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991.
Yosuke Kato Mitsunori Iwase Hiroaki Kanazawa Natsuki Kawata Yukie Yoshimori Katsunori Hashimoto Toyoharu Yokoi Akiko Noda Kenzo Takagi Yasuo Koike Takao Nishizawa Masahiko Nishimura Mitsuhiro Yokota 《Experimental Animals》2003,52(4):285-294
The present study aimed to evaluate the development of pulmonary hypertension by serial echocardiography, including measurements of pulmonary artery (PA) flow velocities, and correlate echocardiographic indices with pathological findings in rats administered monocrotaline (MCT). MCT (60 mg/kg body weight) or physiologic saline was administered to a total of 9 male Wistar rats at the age of 4 weeks (MCT group: n = 4, control group: n = 5, respectively). Echocardiography was performed serially until the age of 8 weeks. The ratio of right ventricular (RV) outflow tract dimensions to aortic dimensions increased progressively in the MCT group and became significantly greater than that of the control group after the age of 6 weeks. Peak PA velocity (Peak V) in the MCT group was significantly less than that of the control group at the ages of 7 and 8 weeks. The ratio of acceleration time to ejection time (AT/ET) in PA flow waveforms declined progressively and was significantly less than that of the control group after the age of 6 weeks. The ratio of RV weight to body weight (RVW/BW) in the MCT group was significantly greater than that of the control group. Both AT/ET ratio and Peak V were significantly inversely correlated with RVW/BW ratio. Furthermore, these echocardiographic findings were also significantly inversely correlated with the mean cross-sectional RV myocyte area. In conclusion, the progressive development of pulmonary hypertension leading to RV hypertrophy can be evaluated appropriately by echocardiography including PA flow Doppler indices in rats. 相似文献
992.
Yasukazu Nakamura Takakazu Kaneko Shusei Sato Masahiko Ikeuchi Hiroshi Katoh Shigemi Sasamoto Akiko Watanabe Mayumi Iriguchi Kumiko Kawashima Takaharu Kimura Yoshie Kishida Chiaki Kiyokawa Mitsuyo Kohara Midori Matsumoto Ai Matsuno Naomi Nakazaki Sayaka Shimpo Masako Sugimoto Chie Takeuchi Manabu Yamada Satoshi Tabata 《DNA research》2002,9(4):135-148
993.
Manabu Shibasaki Thad E Wilson Jian Cui Craig G Crandall 《Journal of applied physiology》2002,93(6):1947-1951
Nitric oxide (NO) contributes to active cutaneous vasodilation during a heat stress in humans. Given that acetylcholine is released from cholinergic nerves during whole body heating, coupled with evidence that acetylcholine causes vasodilation via NO mechanisms, it is possible that release of acetylcholine in the dermal space contributes to cutaneous vasodilation during a heat stress. To test this hypothesis, in seven subjects skin blood flow (SkBF) and sweat rate were simultaneously monitored over three microdialysis membranes placed in the dermal space of dorsal forearm skin. One membrane was perfused with the acetylcholinesterase inhibitor neostigmine (10 microM), the second membrane was perfused with the NO synthase inhibitor N(G)-nitro-l-arginine methyl ester (l-NAME; 10 mM) dissolved in the aforementioned neostigmine solution (l-NAME(Neo)), and the third membrane was perfused with Ringer solution as a control site. Each subject was exposed to approximately 20 min of whole body heating via a water-perfused suit, which increased mean body temperature from 36.4 +/- 0.1 to 37.5 +/- 0.1 degrees C (P < 0.05). After the heat stress, SkBF at each site was normalized to its maximum value, identified by administration of 28 mM sodium nitroprusside. Mean body temperature threshold for cutaneous vasodilation was significantly lower at the neostigmine-treated site relative to the other sites (neostigmine: 36.6 +/- 0.1 degrees C, l-NAME(Neo): 37.1 +/- 0.1 degrees C, control: 36.9 +/- 0.1 degrees C), whereas no significant threshold difference was observed between the l-NAME(Neo)-treated and control sites. At the end of the heat stress, SkBF was not different between the neostigmine-treated and control sites, whereas SkBF at the l-NAME(Neo)-treated site was significantly lower than the other sites. These results suggest that acetylcholine released from cholinergic nerves is capable of modulating cutaneous vasodilation via NO synthase mechanisms early in the heat stress but not after substantial cutaneous vasodilation. 相似文献
994.
