miR-216a-5p在HMGB1介导的表皮葡萄球菌感染致腹膜透析相关腹膜炎中的作用

 目的 探讨miR-216a-5p在高迁移率蛋白B1（HMGB1）介导的表皮葡萄球菌感染致小鼠腹膜透析相关腹膜炎（PDAP）中的作用及机制。方法 选取健康雄性C57BL/6J小鼠,随机分为对照组、感染组、感染+HMGB1抑制剂组,收集腹腔积液及腹膜组织分别进行白细胞计数、HE和免疫组织染色;Real-time PCR和Western Blot检测白细胞介素-1α（IL-1α）、白细胞介素-6（IL-6）、肿瘤坏死因素-α（TNF-α）、HMGB1、核转录因子-κB （NF-κB）和核转录因子抑制蛋白（I-κB）的mRNA和蛋白表达水平;生信预测发现,miR-216a-5p可能与HMGB1结合,参与其诱导的PDAP的发生,并构建感染+miR-216a-5p mimics组小鼠,通过双荧光素酶报告基因检测验证miR-216a-5p和HMGB1的关系,采用Real-time PCR、Western Blot、免疫组织染色检测HMGB1的表达变化。结果 与对照组相比,感染组小鼠腹腔积液白细胞计数增多,炎性浸润明显,IL-1α、IL-6、TNF-α,以及HMGB1 mRNA和蛋白表达均升高（均P<0.05）;HMGB1抑制剂（甘草素）干预后,小鼠腹腔积液白细胞计数下降,炎性浸润改善,IL-1α、IL-6、TNF-α,以及HMGB1 mRNA和蛋白表达均下降（均P<0.05）。感染组小鼠NF-κB表达高于对照组,I-κB表达低于对照组;HMGB1抑制剂干预后NF-κB表达降低,I-κB表达升高（均P<0.05）。Real-time PCR结果证实,与对照组相比,miR-216a-5p的含量在感染组中显著减少;双荧光素酶报告基因检测提示,miR-216a-5p可直接作用于HMGB1的3’UTR区;与感染组相比,感染+miR-216a-5p mimics组小鼠中HMGB1 mRNA和蛋白表达均下降（均P<0.05）。结论 HMGB1在表皮葡萄球菌感染致小鼠PDAP中发挥重要作用,抑制HMGB1可改善小鼠炎症反应,miR-216a-5p可通过靶向HMGB1参与表皮葡萄球菌感染致PDAP的发生。     

胃镜治疗与内科治疗对消化性溃疡出血的应用效果比较分析

目的:探究胃镜治疗与内科治疗对消化性溃疡出血的应用效果对比。方法:在2018年9月~2019年9月这个时间段中,选取本院收治的45例消化性溃疡出血患者作为本次研究的观察组,给予胃镜治疗;同期选取本院收治的45例消化性溃疡出血患者作为本次研究的对照组,给予内科治疗;对两组疗效进行对比。结果:观察组治疗总有效率(95.6%)显著高于对照组(77.8%),有统计学意义,P<0.05。观察组住院时间、止血时间与再次出血率均优于对照组,P<0.05。结论:在消化性溃疡出血患者中采用胃镜治疗的效果优于内科治疗效果,且止血效果较好,促使患者尽早康复,在临床上的应用价值较高。     

呼吸性煤尘测试方法在包钢焦化厂的应用

目的：呼吸性煤尘测试方法是否与总尘的测试方法所得的结果一致。方法：根据GB16248－1996《作业场所空气中呼吸性煤尘卫生标准》,测定呼吸性煤尘浓度。根据GB5748－85《作业场所空气中粉尘测定方法》测定总尘浓度,结果：呼吸性煤尘浓度的合格率与总煤尘浓度的合格率之间无显著的差异。总煤尘浓度与呼吸性煤尘浓度之比波动在1．52－3．33之间。结论：呼吸性煤尘测试方法与总尘的测试方法所得的结果一致。     

胸腰椎骨折内固定技术和应用现状

应用内固定技术治疗胸腰椎骨折,目前已被国内外广泛采用,并经过不断的改进,取得了良好的疗效,笔者就目前开展的内固定器械及技术,内固定方法的生物力学,临床应用效果及内固定术的失误,并发症作一简要综述。     

药用辅料在制剂中的应用概述

药用辅料是药物制剂的基础材料的重要的组成部分,在制剂型和生产中起着关键作用,它不仅赋予药物一定剂型,并且与提高药物的疗效,降低毒副作用有很大的关系,因此,研究开发,合理应用辅仅可提高药物制剂质量和生产技术水平,而且可取得较大的社会及经济效益。     

纳络酮治疗急性心肌梗塞并心源性休克18例临床观察

目的：了解纳络酮在心急性心肌梗塞（AMI）并心源性休克中的作用及对其预后的影响。方法：收集我院近4年来AMI并发心源性休克的患者35例,随机分普通组17例和纳络酮组18例进行救治,结果：常规组有效率为29．4％,纳络酮组有效率为61．1％,经x^2检验,P＜0．05,有显著性差异,结论：AMI并心源性休克在无条件实施急诊血运重建术的医院,在常规治疗的基础上加用纳络酮治疗,可显著提高有效率,降低死亡率。     

