Raddeanin A induces human gastric cancer cells apoptosis and inhibits their invasion in vitro

Raddeanin A is one of the triterpenoid saponins in herbal medicine Anemone raddeana Regel which was reported to suppress the growth of liver and lung cancer cells. However, little was known about its effect on gastric cancer (GC) cells. This study aimed to investigate its inhibitory effect on three kinds of different differentiation stage GC cells (BGC-823, SGC-7901 and MKN-28) in vitro and the possible mechanisms. Proliferation assay and flow cytometry demonstrated Raddeanin A’s dose-dependent inhibitory effect and determined its induction of cells apoptosis, respectively. Transwell assay, wounding heal assay and cell matrix adhesion assay showed that Raddeanin A significantly inhibited the abilities of the invasion, migration and adhesion of the BGC-823 cells. Moreover, quantitative real time PCR and Western blot analysis found that Raddeanin A increased Bax expression while reduced Bcl-2, Bcl-xL and Survivin expressions and significantly activated caspase-3, caspase-8, caspase-9 and poly-ADP ribose polymerase (PARP). Besides, Raddeanin A could also up-regulate the expression of reversion inducing cysteine rich protein with Kazal motifs (RECK), E-cadherin (E-cad) and down-regulate the expression of matrix metalloproteinases-2 (MMP-2), MMP-9, MMP-14 and Rhoc. In conclusion, Raddeanin A inhibits proliferation of human GC cells, induces their apoptosis and inhibits the abilities of invasion, migration and adhesion, exhibiting potential to become antitumor drug.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

红火蚁及其他两种蚂蚁毒腺细菌鉴定与多样性分析(英文)

【目的】本研究旨在分析红火蚁Solenopsis invicta毒腺细菌群落多样性,并与热带火蚁Solenopsis geminata和聚纹双刺猛蚁Diacamma rugosum比较毒腺细菌群落差异。【方法】采用Illumine Hiseq 2500测序平台对红火蚁(工蚁、有翅蚁和蚁后)、热带火蚁(工蚁)及聚纹双刺猛蚁(工蚁)毒腺细菌群落16S rRNA基因V3-V4区测序,基于测序数据进行生物信息学分析。【结果】变形菌门(Proteobacteria)在红火蚁工蚁、有翅蚁和蚁后以及热带火蚁工蚁毒腺中占优势,而厚壁菌门(Firmicutes)在聚纹双刺猛蚁工蚁毒腺中占优势。与红火蚁工蚁和有翅蚁相比,柔壁菌门(Tenericutes)在红火蚁蚁后毒腺中更丰富。广州红火蚁蚁后毒腺中假单胞杆菌Pseudomonas相对丰度显著高于其在有翅蚁及工蚁中的。螺原体Spiroplasma相对丰度在热带火蚁毒腺中显著高于在聚纹双刺猛蚁工蚁毒腺中的。毒腺中细菌多样性分析发现,芽孢杆菌属Bacillus和乳酸杆菌属Lactobacillus在广西红火蚁工蚁中的相对丰度显著高于在广州红火蚁工蚁中的。然而,乳酸杆菌属细菌在聚纹双刺猛蚁工蚁毒腺中的相对丰度显著高于广西的热带火蚁工蚁毒腺中的。【结论】毒腺细菌组成和多样性在3种不同种类蚂蚁工蚁和红火蚁不同品级中存在差异。     

刺槐叶瘿蚊成虫行为学特征及性诱效果的研究

本文在室内条件下研究了刺槐叶瘿蚊Obolodiplosis rohiniae(Haldemann)成虫羽化、交尾、产卵行为的时间动态节律以及雌蚊性诱效果.结果表明:雌虫5:00-6:00为羽化高峰期,雄虫1:00-2:00为羽化高峰期,雌虫羽化高峰期期迟于雄虫4 h左右.雌蚊羽化后20min左右即外伸产卵器吸引雄蚊交配...     

长唇羊耳蒜的组织培养与快速繁殖

1植物名称 长唇羊耳蒜（Liparis pauliana Hand.-Mazz.）. 2材料类别 种子。     

Production of monoclonal antibodies against the FimA protein of Porphyromonas gingivalis in Nicotiana benthamiana

Porphyromonas gingivalis, a gram-negative anaerobic oral bacterium, causes periodontal disease by binding to saliva-coated oral surfaces. The FimA protein from P. gingivalis is a crucial pathogenic component of the bacterium and a target for vaccine development against periodontal disease. Complementary DNAs encoding the heavy and light chains of two monoclonal antibodies that bind specifically to the FimA protein were cloned into a plant expression vector under the control of the duplicated Cauliflower Mosaic Virus 35S promoter, and agroinfiltration was used to allow the vectors to infiltrate tobacco plants. The expressions of the heavy and light chains in the leaf tissue were detected using antibodies specific to each antibody chain. Western blot analysis showed the specific binding of the plant-derived monoclonal antibodies to the native FimA protein purified from P. gingivalis. Our finding that plant-derived monoclonal antibodies bound specifically to the native FimA protein indicates that plantderived monoclonal antibodies can protect against P. gingivalis invasion.     

12种杀螨剂对山楂叶螨防效评价及其对天敌塔六点蓟马的影响

通过施用不同类型的杀螨剂对苹果山楂叶螨Tetranychus viennensis和天敌塔六点蓟马Scolothrips takahashii的影响进行分析,结果表明,15%哒螨灵EC(0.4 mL/L)、73%炔螨特EC(0.5 mI/L)表现出较好的速效性和持效性,但是对塔六点蓟马有一定的杀伤作用,240 g/L螺螨酯SC(0.2 mL/L)、5%唑螨酯SC(0.4 mL/L)对山楂叶螨相对防效较好.对塔六点蓟马影响较小,500 s/L四螨嗪SC(0.2 mL/L)速效性较差,但对天敌安全,20%甲氟菊酯EC(0.5 mL/L)对山楂叶螨防治效果差,且对天敌杀伤率始终较高,从而可能造成山楂叶螨的爆发.复配药剂10%阿维·四螨SC(0.67 mL/L)综合了单剂的优点,15.6%阿维·丁醚EC(0.5 mL/L)、6%阿维·哒EC(0.5 mL/L)相对单剂对天敌杀伤率有所降低,1.8%阿维·甲氰EC(0.83 mL/L)则主要表现出单剂甲氰菊酯相同的特点.