Mitsutoshi Kitao Laiye Qu Takayoshi Koike Hiroyuki Tobita Yutaka Maruyama 《Physiologia plantarum》2004,122(2):226-232
The seasonal changes in photosynthetic properties in 1-year-old needles of Sakhalin spruce ( Picea glehnii ) were measured using the chlorophyll fluorescence technique at various temperatures (5, 10, 20, 25 and 30°C). In the course of seasonal change, a temporary decrease in the quantum yield of PSII electron transport (ΦPSII ) was observed just before budbreak. A decline in photochemical quenching ( q P ) was observed at the same time as that of ΦPSII but only at the two lowest temperatures (5 and 10°C). Photochemical efficiency of open PSII ( F v '/ F m ') also declined just before budbreak at 25 and 30°C. An increase in thermal energy dissipation as indicated by a decrease in F v '/ F m ' before budbreak was not significant at lower temperatures (5 and 10°C) in spite of the declines in q P . This implies that thermal energy dissipation necessitated by the decline in ΦPSII might not be sufficiently strong to prevent a decline in q P at lower temperatures. On the other hand, at higher temperatures no decline was observed in q P because ΦPSII decreased to a relatively small extent, therefore thermal energy dissipation is sufficient in coping with the excessive energy accumulation in PSII. Seedlings of Sakhalin spruce exposed to ambient air temperature below 10°C before budbreak exhibited photoinhibition indicated by a decrease in the maximal photochemical efficiency of PSII ( F v / F m ) after an overnight dark adaptation. The present study suggests that 1-year-old shoots of Sakhalin spruce have an increased susceptibility to photoinhibition at low temperature just before budbreak. 相似文献
995.
Purification of inactivated photoresponsive nitrile hydratase 总被引:2,自引:0,他引:2
T Nagamune H Kurata M Hirata J Honda H Koike M Ikeuchi Y Inoue A Hirata I Endo 《Biochemical and biophysical research communications》1990,168(2):437-442
Photoresponsive nitrile hydratase from Rhodococcus sp. N-771 was purified in its inactivated form. The enzyme had a molecular weight of approximately 60 kDa and consisted of 2 subunits each having molecular weight of 27.5 and 28 kDa. The enzyme also contained 2 iron atoms/enzyme as a cofactor. The enzyme was more stable in its inactivated form, rather than the activated during storage in the dark. The enzyme was most stable in the temperature region of 0-35 degrees C, and lost its activity above 40 degrees C. The enzyme was most stable in the pH region of 6-8. The optimum temperature and pH for the enzyme activity was 30 degrees C and 7.8, respectively. The enzyme showed wide substrate specificity, and most of the metal ions did not affect enzyme activity significantly. The absorption spectrum revealed the presence of some cofactor which changed form after photoirradiation. 相似文献
996.
997.
Caffeine promotes survival of cultured sympathetic neurons deprived of nerve growth factor through a cAMP-dependent mechanism. 总被引:3,自引:0,他引:3
The effects of caffeine on neuronal survival independent of trophic factor support were examined in developing superior cervical ganglion in vitro. We found that caffeine promoted neuronal survival in the absence of nerve growth-factor (NGF) in a dose-dependent manner (EC50 = 6 mM). Pulse treatment with caffeine or high K+ (40 mM), which caused only a transient increase in intracellular free Ca2+ levels ([Ca2+]i), did not promote survival. In contrast, caffeine potentiated the saving effect of various phosphodiesterase inhibitors including theophylline (EC50 = 3 mM) and 3-isobutyl-1-methylxanthine (EC50 = 0.4 mM). Non-xanthine phosphodiesterase inhibitor Ro 20-1724 potentiated the survival promoting effect of caffeine or IBMX. Indeed, administration of 20 mM caffeine rapidly restored the cAMP level of NGF-deprived neurons to normal (0.34 pmol/well) within 10 min; the level reached a plateau level (0.69 pmol/well) at 10 h. Even after 1 day, the sustained level was maintained in the presence of caffeine. In contrast, noradrenaline and isoproterenol, which cause only a transient increase in cAMP levels, did not support survival. These data, in conjunction with others, suggest that sustained levels of second messengers, including not only the [Ca2+]i but also the cAMP level, would support the survival of superior cervical ganglion cells independent of trophic factor support. 相似文献
998.
