impairment in ADP (3 μM and 6 μM) induced platelet aggregation and ketoprofen in ADP (6 μM) induced platelet aggregation in sample 2. Bleeding time was prolonged (P<0.05) after ketoprofen and ketorolac (sample 2) but not after diclofenac. Platelet retention on glass beads was unaffected by the tested drugs. Conclusion : Ketoprofen, ketorolac and diclofenac caused a reversible platelet dysfunction. Diclofenac had the mildest effect, while platelet dysfunction was still seen 24 h after the beginning of ketorolac. 相似文献
55.
Efficient RT-PCR on platelet mRNA after long-term storage 总被引:1,自引:0,他引:1
We have developed a procedure permitting RT-PCR from mRNA even after a long-term storage (1 year) of platelet samples in ethanol (EtOH-platelets) at −80°C. To validate our method, we have analysed the human platelet alloantigen system (HPA-1) which is coded by β3 mRNA. We have also demonstrated the efficiency of amplification of part of the coding region for (i) αIIb subunit mRNA, (ii) αv subunit mRNA, and (iii) the seven transmembrane domain thrombin receptor mRNA. 相似文献
56.
粉防己碱与牛磺酸合用对血小板聚集与血栓形成的影响 总被引:4,自引:0,他引:4
粉防己碱(Tet)和牛磺酸(Tau)均能抑制ADP、胶原和凝血酶诱导的大鼠血小板聚集及血栓形成。Tet抑制ADP诱导聚集较强,Tau则对胶原作用最明显,二药减半量合并应用时,较各药单用强 相似文献
57.
58.
Joen-Rong sheu Chao-Hsin Lin Jih-Luan chung Che-Ming Teng Tur-Fu Huang 《Thrombosis research》1992,66(6):679-691
Triflavin, an Arg-Gly-Asp (RGD)-containing peptide, purified from snake venom of Trimeresurus flavoviridis, inhibits human platelet aggregation through the blockade of fibrinogen binding to fibrinogen receptors associated with glycoprotein IIb/IIIa complex. In this report, we examined the effect of triflavin on tumor cells (human hepatoma J-5)-induced platelet aggregation (TCIPA) of heparinized platelet-rich plasma (PRP). ADP-scavenger agents, apyrase (10 U/ml) and creatine phosphate (5 mM)/creatine phosphokinase (5 U/ml) did not inhibit TCIPA while hirudin (5u/ml) completely inhibited it. J-5 cells initially induced platelet aggregation, then blood coagulation occurred. J-5 cells concentration-dependently shortened the recalcification time of normal as well as Factor VIII, IX-deficient human plasmas, while it was inactive at shortening the recalcification time of Factor VII-deficient plasma, suggesting J-5 cells induced platelet aggregation through activation of extrinsic pathway, leading to thrombin formation as evidenced by the amidolytic activity on S-2238 by expressing tissue factor-like activity. Triflavin inhibited TCIPA in a dose-dependent manner (IC50, 0.02 μM). When compared on molar ratio, triflavin was approximately 30,000 times more potent than GRGDS (IC50,0.58 mM). On the other hand, GRGES showed no significant effect on TCIPA, even its concentration was raised to 4 mM. Additionally, the monoclonal antibodies, raised against glycoprotein IIb/IIIa complex (i.e., 7E3 and 10 E5) inhibited J-5 TCIPA. In conclusion, we suggest the inhibitory effect of triflavin on J-5 TCIPA may be chiefly mediated by the binding of triflavin to the fibrinogen receptor associated with glycoprotein IIb/IIIa complex on platelet surface membrane. 相似文献
59.
BACKGROUND: It is established that the A3 domain in von Willebrand factor (VWF) contains the major collagen-binding site. However, there are conflicting reports describing the capacity of the A1 domain to interact with collagen types I and III. METHODS: In this study, we have used recombinant VWF-A1 polypeptides, as well as conformation-specific monoclonal antibodies (mAb), to analyze the A1-collagen interaction. RESULTS: The A1 domain bound to collagen with K(d) approximately 8.0 nm and this binding was blocked by the mAb 6G1, which blocks the interaction between ristocetin and VWF. In addition, collagen-bound A1 protein was able to support flow-dependent adhesion of platelets, demonstrating that the binding sites for collagen and glycoprotein (GP)Ib are different. Analysis with two conformation-specific mAb demonstrated that the structure of the A1 domain changed as a result of the binding to collagen. In contrast, the antibodies failed to detect conformational change in the G1324S mutant (type 2M von Willebrand disease). Thus, direct binding to collagen induces a change in the structural conformation within the VWF-A1 domain, and the G1324S substitution prevents this conformational change. CONCLUSION: This study has shown that the isolated A1 domain can simultaneously bind to collagen and platelet GPIb, supporting platelet adhesion under high-flow conditions. In addition, this study has used mAb to demonstrate that the binding of the isolated A1 domain or full-length VWF to collagen is accompanied by a conformational change in A1 domain. 相似文献
60.
SUMMARY. This study compared plateletpheresis on the Haemonetics PCS Plus (PCS Plus) and the Baxter Autopheresis C (Auto C) using the same 100 selected donors. The number of packs meeting UK BTS/NIBSC specification (>2.2 times 1011 platelets per pack) was achieved by 99% of PCS Plus and 82% of Auto C procedures. The positive correlation found between donor precount and final platelet yield was better for the PCS Plus. Both machines met U.K. specification for white-cell contamination but this was significantly greater for the Auto C. Plasma yields were similar.
As a result of this study we chose to use the PCS Plus for routine plateletpheresis in our unit. This has enabled us not only to comply with UK BTS/NIBSC specifications for apheresis platelets easily and cost effectively but also to meet our own higher specification (2.75 times 1011 platelets per pack) using existing staff and without extending the working day. 相似文献
As a result of this study we chose to use the PCS Plus for routine plateletpheresis in our unit. This has enabled us not only to comply with UK BTS/NIBSC specifications for apheresis platelets easily and cost effectively but also to meet our own higher specification (2.75 times 10