Psychrotrophic violacein-producing bacteria isolated from Lake Winnipeg,Canada

Violet and purple bacterial pigmentations are uncommon when considering strict aerobes. Here we report discovery of two novel isolates, P102 and P117 from the freshwater Lake Winnipeg, each with violet to deep purple colony colouration. The relationship between pigment production and growth was investigated under different conditions, including a variety of carbon sources, pH, and temperatures. The violet compounds were purified using polar organic solvents, and then structurally characterized via mass spectrometry and nuclear magnetic resonance to be violacein and deoxyviolacein. Strain P117 produced higher concentrations of the darker pigment deoxyviolacein, which resulted in a deeper hue of violet than in cultures of P102. Strain P102, synthesized violacein maximally in liquid rich organic medium at pH 8 and 20 °C, and had 99.3% sequence similarity to the 16S rDNA from Janthinobacterium lividum. Strain P117, related to Massilia violaceinigra sharing 99.2% 16S rDNA sequence similarity, also produced violacein at similar optimal conditions, but developed higher concentrations of pigment at 15 °C. Culture-based methods found that violacein producers composed 0.001% or 0.023% CFU of environmental heterotrophic populations using BG-11 and potato-based media, respectively. Culture-independent high-throughput ribosomal 16S V4 sequencing of environmental DNA was used to detect Gram-negative species known for their production of violacein. Of all bacterial and archaeal sequences present during the fall in littoral waters and sediment of Lake Winnipeg, 5.5% and 6.3%, respectively, belonged to species that have similar pigment, demonstrating the scarcity of violacein producers in this aquatic freshwater system.     

大凌河流域典型水生植物微生物群落吸附特征试验分析

本文采用高通测序方法对大凌河流域五刺金鱼藻、格菱、狐尾藻、水蓼及芦苇5种水生植物不同季节的微生物群落进行试验分析,分析表明:藻类密度在沉水植物表层要高于浮叶植物表层,微生物群落在浮叶植物根部的结构与其表面存在明显差异。各水生植物的优势生物群落依次为变形菌、梭菌、柔膜菌、鞭脂杆菌、杆菌、疣微菌门,且各微生物群落在季节丰度上存在明显差异性。由此可见,水环境对生物群落的影响要高于植物类型。研究成果可为大凌河流域生态修复工程提供重要理论依据。     

Personalizing 3D virtual fashion stores: Exploring modularity with a typology of atmospherics based on user input

This research develops a typology of atmospherics that contains user-identified modules and modular options for personalizing 3D virtual fashion stores. A content analysis of 46 focus group discussions (n = 170) was conducted to understand the user’s perspective for personalizing 3D virtual fashion store atmospherics. Based on three atmospheric categories (pathfinding assistant, environment, and the manner of product presentation), 17 modules and 207 modular options were identified for personalizing 3D virtual stores. This research pioneers the development of an atmospherics typology for personalizing 3D virtual shopping environments as a persuasive selling tool in the emerging field of 3D virtual reality (VR) retailing.     

基于TLM2．0的硬件虚拟平台设计

利用OVP（Open Virtual Platform）提供的模型创建基于TLM2．0的硬件虚拟平台,可以为嵌入式软件提供一个快速有效的开发环境,实现软件和硬件的协同设计。提早开发出的软件也可以对系统虚拟平台进行功能验证,分析系统性能,确定系统的最优架构。     