Y Hashimoto M Kawamura K Ichikawa T Suzuki T Sumida S Yoshida E Matsuura S Ikehara T Koike 《Journal of immunology (Baltimore, Md. : 1950)》1992,149(3):1063-1068
NZW x BXSB F1 (W/B F1) male mice develop systemic lupus-like disease, and several autoantibodies, circulating immune complexes, and lupus nephritis become apparent. The abnormally high incidence of degenerative coronary vascular disease with myocardial infarction and thrombocytopenia due to the presence of both platelet-associated antibodies and circulating antiplatelet antibodies in this animal has been reported. We found that W/B F1 male mice produced autoantibodies against cardiolipin (aCL) and that the titer of aCL increases with age. aCL from W/B F1 male mice were mainly IgG and binding activity to cardiolipin was aCL-cofactor (beta 2-glycoprotein I (beta 2-GPI)) dependent. We developed monoclonal aCL from these animals and examined specificity of the autoantibodies. All the mAb used reacted with the negatively charged phospholipids, cardiolipin, phosphatidylserine, and phosphatidylinositol, and some reacted with platelets and DNA. The addition of human or mouse beta 2-GPI enhanced the titer for monoclonal aCL from the W/B F1 mice. From the results of competitive inhibition enzyme immunoassay with monoclonal aCL and purified beta 2-GPI, aCL from the W/B F1 mice recognized the complex of CL and beta 2-GPI. The W/B F1 male mouse may be an appropriate model for use in studies on the pathologic significance of aCL in patients with antiphospholipid syndrome. 相似文献
999.
M Takagi T Nakahata T Kubo M Shiohara K Koike A Miyajima K Arai S Nishikawa K M Zsebo A Komiyama 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(11):3446-3453
The proliferative capacity of mouse connective tissue-type mast cells (CTMC) was analyzed by using a newly discovered c-kit ligand, termed stem cell factor (SCF). More than 90% of CTMC in the peritoneal cavity responded to recombinant rat SCF (rrSCF) and were able to give rise to pure mast cell colonies in methylcellulose culture. Serial observation (mapping) of growth of individual CTMC in culture containing rrSCF confirmed their striking proliferative ability. No serum but accessory cells (non-CTMC cells) in the peritoneal population were required for the clonal growth of CTMC induced by rrSCF in our methylcellulose culture of whole peritoneal cells. The rrSCF-induced mast cell colony formation from peritoneal CTMC was completely inhibited by the addition of anti-c-kit antibody, which can block the binding of SCF to c-kit, to the culture. When IL-3 was combined with rrSCF, mast cell colonies dramatically increased in size. Mapping studies revealed that the combination of the two factors augmented the proliferative rate of CTMC. Approximately 60% of the constituent cells of the mast cell colonies which were formed from peritoneal CTMC in the culture containing rrSCF alone were stained with berberine sulfate, which is a characteristic of CTMC. However, most mast cells which were induced by rrSCF+IL-3 from peritoneal CTMC contained berberine(-)-safranin(-)-Alcian blue(+) granules. Although IL-4 exhibited little synergism with rrSCF in the induction of CTMC proliferation, the addition of IL-4 to the culture containing rrSCF+IL-3 resulted in an increase in mast cells which retained CTMC characteristics. 相似文献
1000.
Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor. 总被引:24,自引:0,他引:24
E Matsuura Y Igarashi M Fujimoto K Ichikawa T Suzuki T Sumida T Yasuda T Koike 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(12):3885-3891
Anticardiolipin antibodies (aCL) found in sera from patients with SLE react with cardiolipin (CL) in the presence of a 50-kDa serum cofactor. The cofactor, which was identified to be beta 2-glycoprotein I by sequencing the N-terminal amino acids, not only enhances CL binding by antibodies in SLE but also depresses it by antibodies associated with syphilis. Cofactor-dependent binding of aCL in SLE to solid phase CL was competitively inhibited by the simultaneous addition of fluid phase CL but was unaffected by either prior or simultaneous addition of a high excess of the cofactor. Binding of aCL in syphilis to solid phase CL was competitively inhibited by either addition of the cofactor or fluid phase CL. aCL in SLE reacted with CL, PS, and PA in the presence of cofactor. In contrast, biotinyl-cofactor bound directly to these anionic phospholipids (PL) and also to PG. These results show that the cofactor-CL complex bears an epitope that confers recognition specificity for aCL in SLE, in contrast with direct CL recognition by syphilitic aCL. The direct binding of the cofactor to PL suggests that the cofactor dependence of aCL binding to PL is due to recognition by aCL of a unique epitope generated upon the formation of the cofactor-CL complex. 相似文献