大鼠肝肠联合移植对移植肠内ATP酶、AKP和AChE活性的影响

采用肝/小肠联合移植模型,观察对移植肠ATPase、AKP和AChE活性的影响,结果发现肝小肠联合移植与单纯小肠移植相比①前者能延长受体动物的存活期,并对移植小肠产生的免疫排斥反应有明显的减轻和延迟作用;②肝小肠联合移植的移植物内ATPase、AKP和AChE活性反应较好。结果表明上述3种酶活性的改变能相应地反映移植器官产生免疫排斥反应的程度和受体动物的生存状态,并可以作为器官移植后临床监测中的一种辅助措施。     

索拉非尼联合免疫检查点抑制剂治疗TACE抵抗的中晚期肝癌的疗效及安全性

目的 探讨索拉非尼联合免疫检查点抑制剂（immune checkpoint inhibitors,ICIs）治疗经肝动脉化疗栓塞术（transcatheter arterial chemoembolization,TACE）抵抗的中晚期肝癌的疗效及安全性。方法 回顾性分析2017年1月至2021年3月期间在温州医科大学附属第五医院确诊为TACE抵抗的中晚期肝癌患者的资料,共54例,年龄28～82（56.1±11.8）岁,其中BCLC B期24例,C期30例;Child-Pugh A级34例,B级20例。根据治疗方法不同,分为对照组和联合组,对照组接受单一索拉非尼治疗（n=33）,联合组接受索拉非尼联合ICIs治疗（n=21）;比较两组患者的一般情况、肿瘤反应性、肿瘤无进展生存时间（progression-free survival,PFS）及不良反应的发生情况。结果 联合组（2例CR、11例PR、4例SD和4例PD）的肿瘤反应性明显优于对照组（2例CR、8例PR、10例SD、13例PD）（P=0.041）。联合组的中位PFS明显长于对照组（13.5个月 vs 6.8个月,P=0.010）;多因素分析显示Child-Pugh B级（HR 2.202,95%CI 1.059～4.581,P=0.035）、BCLC C期（HR 4.077,95%CI 1.902～8.742,P<0.001）、AFP≥400 ng/mL（HR 5.728,95%CI 2.227～14.729,P＜0.001）是TACE抵抗肝癌PFS的独立危险因素,索拉非尼联合ICIs（HR 0.180,95%CI 0.082～0.392,P<0.001）是肝癌患者的保护因素。联合组并发皮炎、腹泻的发生率明显多于对照组（P<0.05）。结论 索拉非尼联合ICIs治疗提高TACE抵抗中晚期肝癌的肿瘤反应性,延长患者PFS,是一种有效、安全的治疗方案。     

Therapeutic effects of the combined androgen blockade therapy versus luteinizing hormone-releasing hormone analog monotherapy in patients with hormone naïve metastatic prostate cancer: a multi-institutional comparative analysis

BackgroundThe clinical benefit of the combined androgen blockade (CAB) therapy over luteinizing hormone-releasing hormone analog (LH-RHa) monotherapy for hormone naïve metastatic prostate cancer (mHNPC) is unclear. Therefore, we retrospectively compare the effectiveness of CAB with the LH-RHa monotherapy on the prognosis of Japanese patients with mHNPC.MethodsWe retrospectively evaluated the prognosis of 517 patients diagnosed with mHNPC between August 2001 and May 2017. The patients’ data were obtained from the Michinoku Urological Cancer Research Group database and Hirosaki University-related hospitals. Patients were divided into the CAB and LH-RHa monotherapy groups based on primary androgen deprivation therapy (ADT). Overall survival (OS), cancer-specific survival (CSS), and castrate-resistant prostate cancer-free survival (CRPC-FS) were compared between the two groups using the Kaplan-Meier curve analysis. Inverse probability of treatment weighting (IPTW)-adjusted Cox hazard proportional analyses was performed to investigate the effect of primary ADT on oncological outcomes.ResultsThe median age was 73 years old. The numbers of patients in the CAB and LH-RHa monotherapy groups were 447 and 70, respectively. The Kaplan-Meier curve analysis showed no significant differences in either 5-year OS (56.7% vs. 52.5%, P=0.277), CSS (61.1% vs. 56.4%, P=0.400), and CRPC-FS (33.1% vs. 31.1%, P=0.529) between the groups. IPTW-adjusted multivariate Cox hazard proportional analyses showed no significant differences in OS, CSS, and CRPC-FS between the two groups.ConclusionsNo significant differences in oncological outcomes were observed between the CAB and LH-RHa monotherapy groups in patients with mHNPC.     

Analysis of types of chromosomal aberrations following the combined action of chemical mutagens

Types of chromosomal aberrations in cultures of human lymphocytes exposed to the combined action of various concentrations of thiophosphamide and dipin, with different proportions of each, were studied. The mutagens acted on the G₀ stage. The range of concentrations used was from 3.17·10^–5 to 22.19·10^–5M. Equimolar concentrations of thiophosphamide inhibited more chromatid exchanges and fewer sister-strand (isolocus) unions than dipin, and it also induced a greater proportion of single breaks and a greater proportion of breaks in chromatid exchanges relative to the total number of chromosome breaks. Both the absolute and the relative frequencies of chromosomal aberrations depended on the concentration of the mutagens. A change in the ratio between thiophosphamide and dipin, if the total number of molecules of the two mutagens at the different concentration levels remained constant, gave rise to an effect whose level was between the effects of action of equimolar concentrations of the pure mutagens. This effect depended on the proportion of each mutagen in the combined treatment. It is concluded that the action of thiophosphamide and dipin is additive.Laboratory of Mutagenesis, Institute of Medical Genetics, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. V. Smol'yannikov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 85, No. 1, pp. 79–81, January, 1978.