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101.
Three-dimensional (3-D) cell morphology is important for the understanding of cell function and can by quantified in terms of volume and surface area. Differential interference contrast (DIC, or Nomarski) imaging can enable cell edges to be clearly visualized in unstained tissue due to the slight difference in refractive index between aqueous media and cytoplasm. DIC is affected in only one direction - the direction of the optical shear. A 1-D edge detector was used in that direction with a scale length equal to that of an in-focus edge to highlight cell boundaries. By comparison with the signal from the edge detector on an out-of-focus slice, the in-focus slices could be segmented and, after noise suppression, cell outlines obtained. A voxel paradigm was used to calculate cell volume and differential geometry was used for surface area estimation. We applied this approach to obtain 3-D dimensional information by optical sectioning of motile Amoeba proteus.  相似文献   
102.
Higher resolution can be achieved in lithography by decreasing the wavelength of the exposure source. However, resist material and their processing are also important when we move to a shorter wavelength lithography technology. This paper reviews the recent development and challenges of deep-UV photoresists and their processing technology.  相似文献   
103.
A new all-spectroscopic method for depth-resolved thermal diffusivity measurement of metallic specimens has been demonstrated. The method entails measurement of the mass entrained into a laser-produced plasma (LPP) plume in such a manner that the plume is representative of the specimen in elemental composition. Both the abundance of matter and its elemental composition are measured by time-resolved spectroscopy for each LPP plume. In order to delineate the morphology versus composition basis of the depth dependence, a new study on a Nichrome ribbon specimen heated by ohmic heating in a vacuum is presented. A set of depth-resolved thermal diffusivity measurements is carried out, while noting the attendant changes in the spectral emissivity and elemental composition at succeeding ablation layers. Additional measurements are carried out after the specimen has been treated under varying heating conditions. Preferential diffusion of chromium at high temperatures has been found to contribute to the dynamics of surface thermophysical properties at high temperatures. Representative LPP ablation is well suited for removal of surface impurities prior to thermophysical property measurements by the pulse heating technique.  相似文献   
104.
大曲率表面物体形貌的光学检测方法研究   总被引:13,自引:7,他引:6  
本文提出了两种用于曲面形貌检测的傅里叶变换法中的频域低通滤波器,并与常用的窗口法低通滤波器进行了比较,给出了实际测量的结果。这两种频域低通滤波器(高幂次指数滤波器和矩形余弦滤波器)有可方便调整且平直的通带,平直无旁瓣的阻带,较陡的边带将通带与阻带间圆滑过渡连接,从而可尽可能多地保留有用频率成分,很好地滤掉直流和不用的高频分量,且较好地抑制振铃现象,克服一般窗口法设计的低通滤波器中旁瓣的影响。具有使用简单、参数调整方便、应用范围广等特点,尤其是对具有大梯度表面和棱角细节的物体检测更有独到之处,具有广泛的应用前景。  相似文献   
105.
总结了历年长贮试验情况,分析了防腐层变化的原因,指出了引信零件在选择表面处理方式时应注意的问题。  相似文献   
106.
The alkali-metal Na adsorption on Si(100)2×1 surface and its promoted oxidation and Si oxidegrowth have been investigated by means of thermal desorption,work function,Auger electronspectroscopy and photoemission electron spectroscopy.The experimental data showed that therewas a new state,interface electron state,near the Fermi level after the deposition of Na atoms.It wasfound that the presence of Na always caused an increase of the oxygen initial uptake whereas thepromotion of Si oxide growth was observed only at the coverage of Na greater than 0.5 ML.A newmechanism of Na-promoted Si oxide growth is suggested in this paper.  相似文献   
107.
离子注入对Cr12MoV钢表面组织结构和力学性能的影响   总被引:1,自引:0,他引:1  
研究了Cr12MoV钢离子注入层的显微组织特征及其对表面性能的影响。结果表明,离子注入使Cr12MoV钢的表面硬度和耐磨性能得到显著改善,且存在着一个最佳的注入剂量,约为3×10^1^7N^+/cm^2左右。离了注入在Cr12MoV钢表面层中形成了大量细小弥散的第二相粒子,并使α-Fe晶格发生严重畸变,从而引起材料表面强化。  相似文献   
108.
基底元素表面富集与扩散阻档层   总被引:1,自引:0,他引:1  
根据An-Ag系统以及An-Cu系统的扩散系数计算,提出了扩散阻挡层模型。利用这个模型对Au-Ag系统进行有关实验,得利了较好的验证。  相似文献   
109.
Recently, superconducting Nd1Ba2Cu3Oy (Ndl23) thin films with high superconducting transition temperature (T c) have been successfully fabricated at our institute employing the standard laser ablation method. In this paper, we report the results of surface characterization of the Nd123 thin films using an ultrahigh vacuum scanning tunneling microscope/spectroscopy (UHV-STM/STS) and an atomic force microscope (AFM) system operated in air. Clear spiral pattern is observed on the surfaces of Nd123 thin films by STM and AFM, suggesting that films are formed by two-dimensional island growth mode. Contour plots of the spirals show that the step heights of the spirals are not always the integer or half-integer number of thec-axis parameter of the structure. This implies that the surface natural termination layer of the films may not be unique. This result is supported byI-V STS measurements. The surface morphology of the Nd123 thin films is compared with that of thec-axis-oriented Y1Ba2Cu3Sy thin films. Surface atomic images of the as-prepared Nd123 thin films are obtained employing both STM and AFM. STS measurements show that most of the surfaces are semiconductive. The results of STS measurements together with the fact that we are able to see the surface atomic images using scanning probe microscopes suggest that exposure to air does not cause serious degradation to the as-prepared surfaces of Nd123 thin films.  相似文献   
110.
Inverse surface design problems from light transport behavior specification usually represent extremely complex and costly processes, but their importance is well known. In particular, they are very interesting for lighting and luminaire design, in which it is usually difficult to test design decisions on a physical model in order to avoid costly mistakes. In this survey, we present the main ideas behind these kinds of problems, characterize them, and summarize existing work in the area, revealing problems that remain open and possible areas of further research.  相似文献   
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