骨髓基质细胞体外培养的生物学特性和成骨能力初步鉴定

目的体外分离培养狗的骨髓基质细胞（BMSCs），诱导其向成骨细胞分化，初步鉴定其成骨潜能和生物学特性。方法抽取毕格犬髂骨骨髓体外分离培养获得BMSCs，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10mmol／L地塞米松、50μg／ml抗坏血酸和10mmol／L β-甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化。倒置相差显微镜进行细胞形态学和细胞生长增殖观察，VonKossa法染色检测体外矿化结节形成，细胞碱性磷酸酶染色检测BMSCs的成骨活性。结果体外分离培养的BMSCs经条件培养基诱导后表现出明显的成骨活性，体外矿化（骨样）结节的Von Kossa染色阳性；传代BMSCs碱性磷酸酶染色阳性。结论体外分离培养的BMSCs中含有骨源性前体细胞．传代细胞具有较强的成骨潜能。     

近五年木兰科植物生物活性研究及应用进展

综述了近5年来国内外有关木兰科植物生物活性的研究及应用进展，尤其是在抗肿瘤、抗氧化、抗炎、对中枢神经系统作用及其他生物活性、 美容护肤化妆品等方面的研究及应用情况。     

Methods to study the phytochemistry and bioactivity of essential oils

Many essential oils are extracted, analysed and their main components are identified, characterised and then published without any biological testing whatsoever. Their useful biological activities can remain unknown for years. Yet, the search for these activities often increases our knowledge of the potential use of oils in therapeutics. Therefore, there is a real need for a simple, reliable and reproducible methods to study the bioactivity of essential oils and their constituents which can detect a broad spectrum of action or specific pharmacological activities in aromatic plants. These methods can then be employed by natural product chemists, pharmacologists and biologists to conduct their scientific research and to valorize natural products. Standardisation of some of these methods is therefore desirable to permit more comprehensive evaluation of plant oils, and greater comparability of the results obtained by different investigators.     

Systemic bioactivity of intranasal triamcinolone and mometasone in perennial allergic rhinitis

AIMS: To evaluate the systemic bioactivity of triamcinolone acetonide (TA) 220 micro g or mometasone furoate (MF) 200 micro g over 3 weeks in perennial allergic rhinitis. METHODS: Twenty-seven patients received TA 220 micro g or MF 200 micro g once daily for 3 weeks with a 2 week placebo washout period prior to each randomized treatment. Measurements were made at baseline after each washout and after each randomized treatment, comprising overnight 10-h urinary cortisol corrected for creatinine (OUCC), 08.00 h plasma cortisol and 08.00 h serum osteocalcin. RESULTS: There were no significant differences between baseline values prior to TA or MF, and for any outcome measures comparing randomized treatments to respective baseline values or comparing TA with MF. For OUCC compared with baseline, the geometric mean fold suppression (95% CI) was 1.02 (0.78, 1.33) for TA (2% decrease), 1.07 (0.80, 1.42) for MF (7% decrease), and 1.05 (0.79, 1.39) for TA vs MF (5% decrease). CONCLUSIONS: Standard doses of TA or MF over 3 weeks showed no differences in systemic bioactivity markers compared with respective baseline values after placebo washout, and there were no differences between TA vs MF.     

6,7-二甲氧基-4-芳胺基喹唑啉衍生物的合成与活性筛选

 目的合成6,7-二甲氧基-4-芳胺基喹唑啉衍生物及其抗肿瘤活性筛选。方法以2-氨基-4,5-二甲氧基苯甲酸和醋酸甲脒为原料,合成了10个4位芳胺取代的6,7-二甲氧基喹唑啉衍生物,利用酶联免疫吸附分析法(ELISA)PTK-101试剂盒并对化合物进行活性筛选。结果合成的化合物经IR,¹H-NMR,MS结构表征。初步的生物活性测试表明,这类化合物对表皮生长因子受体(EGFR)酪氨酸激酶有明显的抑制活性。其苯环4′位的取代基变化对活性具有显著影响。结论6,7-二甲氧基-4-芳胺基喹唑啉衍生物可能会在治疗肿瘤特别是非小细胞肺癌方面发挥更大。     

基因重组神经营养蛋白-3的生物学活性

目的：测定ＮＴ－３ｃＤＮＡ／ＣＨＯ细胞表达的基因重组ＮＴ－３的生物学活性。方法：体外培养８日龄鸡胚背根神经节（ＤＲＧ）,加入条件培养基,测量背根节神经突起生长的长度。结果：ＮＴ－３ｃＤＮＡ／ＣＨＯ细胞培养４８ｈ后的无血清条件培养基１：２４稀释后即能促进ＤＲＧ神经突破的生长;随着其中ＮＴ－３浓度增加,神经突破长度也逐渐延长,有明显的量效关系;ＤＲＧ培养４８ｈ长出的神经突破与２４ｈ相比,明显长而致密;     

全反式维甲酸四乙酰葡萄糖酯的合成及急性皮肤刺激性研究

全反式维甲酸在４－二甲氨基吡啶催化下,与２,３,４,６－四－Ｏ－乙酰－α－Ｄ－吡喃葡萄糖溴化物反应,生成全反式维甲酸四乙酰葡萄糖酯（１）,收率５８％。急性皮肤刺激试验表明：１对皮肤刺激性小于全反式维甲酸（２）,而且保持与全反式维甲酸一样的生物活性。     

唇形科香茶菜属二萜类化合物的生物活性研究进展

香茶菜属植物资源丰富,所含化学成分以二萜类化合物为主。二萜类化合物具有广泛的生物活性,以抗肿瘤、抗炎和抑菌活性最为显著。通过生物活性的广泛筛选和作用机制研究,该类化合物结构的活性基团和作甩机制被逐步揭示,而这些研究结果为香茶菜属植物进一步研究开发提供了科学依据。本文对该类化合物的研究进展作一综述。     

高活力人降钙素类似物vhCT的免疫原性研究

本研究用固相多肽合成法合成人降钙素类似物vhCT ,产物纯度达到等电聚焦均一 ,质谱测定分子量精确。生物活性为天然人降钙素的 2 0～ 2 5倍。用放射免疫法测定vhCT能与识别天然人降钙素的抗体起反应 ,产生IgG抗体的免疫原性随偶联的不同载体而有差异。但在BALB/C5 7小鼠体内不引起IgE增高。提示vhCT不产生IgE介导的过敏反应 ,具有药物应用前景。     

基于神经网络的方法预测生物活性介质网络调控矽肺纤维化的研究

目的:探讨矽肺纤维化同生物活性介质之间的关系。方法:利用Delphi语言编制了BP人工神经网络模型计算机程序,建立并分析了矽肺胶原纤维预测的数学模型。结果:选定网络隐含层节点为9,初始权值阈值约为(-0.2,0.2),最大相对误差为4%,最小相对误差为0.2%。结论:应用神经网络具有较好的预测效果,可为临床医学研究提供一个很好的研究思路。