棘豆属刺垫棘豆亚属(豆科)的订正

1971年,N.Ulzijkhutag根据采自阿拉善戈壁(=Bordzon-Go-bi)的标本(1970-07-29,V.I.Grubov,N.Ulzijkhutag et G.Tserendalzhid,无号)发表新种Oxytrops grubovii,并以此建立新组Oxytropis subgen.Traganthoxytropis sect.Monan-thos;1987年,G.P.Yakovlev将O.grubovii归入海绵豆属Spongiocarpella;1988年,张振万同意N.Ulzijkhutag的意见,并根据刺垫棘豆之龙骨瓣无喙等特征将Ulzijkhutag的刺     

长臂生物素对硝基苯酯的合成

以羰基二咪唑为催化剂,生物素与6－氨基己酸甲酯反应生成生物素－6－氨基己酸甲酯,该化合物通过酸碱萃取与原料分离,皂化后生成生物素氨基己酸．生物素氨基己酸在吡啶存在下与三氟乙酸对硝基苯酯进行转酯反应即得生物素对硝基苯酯．最后经光谱、色谱及核酸杂交证实了长臂生物素对硝基苯酯的化学结构和生物学活性．     

Mechanisms of increasing the intensity of ultraviolet fluorescence of irradiated cells

As shown on the in vitro irradiated Ehrlich ascites tumor cells the increase in the intensity of ultraviolet fluorescence (UVF) of the exposed cells occurs only in such incubation conditions when an increase in the tryptophan content of irradiated cells becomes possible. The increase in the UVF intensity and in the tryptophan content are quantitatively identical. It is concluded that the increase in the content of the fluorescing substance, tryptophan, in the irradiated cells is the cause of the increase in the UVF intensity.     

去传入神经引起大鼠延髓后角P物质、胆囊收缩素、神经降压肽、亮-脑啡肽变化机制的探讨

去传入神经可引起后角一些肽类变化和含肽的大颗粒小泡以胞吐的形式在非突触部位释放。为了探讨这一作用机制,本文切除一侧刚髭部皮肤,应用免疫组化方法观察并定量分析术后不同时间延髓后角浅层SP、CCK、NT、L-ENK阳性纤维的变化及利多卡因对后角浅层SP的影响。结果显示:术后1小时,延髓后角浅层SP损伤侧较对照侧升高,第3～7天未见显著性减少或升高,第8天开始脱失(P<0.05),第14天脱失更甚,第30天开始恢复,第90～120天基本恢复。CCK变化基本同SP。NT、L-ENK术后不同时间(7～120天),两侧比较未见显著性差异(P>0.05)。应用和多卡因后,后角浅层SP损伤侧较对照侧未见明显减少(P>0.05)。根据上述结果,本文推测:去传入神经导致延髓后角浅层神经肽的变化,可能是神经损伤区(神经瘤或再生芽)产生异常电活动,引起延髓后角浅层神经肽释放的结果。     

用于聚合酶链式反应(PCR)引物设计的计算机程序

 本文报道了两个用于PCR引物设计的计算机程序PCRDESN和PCRDESNA。PCRDESN程序主要从以下4个方面评价用户自己设计的一对引物的质量:(1)引物内的碱基反向重复或发夹结构,(2)两个引物之间的碱基互补配对,(3)两个引物之间的同源性,(4)引物的碱基组成及特点和T_m值计算。通过用多例文献发表的及本院有关实验室提供的引物对序列的验证,确定了程序的运算参数,证明该程序能较好地检验引物对的质量和解释某些PCR实验失败的原因。PCRDESNA程序采用逐级优化的方法和比PCRDESN所选用的更严紧的引物选择参数对用户提供的核酸序列进行快速检索,以确定所有可能的和合适的引物对。     

Corticostatic peptides.

In the last four years corticostatic (anti-ACTH) peptides have been isolated from human, rabbit, guinea pig and rat tissues. These peptides do not act via the cAMP cell signalling system but rather via the inhibition of the binding of ACTH to its receptor most probably through direct competition with the 14-18 sequence of ACTH for receptor binding. ACTH has specific high affinity receptors on adrenal cells but rabbit corticostatin I (CSI) has high capacity, low affinity receptors which are competed for by unlabelled excess CSI but not by excess ACTH. This indicates the presence of specific CSI adrenal cell receptors. The rabbit pituitary, hypothalamus, thalamus, adrenals, lungs and placenta contain sizeable amounts of immunoassayable CSI. Immunochemical localization of CSI indicates that it is present in the large macrophages and in neutrophils in rabbit lung, in macrophages and "supporting" endothelial cells in the spleen and in the adrenals in the cells of the zona reticularis. We have also isolated and identified new peptides which contain 12 cysteines from immune cells of humans, rats and a teleost, the carp. The functions of these peptides are now being determined. This large family of peptides may have many other, yet unidentified functions but at present we can only describe a small number of these.     

草鱼血液学的研究：Ⅱ.血清电解质和尿素氮的周年变化

本文研究了草鱼稚鱼血清钾、钠、钙、氯、无机磷和尿素氮含量的周年变动规律,分析了水温、体重对上述血液化学成分的影响。结果显示其周年变动无一定规律性可循。血清钠、氯变动辐度较小,无机磷和尿素氮变动幅度较大,幅值约有4倍。水温与血清钾显示正相关(P≤0.05),与钠、钙显示负相关(P≤0.01,P≤0.05)。血清钠、尿素氮有随体重增加含量增高的趋势,而钾却显著减少。     

从田菁胶工业下脚料生产田菁蛋白胨的研究——[Ⅰ]田菁蛋白胨化学成份的研究

本文首次研究了田菁蛋白胨一般化学成份、氨基酸组份、肽链平均长度、微量元素、维生素以及红外、紫外光谱等。本文还将田菁蛋白胨氨基酸、红外光谱等和英国大豆蛋白胨作了比较。研究结果附图表于后。