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41.
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采用改良的溶胶-凝胶法制备系列的As2O3纳米粒,用透射电镜、扫描电镜、能谱仪、图像分析系统等进行表征及特性检测.应用MTT法研究As2O3纳米粒体外对细胞的增殖抑制作用;用流式细胞仪测定As2O3纳米粒及亚砷酸诱导细胞的凋亡率;用免疫组织化学半定量法检测As2O3纳米粒及亚砷酸处理细胞后Bcl-2、Bax、CD44v6和P53基因的表达改变.研究结果表明,制备的As2O3纳米粒在电镜下呈圆形或椭圆形,分散性较好,平均直径约为80nm、110nm、130nm、150nm和450nm;体外细胞实验证实As2O3纳米粒抗肺癌A-549细胞的效应强于亚砷酸溶液;免疫组织化学半定量法显示As2O3纳米粒有较强的诱导肺癌细胞凋亡的作用,可能与其改变Bcl-2和Bax基因表达(Bcl-2/Bax比值降低)及促进P53基因的表达、抑制CD44v6基因表达有关. 相似文献
43.
ANSYS软件是通用大型有限元分析软件,网格划分是有限元分析的重要环节,也是影响分析精度和求解规模的一个因素,ANSYS的前处理程序常应用MESH200单元来划分网格,结合实例来说明MESH200单元的用法和特点,为几何实体划分网格提供一个参考。 相似文献
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为了能在人口集中的公共场所快速、准确测量大批量人群体温,系统采用RE200B红外传感器作为温度采集器件,以STC89C52为核心,DS18B20传感器采集环境温度作为补偿温度,设计了一种电子红外测温仪.系统主要包括硬件和软件两大部分,红外信号经过光学系统聚焦在RE200B热释电探测器上并转变为相应的电信号,此信号经过放大、A/D转换,再送到单片机中进行数据的处理、补偿,最后送到液晶显示结果.软件采用C语言来编写.经实验测试:设计红外体温仪平均相对误差小于0.3%,符合系统设计的预期要求. 相似文献
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根据嵌入式系统电源的实际需求,以FSD200小功率单片开关电源集成电路为核心,结合可调式精密稳压器TL431和线性光耦PC817等外围器件,设计并实现了适合嵌入式系统使用的多路小功率开关电源。 相似文献
48.
利用CFX-TASCflow软件对200MW汽轮机低压缸各级进行优化设计。计算结果对比表明,优化后汽流流动均有改善,各级效率均有提高。 相似文献
49.
Michela Pecoraro Stefania Marzocco Silvia Franceschelli Ada Popolo 《International journal of molecular sciences》2022,23(12)
Human epidermal growth factor receptor-2 (HER2) is overexpressed in up to 30% of breast cancer cases, causing a more aggressive tumour growth and poor prognosis. Trastuzumab, the humanized antibody targeted to HER2, increased the life expectancy of patients, but severe cardiotoxicity emerged as a long-term adverse effect. Clinical evidence highlights that Trastuzumab-induced cardiotoxicity drastically increases in association with Doxorubicin; however, the exact mechanisms involved remain incompletely understood. In order to analyse the molecular mechanisms involved and the possible adaptative responses to Trastuzumab and Doxorubicin treatment, in this study, H9c2 cardiomyoblasts were used. Results showed that Trastuzumab and Doxorubicin sequential administration in cardiomyoblast increased cytosolic and mitochondrial ROS production, intracellular calcium dysregulation, mitochondrial membrane depolarization, and the consequent apoptosis, induced by both Trastuzumab and Doxorubicin alone. Furthermore, in these conditions, we observed increased levels of Connexin43 phosphorylated on Ser368 (pCx43). Since phosphorylation on Ser368 decreases gap junction intracellular communication, thus reducing the spread of death signals to adjacent cells, we hypothesized that the increase in pCx43 could be an adaptative response implemented by cells to defend neighbouring cells by Trastuzumab and Doxorubicin sequential administration. However, the other side of the coin is the resulting conduction abnormalities. 相似文献
50.
Afroditi Kastrenopoulou Kyriakos E. Kypreos Nicholaos I. Papachristou Stavros Georgopoulos Ioulia Mastora Ioanna Papadimitriou-Olivgeri Argyro Spentzopoulou Dragana Nikitovic Vassilios Kontogeorgakos Harry C. Blair Dionysios J. Papachristou 《International journal of molecular sciences》2022,23(9)
In the present study, we studied the effect of apolipoprotein A-1 (APOA1) on the spatial and molecular characteristics of bone marrow adipocytes, using well-characterized ApoA1 knockout mice. APOA1 is a central regulator of high-density lipoprotein cholesterol (HDL-C) metabolism, and thus HDL; our recent work showed that deficiency of APOA1 increases bone marrow adiposity in mice. We found that ApoA1 deficient mice have greatly elevated adipocytes within their bone marrow compared to wild type counterparts. Morphologically, the increased adipocytes were similar to white adipocytes, and displayed proximal tibial-end localization. Marrow adipocytes from wild type mice were significantly fewer and did not display a bone-end distribution pattern. The mRNA levels of the brown/beige adipocyte-specific markers Ucp1, Dio2, Pat2, and Pgc1a; and the expression of leptin were greatly reduced in the ApoA1 knock-out in comparison to the wild-type mice. In the knock-out mice, adiponectin was remarkably elevated. In keeping with the close ties of hematopoietic stem cells and marrow adipocytes, using flow cytometry we found that the elevated adiposity in the ApoA1 knockout mice is associated with a significant reduction in the compartments of hematopoietic stem cells and common myeloid, but not of the common lymphoid, progenitors. Moreover, the ‘beiging’-related marker osteopontin and the angiogenic factor VEGF were also reduced in the ApoA1 knock-out mice, further supporting the notion that APOA1—and most probably HDL-C—regulate bone marrow microenvironment, favoring beige/brown adipocyte characteristics. 相似文献