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R.K. Singh R.K. Sharma A.K. Singh V.P. Singh N.K. Singh S.P. Tiwari T. Mohapatra 《Euphytica》2004,135(2):135-143
At present, testing for distinctness, uniformity and stability (DUS) of crop varieties relies on a set of morphological characters. These characters suffer fromthe limitations of number, interaction with the environment in which the variety grows and subjectivity in decision-making. The potential of DNA-based markers such as sequence tagged microsatellite site (STMS), for establishing DUS merits investigation. In the present study, a set of 55 mapped STMS markers, selected from 12 linkage groups of rice genome, was used to examine distinctness of 23 aromatic rice genotypes including the commercially important Basmati varieties. Forty-one of these markers (74.5%) showed polymorphism between the varieties. The number of alleles per locus ranged from 2–4 with an average of 2.3. The polymorphism information content (PIC) of the markers varied from 0.083 to 0.665 with an average of 0.338. All the varieties could be differentiated from each other at a low probability (0.07×10-13) of identical match by chance. The marker-based clustering of the varieties corresponded with the known phenotypic classification, thereby providing confidence in the distinctness established by the mapped STMS markers. The utility of these markers to study uniformity and stability was analysed using a commercially important crossbred Basmati rice variety Pusa Basmati 1(IET-10364) that contributes about 40–50% of Basmati rice export from India. Genotyping of twenty individual plants, grown from the nucleus, breeder, foundation, certified and farmer's saved seed samples using all the 55 markers revealed no variation among the plants. These observations suggested that the set of mapped markers employed in this study could be further used for establishing distinctness of aromatic rice varieties and for studying DUS of the important commercial variety Pusa Basmati 1. This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Interactions between grazing animals and vegetation are assessed from three temporal perspectives: millions, thousands and hundreds of years. Data abundance and quality are highest for recent time periods, but geological data provide a background to the understanding of present-day grazing–vegetation interactions. The Quaternary glaciations and recent anthropogenic influences have contributed to the loss of European mega-herbivores. The geological record from the Eemian interglacial in Denmark suggests that presence of elephant and rhinoceros did not create widespread openings in forest cover. Large populations of giant deer in Ireland became extinct 11 000 years ago. We propose a theory that the giant deer were sufficiently abundant to convert juniper scrub communities into open grassland at a regional scale. The balance between grazers and browsers has undergone continuous change during the last 10 000 years with significant consequences for forest composition and structure. Hunting statistics and archival records permit crude reconstructions of population dynamics for certain ungulate species. High resolution pollen analysis and long-term monitoring generate reconstructions of vegetation that can be compared with fluctuating grazing pressure during the last few hundred years. Such data can be used to validate simulation models of grazing–vegetation interactions. 相似文献
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Adam C.D. Barlow Md. Ishtiaq U. Ahmed Md. Mizanur Rahman 《Biological conservation》2008,141(8):2032-2040
Baseline data on distribution and abundance of tigers in the Sundarbans is required to identify problem areas and evaluate management strategies. This paper outlines a khal (creek) bank survey of track set frequency throughout the Bangladesh Sundarbans to aid formulation of a management-driven monitoring program. Three teams of two observers surveyed a total of 1 201 km of waterways throughout the Sundarbans, recording 1 338 tiger track sets. These sets became unrecognizable as tiger sign after a mean 10 days (range 6-14). Proportion of detectable sign recorded was 0.91. Mean (±standard error) sample unit track frequency was 1.12 ± 0.86 track sets/km of khal. The mean coefficient of variance in sample unit track rate, estimated by multiple counts of six sample units, was 0.21 (range 0.06-0.34). Track frequency generally increased from northeast to southwest. Four sample units (6%) had signs of reproduction, with a mean litter size of 1.75 ± 0.5. Monte Carlo simulation suggests a monitoring program of one complete survey every two years will have power of 0.8 (α = 0.2), to detect track frequency declines of ?19% and increases of ?17%. We recommend this monitoring scheme be implemented on the Indian side of the Sundarbans to provide a standard assessment of the tiger population and to form the basis for setting management objectives and evaluating transboundary conservation initiatives. 相似文献
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Vassilis M Papadakis Alexis Glaropoulos Marsela Alvanopoulou Maroudio Kentouri 《Aquaculture Research》2016,47(12):4015-4023
Dominance is a strong behaviour exhibited by farmed species that very often impinges on fish growth and welfare. This study presents a behavioural approach of dominance, where colour pattern differentiation was tested as a signal of dominance presence in small sea bream population. The phenotype of dominance was first described in detail, referring to vertical dark stripes and splayed fins. Fish were kept in triplicate tanks for 21 days, during which they were exposed to three feeding conditions (well fed, limited fed and no‐fed) and continuous video recordings. Each tank was stocked with 15 individuals (~30.34 ± 1.70 g), and they were confined to the half volume of the tank via a removable net pen. Percentage of dominants per population was found up to 40%, while duration was calculated to 53.52 ± 7.44 s. Dominance behaviour was further quantified via colour pattern differentiation on sea bream body (CDA, contrast of dominance appearance). The results demonstrated that the body colour of sea bream is directly linked to species social hierarchies and such variations are visual signal of dominance rank inside the population. The fish feeding conditions had an influence on the dominance presence, but not on the dominance rank (as measured by the CDA) between fish groups. The described study provides state‐of‐the‐art knowledge on sea bream dominance. In relevance to aquaculture, dominance quantification would be a reliable tool to evaluate non‐equal food distribution in tank‐held populations, thus, avoid non‐regulate growth performance of fish before transfer to sea‐cage installations. 相似文献
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Jawahar Lal Katara Ram Lakhan Verma Debkanta Nayak Umakanta Ngangkham Soham Ray Hatanath Subudhi Lambodar Behera Sanghamitra Samantaray Ravi Nageswara Rao Onkar Nath Singh Trilochan Mohapatra 《Plant Breeding》2017,136(1):74-82
Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F1s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding. 相似文献
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Urban green infrastructure provides city dwellers numerous benefits. Among them, cultural ecosystem services (CES) are distinguished by being easily perceived and essential for people and their well-being. However, not all CES are equally easy to perceive, resulting with some of the CES categories being weakly explored. Research on CES also rarely considers elements of urban green infrastructure other than parks and forests. Therefore, there is a lack of research on different components of urban green infrastructure, especially tree-based, perceived in relation to CES. This paper presents the results of focus group participatory mapping implemented with citizens in the city districts of Zagreb on the perception of five selected CES categories in various types of urban green infrastructure. Our results show that participants perceived 13 different types of tree-based urban green infrastructure as providers of CES. We also distinguish patterns in the perception of CES categories and their connection with types of tree-based urban green infrastructure. Tree lines are perceived as providers of aesthetical experiences. Furthermore, forests and park forests are perceived in relation to place attachment and recreational activities, while parks are versatile and provide all explored CES. Other types that emerged as important were greenways, greenery around residential buildings and educational institutions, which provokes rethinking of a careful planning of the entire repertoire of urban green infrastructure. 相似文献
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