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71.
Rudolf Fleischmann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1939,11(4):90-94
Ohne ZusammenfassungKgl. ungarischen Pflanzenzuchtstation Komplt. 相似文献
72.
Rudolf Degkwitz 《Protoplasma》1938,31(1):180-183
Ohne Zusammenfassung 相似文献
73.
Doz. Dr. Rudolf Altevogt 《Zoomorphology》1957,46(1):1-110
Ohne Zusammenfassung 相似文献
74.
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77.
Rudolf H. Braun 《Journal of Ornithology》1956,97(1):41-43
Ohne ZusammenfassungMit 7 Aufnahmen des Verfassers auf den Tafeln V und VI 相似文献
78.
Proviral inactivation of the Npat gene of Mpv 20 mice results in early embryonic arrest. 总被引:3,自引:1,他引:3 下载免费PDF全文
M Di Fruscio H Weiher B C Vanderhyden T Imai T Shiomi T A Hori R Jaenisch D A Gray 《Molecular and cellular biology》1997,17(7):4080-4086
The Mpv 20 transgenic mouse strain was created by infection of embryos with a defective retrovirus. When Mpv 20 heterozygous animals were crossed, no homozygous neonatal mice or midgestation embryos were identified. When embryos from heterozygous crosses were cultured in vitro, approximately one quarter arrested as uncompacted eight-cell embryos, indicating that proviral insertion resulted in a recessive lethal defect whose phenotype was manifest very early in development. Molecular cloning of the Mpv 20 insertion site revealed that the provirus had disrupted the Npat gene, a gene of unknown function, resulting in the production of a truncated Npat mRNA. Expression of the closely linked Atm gene was found to be unaffected by the provirus. 相似文献
79.
Salmain M Fischer-Durand N Cavalier L Rudolf B Zakrzewski J Jaouen G 《Bioconjugate chemistry》2002,13(3):693-698
The preparation of several transition metal-carbonyl tracers of biotin and avidin is described. Multiple labeling of avidin was achieved by acylation of some of its amine-bearing residues with N-succinimidyl 4-pentynoate (dicobalt hexacarbonyl). By varying the initial amount of this complex, protein conjugates with the extent of derivatization of up to 13 were obtained. Biotin was labeled with one (eta5-cyclopentadienyl)manganese tricarbonyl moiety by reaction of biotin hydrazide, whereas multiple labeling was reached by successive conjugation of biotin and N-succinimidyl 4-pentynoate (dicobalt hexacarbonyl) or (eta5-cyclopentadienyl)iron dicarbonyl (eta1-N-maleimidato) to poly-L-lysine or fourth generation Starburst dendrimer. All the conjugates displayed a good to excellent bioaffinity toward their respective counterparts, as measured by competitive enzymatic assays. 相似文献
80.
Highly inducible cell lines derived from mice genetically transmitting the Moloney murine leukemia virus genome. 下载免费PDF全文
Permanent, non-virus-producing cell lines have been established from a mouse embryo carrying an endogenous, genetically transmitted Moloney murine leukemia virus (M-MuLV) genome. These cells carry the M-MuLV genome, as demonstrated by hybridization of cellular DNA to M-MuLV complementary DNA, but do not express it at the levels of virus production, accumulation of intracellular viral p30, or M-MuLV-specific RNA. Treatment with bromodeoxyuridine (50 microgram/ml for 24 h) resulted in induction of XC-positive NB-tropic virus, although only a small fraction of the cells released virus (less than 0.1% after 48 h). Immunofluorescent staining and flow microfluorometry indicated that a wave of p30 accumulation occurs in the induced cells, with a maximum at 24 to 48 h after the addition of bromodeoxyuridine. Furthermore, most, if not all, cells were induced to produce p30 protein. Similar kinetics were found for the accumulation of M-MuLV-specific RNA in the cytoplasm of induced cells. This rapid induction of virus expression in a majority of cells was dependent on the presence of the M-MuLV genome and probably represents primarily the expression of this endogenous virus since induction was not observed in cells similarly derived from a sibling embryo lacking the M-MuLV genome. 相似文献