Supporting inconsistent rules in database systems

When a set of rules generates (conflicting) values for a virtual attribute of some tuple, the system must resolve the inconsistency and decide on a unique value that is assigned to that attribute. In most current systems, the conflict is resolved based on criteria that choose one of the rules in the conflicting set and use the value that it generated. There are several applications, however, where inconsistencies of the above form arise, whose semantics demand a different form of resolution. We propose a general framework for the study of the conflict resolution problem, and suggest a variety of resolution criteria, which collectively subsume all previously known solutions. With several new criteria being introduced, the semantics of several applications are captured more accurately than in the past. We discuss how conflict resolution criteria can be specified at the schema or the rule-module level. Finally, we suggest some implementation techniques based on rule indexing, which allow conflicts to be resolved efficiently at compile time, so that at run time only a single rule is processed.An earlier version of this work appeared under the title Conflict Resolution of Rules Assigning Values to Virtual Attributes inProceedings of the 1989 ACM-Sigmod Conference, Portland, OR, June 1989, pp. 205–214.Partially supported by the National Science Foundation under Grant IRI-9157368 (PYI Award) and by grants from DEC, HP, and AT&T.Partially supported by the National Science Foundation under Grant IRI-9057573 (PYI Award), IBM, DEC, and the University of Maryland Institute for Advanced Computer Studies (UMIACS).     

三维虚拟仿真产品展示平台的应用与研究

随着网络的迅速发展,网上购物越来越受到人们的青睐。顾客希望能从网站上更详细地了解商品外观和体验功能,因此如何更好地向消费者展示商品是电子商务中吸引用户需要解决的问题。本文所讨论的三维虚拟仿真产品展示平台会给使用者提供一个虚拟仿真的环境,使用户“足不出户”就能有真实的体验,满足用户的浏览需求。     

基于Virtools的虚拟现实技术及在特种设备教学中的应用

本文以电梯为研究对象,运用虚拟现实技术建立电梯虚拟样机,开发了一种电梯零部件识别系统用于电梯司机的考核。现行的考核方式存在过程繁琐、成绩统计困难等问题,就这些问题提出了合理的解决方案,并将方案应用于开发的系统中。该系统是虚拟现实技术在特种设备领域的全新应用,构建的虚拟电梯完整地呈现了真实的电梯,而且可以从多角度观察其结构的每个细节,方便教学与考核。系统结合SolidWorks、3Dmax、Virtools等软件,以触摸选择为主要的操作方式,具有优良的操作性与交互性,且投资少、开发周期短。     

基于数据融合的ABC-SVM社区疾病预测方法

针对现有社区医疗服务中的疾病预测方法存在数据利用率低、疾病分析类型单一、自动化程度差、疾病预测效果不理想等不足,提出在物联网大数据环境下可用于社区医疗的健康数据融合及疾病预测方法. 通过主成分分析(PCA)和聚类分析对社区中居民的生理指标数据进行特征提取;结合人工蜂群(ABC)算法构造支持向量机(SVM)非线性分类器对数据进行特征级融合分析并预测潜在疾病. 实验结果表明,所提方法的疾病识别准确率达到93.10%,相较于传统SVM方法和BP神经网络方法分别提高17.24% 和72.41%. 该方法能够在提高数据利用率、降低计算资源消耗的前提下有效识别多种潜在疾病,可实现疾病早发现、早预防、早治疗;可广泛应用于社区健康管理、老年社区监护甚至临床医疗.     

高温混合障碍空间中的移动机器人路径规划

为了解决高温场景中移动机器人全局路径规划所面临的安全与效率问题,提出高温热源虚拟障碍的定义,建立混合障碍空间模型,将高温场景中的路径规划问题转化为高温混合障碍空间中考虑路径温度代价和长度代价的多目标优化问题. 改进NSGA-Ⅱ算法,通过选取优秀非可行解扩展种群,提高了种群多样性和进化效率,提出新的交叉和变异概率计算方法. 根据种群进化进程和个体代价函数值调整概率,实现了种群前期搜索能力和后期收敛性的平衡. 仿真所得的最优路径结果表明,该改进算法的路径长度代价虽然比原算法和其他改进算法略有增加,但温度代价大幅降低,更有效地避免了陷入局部最优.     

图书馆促进社会包容性发展的思考

促进社会包容性发展是图书馆从诞生伊始就坚守的价值观,通过保障信息自由与信息公平、促进多元文化理解、提高公民媒介信息素养等途径,图书馆致力于支持和促进社会包容性发展。图书馆需要进一步强化服务意识、加强跨组织协同、切实发挥信息素养培养及社区教育的服务职能、促进社区参与,为社会包容性发展提供更大支持。