绿茶及其有效成分对肾性高血压大鼠左心室肥厚的抑制作用

目的研究绿茶及其有效成分对肾性高血压大鼠左心室肥厚的抑制作用。方法雄性Wistar大鼠160只,体重180～220g,随机分为5组,建立二肾一夹法大鼠肾性高血压左心室肥厚模型。假手术组(Sham),正常饮水;阳性对照组(二肾一夹法,2K1C),正常饮水;绿茶组(二肾一夹法加绿茶水,2K1C+GT),饮用2%绿茶水;茶多酚组(二肾一夹法加茶多酚水,2K1C+TP),饮用0.1%茶多酚水;表没食子儿茶素没食子酸酯(EGCG)组(二肾一夹法加EGCG水,2K1C+EGCG),饮用0.05% EGCG水,术后即给予不同浓度的绿茶及其提有效成分,持续8周。分别于实验第0、4、8周测量血压。8周后处死测量左心室与体重比值(LVW/BW)和左心室壁厚度(LVWT),检测胚胎基因-心房利钠肽(embryonic gene atria natriuretic peptide,ANP),并对部分心肌组织HE染色进行病理组织学观察。结果与假手术组比较,阳性对照组和各饮茶组血压、LVW/BW和LVWT均显著升高(P<0.01)。而与阳性对照组相比,不同浓度的绿茶及其有效成分(2%绿茶、0.1%茶多酚、0.05% EGCG)LVW/B...     

Non-targeted analysis of tea by hydrophilic interaction liquid chromatography and high resolution mass spectrometry

Tea is the second most consumed beverage in the world and its consumption has been associated with numerous potential health benefits. Factors such as fermentation methods, geographical origin and season can affect the primary and secondary metabolite composition of tea. In this study, a hydrophilic interaction liquid chromatography (HILIC) method coupled to high resolution mass spectrometry in both positive and negative ionisation modes was developed and optimised. The method when combined with principal component analysis to analyse three different types of tea, successfully distinguished samples into different categories, and provided evidence of the metabolites which differed between them. The accurate mass and high resolution attributes of the mass spectrometric data were utilised and relative quantification data were extracted post-data acquisition on 18 amino acids, showing significant differences in amino acid concentrations between tea types and countries. This study highlights the potential of HILIC chromatography combined with non-targeted mass spectrometric methods to provide a comprehensive understanding of polar metabolites in plant extracts.     

脂溶性儿茶素类化合物的制备研究进展

儿茶素类化合物是一类具有多种生理活性的多酚类抗氧化剂,本文综述了国内外利用分子修饰法制备脂溶性儿茶素类化合物的进展。化学法主要利用酰氯在化学催化剂作用下反应制备脂溶性儿茶素,但是酰化反应发生的位点有所不同,总的来说酰基供体碳链长度一般大于等于8;酶法主要利用脂肪酶使脂肪酸或脂肪酸酯通过直接酯化或酯交换反应制备脂溶性儿茶素,在儿茶素及其化合物上键合烷基,由此提高了儿茶素类化合物在油脂体系中的溶解度,拓展了其应用领域。     

茶多酚制品中有机溶剂多残留检测方法研究进展

现有的茶多酚分离制备工艺大多采用有机溶剂或大孔树脂,给产品带来了有机溶剂残留的安全隐患。本文概述了茶多酚的生产工艺及其残留溶剂的检测方法,主要介绍了直接进样气相色谱法、顶空气相色谱法和固相微萃取气相色谱法这三种方法在残留溶剂测定中的应用进展;提出有必要研究有机溶剂多残留快速检测技术,制定限量标准,提升加工工艺。     

Effect of electrolyzed reduced water on chiral theanine and polyphenols in tea

Pure d/l-theanine enantiomers were synthesized separately, and SEM was used for their crystal-structure observation. The novel enantiomeric separating method by HPLC was established using the chiral selector of β-CD in the mobile phase. Green tea, white tea, oolong tea, black tea and Pu-erh tea were tested for theanine enantiomers by different degrees of fermentation. The significantly higher d-enantiomeric proportion of theanine was found in white tea than the others, which was probably due to its specific processing step of withering. The effect of electrolyzed reduced water (ERW) on enantiomeric theanine and polyphenols in tea was explored. There was no change of theanine, but rather a loss of ECG (epicatechin gallate) and an increasing amount of GA (gallic acid). ERW also reduced tea cream, which contains significant amount of polyphenols, indicating its potential application in the tea-beverage industry.     

茶叶之路沿途晋商会馆设立及作用与分布特征

根据茶叶之路的线路及特征，通过对茶叶之路沿途所建的晋商会馆的介绍，探讨了茶叶之路沿途晋商会馆的设立及作用，并总结了晋商会馆的分布特征，对茶叶之路的发展起到了推动作用。     

金华市婺城区发展茶业循环经济的路径分析

本文阐述循环经济的理念在茶业生产中的应用。资源节约利用,产业持续发展和环境保护是区别传统茶业生产与循环农业模式的主要因素。金华市婺城区良好的气候条件、良种茶园的建设成果、茶叶生产加工机械化的普及改良和茶业标准化、组织化建设为发展茶业循环经济创造了有利的条件,但传统茶业生产根源的广泛性和循环经济意识的缺乏、及相应的支撑体系不完善也制约了茶业循环经济的发展。加大宣传,提高循环经济意识,加强循环茶业技术支撑和成熟模式的示范推广,完善保障体系,是推动茶业循环经济发展的重要途径。     

The antioxidant activity of teas measured by the FRAP method adapted to the FIA system: Optimising the conditions using the response surface methodology

This study proposes a FRAP assay adapted to FIA system with a merging zones configuration. The FIA system conditions were optimised with the response surface methodology using the central composite rotatable design. The optimisation parameters studied were: the carrier flow rate, the lengths of the sample and reagent loops, and reactor length. The conditions selected in accordance with the results were: carrier flow rate of 1.00 ml/min, length of the loops 18.2 cm and length of the reaction coil 210.1 cm. The detection and quantification limits were, respectively, 28.6 and 86.8 μmol/l Fe²⁺, and the precision was 1.27%. The proposed method had an analytical frequency of 30 samples/h and about 95% less volume of FRAP reagent was consumed. The FRAP assay adapted to the FIA system under the optimised conditions was utilised to determine the antioxidant activity of tea samples.     

电力系统动态消息编码技术

为方便通信双方在未协商通信内容情况下动态、高效地交换模型和数据,提出了电力系统动态消息编码技术。该编码技术采用二进制自描述方式描述大型电力系统模型和数据,共有四种编码方式:M0用于兼容抽象语法标记(ASN.1);M1在ASN.1的基础上加入名字选项,用于少量数据的结构化描述;M2扩展编码头部,编码效率高;M3扩展编码头部,描述类信息。实际使用中,首次通信时使用M3发送类型和结构信息,其后通信使用M2发送数据;当类型和结构发生变化时,再次发送类型和结构信息,有效地解决了数据量和维护的矛盾问题。     

向筱原一男学习 访谈：长谷川豪&柳亦春&郭屹民

通过长谷川豪、柳亦春和郭屹民三人的对谈,从贯穿筱原一男整个创作生涯的“传统”、“抽象”等关键词出发,通过对谈进而对“构成”、“即物性”等方面的展开,深入讨论了筱原一男作品中对于国内读者而言晦涩难懂的阅读障碍。同时也对诸如教育、海外影响以及对日本当代建筑的意义等方面触及,试图更加具有针对性地来呈现筱原一男作品的深度及其意义。