鱼精蛋白对刺参酸性黏多糖生物活性的影响

目的　观察鱼精蛋白对刺参酸性黏多糖 (SJAMP)抗凝血活性的对抗作用及其对SJAMP致死作用、血小板聚集、脾脏增重、抗癌活性的影响。方法　本实验测定内容包括 :凝血时间 (毛细管法 )、血小板计数 (显微镜法 )、脾脏重量、动物死亡率及动物肿瘤生长抑制率 (小鼠S180实体瘤 )。结果小鼠肌肉注射SJAMP 10 0mg kg ,凝血时间 ( 8.90± 1.85 )min ,血小板数量 ( 4 3 .40± 10 .16)× 10 1 1 个 L ,脾脏重量( 12 5 .68± 2 7.98)mg 10g ,肿瘤生长抑制率 3 2 .5 6%。小鼠肌肉注射SJAMP 10 0mg kg同时再腹腔注射鱼精蛋白 10 0mg kg ,其凝血时间 ( 3 .5 5± 1.5 2 )min(P <0 .0 1) ,血小板数量 ( 5 6.0 0± 5 .3 2 )× 10 1 1 个 L(P <0 .0 1) ,脾脏重量 ( 110 .2 0± 14 .0 9)mg 10g ,肿瘤生长抑制率 3 0 .81%。小鼠肌肉注射SJAMP 65 0mg kg ,注射后 2 4h及 48h动物死亡率分别为 9 2 0和 11 2 0 ,小鼠肌肉注射SJAMP 65 0mg kg ,再腹腔注射鱼精蛋白160mg kg× 2 ,相应的动物死亡率为 3 2 0 ( P =0 .0 3 4)和 5 2 0 ( P =0 .0 41)。结论　鱼精蛋白对SJAMP抗凝血活性及血小板聚集有明确的对抗作用 ,对SJAMP的致死性有部分保护作用 ,对SJAMP的免疫增强及抗癌活性无影响     

Surface Modification of Carbon Fiber-Polyetheretherketone Composite to Impart Bioactivity by Using Apatite Nuclei

The authors aimed to impart the apatite-forming ability to 50 wt% carbon fiber-polyetheretherketone composite (50C-PEEK), which has more suitable mechanical properties as artificial bone materials than pure PEEK. First, the 50C-PEEK was treated with sulfuric acid in a short time to form pores on the surface. Second, the surface of the 50C-PEEK was treated with oxygen plasma to improve the hydrophilicity. Finally, fine particles of calcium phosphate, which the authors refer to as “apatite nuclei”, were precipitated on the surface of the 50C-PEEK by soaking in an aqueous solution containing multiple inorganic ions such as phosphate and calcium (modified-SBF) at pH 8.20, 25 °C. The 50C-PEEK without the modified-SBF treatment did not show the formation of apatitic phase even after immersion in simulated body fluid (SBF) for 7 days. The 50C-PEEK treated with the modified-SBF showed the formation of apatitic phase on the entire surface within 1 day in the SBF. The apatite nuclei-precipitated 50C-PEEK will be expected as a new artificial bone material with high bioactivity that is obtained without complicated fabrication processes.     

Thyroid hemiagenesis and elevated thyrotropin levels in a child with Williams syndrome

A girl with Williams syndrome (WS) presented with elevated thyrotropin (TSH) levels (7.0 μU/ml), normal free thyroid hormone concentrations, and absent antithyroid autoantibodies. Thyroid ultrasonography and scintigraphy showed hemiagenesis of the left lobe and no evidence of ectopic tissue. TSH response to thyrotropin-releasing hormone (TRH) injection (200 μg/mq, i.v.) was exaggerated and prolonged, suggesting subclinical hypothyroidism. The biological activity of circulating TSH was slightly below the normal range [TSH bioactivity (B) to immunoreactivity (I) ratio (TSH B/I) = 0.4, normal: 0.6–2.2]. These abnormalities are similar to those seen in patients with hypothalamic hypothyroidism. Thyroid function is not a recognized manifestation of WS and is not routinely investigated. However, abnormalities of the hypothalamic-pituitary-thyroid (HPT) axis and thyroid dysgenesis have been found in other WS cases. Genes mapping at 7q11.23, contiguous to the chromosomal region deleted in most WS patients, may be involved in the development of the thyroid gland, contributing to the complex phenotype of WS. Am. J. Med. Genet. 85:491–494, 1999. © 1999 Wiley-Liss, Inc.     

猫爪草多糖RTG-Ⅲ的分离纯化及其生物活性

 目的 研究中药猫爪草水提取液中分离纯化得到一种多糖蛋白复合物——RTG-Ⅲ的生化特性、免疫和抑瘤作用。方法 采用DEAE-Cellulose-52和Superdex 200柱色谱得到均一的多糖组分,电泳和凝胶色谱等方法检测纯度,薄层色谱和气相色谱分析单糖组成,红外光谱确定糖苷键类型,噻唑蓝试验测定免疫活性。结果 RTG-Ⅲ为均一组分,单糖组成为鼠李糖、阿拉伯糖、葡萄糖、半乳糖、甘露糖,其摩尔比为1.00:1.17:51.71:2.16:0.46,总糖含量为80.1％,蛋白含量为12.6％,由谷氨酰胺、谷氨酸等16种氨基酸组成,糖肽键为非O-型。结论 RTG-Ⅲ是以β-D-葡萄吡喃糖为主的一种新的多糖-蛋白复合物,相对分子质量为2.3×10⁴,能够增强免疫细胞对肿瘤细胞HL-60的抑制作用。     

Resorbable Mg2+-Containing Phosphates for Bone Tissue Repair

Materials based on Mg²⁺-containing phosphates are gaining great relevance in the field of bone tissue repair via regenerative medicine methods. Magnesium ions, together with condensed phosphate ions, play substantial roles in the process of bone remodeling, affecting the early stage of bone regeneration through active participation in the process of osteosynthesis. In this paper we provide a comprehensive overview of the usage of biomaterials based on magnesium phosphate and magnesium calcium phosphate in bone reconstruction. We consider the role of magnesium ions in angiogenesis, which is an important process associated with osteogenesis. Finally, we summarize the biological properties of calcium magnesium phosphates for regeneration of bone.     

Design,Synthesis, and Antitumor Activity of 4-Halocolchicines and Their Pro-drugs Activated by Cathepsin B

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