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Md. Abdul Baten Takahiro Asano Keiichi Motohashi Yasushi Ishiguro Mohammad Ziaur Rahman Shigeki Inaba Haruhisa Suga Koji Kageyama 《Mycological Progress》2014,13(4):1145-1156
We examined the phylogenetic relationships among Phytopythium species using the rDNA ITS region, the LSU rDNA region, and the mitochondrial coxI and coxII genes. The genus was resolved into three monophyletic clades (1–3). Clade 1 was the largest clade, composed of 12 known species. Clades 2 contained two known and one new species candidate and clade 3 contained two known species. Three isolates in clade 2 (FP1, HonMa, and a strain designated as P. helicoides CBS293.35) formed a monophyletic group with high bootstrap support. This monophyletic group was distinct from P. helicoides sensu stricto. All three isolates came from damped-off buckwheat seedlings. The isolates were morphologically identical with one another and were characterized by globose, sub-globose, or pyriform sporangia with apical papillae; internally or internally nested proliferating sporangia; simple sympodia; coiling antheridial stalks; and wavy, sessile, or clavate antheridial cells. The isolates grew at temperatures between 15 °C and 40 °C, and the optimum temperature was 30 °C, with a radial growth rate of 20 mm/24 h. The phylogenetic and morphological analyses indicated that these isolates belong to a distinct species, which was previously under the genus Pythium, named here Phytopythium fagopyri comb. nov. 相似文献
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65.
Meherun Naher Keiichi Motohash Hideki Watanabe Yoshiaki Chikuo Masako Senda Haruhisa Suga Clive Brasier Koji Kageyama 《Mycological Progress》2011,10(1):21-31
A new species of Phytophthora was isolated from stem and root rot of chrysanthemum in the Gifu and Toyama prefectures of Japan. The species differs from
other Phytophthora species morphologically, and is characterized by nonpapillate, noncaducous sporangia with internal proliferation, formation
of both hyphal swellings and chlamydospores, homothallic nature, distinctive intercalary antheridia, and funnel-shaped oogonia.
The new species can grow even at 35°C, with an optimum growth temperature of 30°C in V8 juice agar medium. In phylogenetic
analyses based on five nuclear regions (LSU rDNA; genes for translation elongation factor 1α, β-tubulin, 60 S ribosomal protein
L10, and heat shock protein 90), the isolates formed a monophyletic clade. Although the rDNA ITS region shows a high resolution
and has proven particularly useful for the separation of Phytophthora species, it was difficult to align the sequences for phylogenetic analysis. Therefore, ITS region analysis using related
species as defined by the multigene phylogeny was performed, and the topology of the resulting tree also revealed a monophyletic
clade formed by the isolates of the species. The morphological characteristics and phylogenetic relationships indicate that
the isolates represent a new species, Phytophthora chrysanthemi sp. nov. In pathogenicity tests, chrysanthemum plants inoculated with the isolates developed lesions on stems and roots within
3 days, and the symptoms resembled the ones originally observed. Finally, the pathogen’s identity was confirmed by re-isolation
from lesions of infected plants. 相似文献
66.
Minoru Yoshida Haruhisa Ikumo Osamu Suzuki 《Bioscience, biotechnology, and biochemistry》2013,77(8):1208-1215
Bioassy procedure to evaluate biologically available energy of chemicals applicable to rats was established, and available energy of 36 chemicals was determined and compared with that estimated by chicks previously. Rats can utilize energy of propionic and butyric acids and n-hexyl propionate and butyrate well, while chicks cannot. Succinic acid, lauryl alcohol and dilauryl succinate at 5% dietary level were available by rats, though at 10% level lauryl alcohol was toxic. Ethyl lactate, octyl and decyl acetates and 1,2-propanediol dilaurate were available by both rats and chicks. Availability of other 6 esters including ethyl succinate and citrate was low. Availability and digestibility of aldehydes by rats were also low. 相似文献
67.
Haruhisa Kikuchi Yuzuru Kubohara Van Hai Nguyen Yasuhiro Katou Yoshiteru Oshima 《Bioorganic & medicinal chemistry》2013,21(15):4628-4633
Cellular slime molds are expected to have the huge potential for producing secondary metabolites including polyketides, and we have studied the diversity of secondary metabolites of cellular slime molds for their potential utilization as new biological resources for natural product chemistry. From the methanol extract of fruiting bodies of Polysphondylium filamentosum, we obtained new chlorinated benzofurans Pf-1 (4) and Pf-2 (5) which display multiple biological activities; these include stalk cell differentiation-inducing activity in the well-studied cellular slime mold, Dictyostelium discoideum, and inhibitory activities on cell proliferation in mammalian cells and gene expression in Drosophila melanogaster. 相似文献
68.
Tamama K Kawasaki H Kerpedjieva SS Guan J Ganju RK Sen CK 《Journal of cellular biochemistry》2011,112(3):804-817
Cell therapy with bone marrow multipotential stromal cells (MSCs) represents a promising approach to promote wound healing and tissue regeneration. MSCs expanded in vitro lose early progenitors with differentiation and therapeutic potentials under normoxic condition, whereas hypoxic condition promotes MSC self-renewal through preserving colony forming early progenitors and maintaining undifferentiated phenotypes. Hypoxia inducible factor (HIF) pathway is a crucial signaling pathway activated in hypoxic condition. We evaluated the roles of HIFs in MSC differentiation, colony formation, and paracrine activity under hypoxic condition. Hypoxic condition reversibly decreased osteogenic and adipogenic differentiation. Decrease of osteogenic differentiation depended on HIF pathway; whereas decrease of adipogenic differentiation depended on the activation of unfolded protein response (UPR), but not HIFs. Hypoxia-mediated increase of MSC colony formation was not HIF-dependent also. Hypoxic exposure increased secretion of VEGF, HGF, and basic FGF in a HIF-dependent manner. These findings suggest that HIF has a limited, but pivotal role in enhancing MSC self-renewal and growth factor secretions under hypoxic condition. 相似文献
69.
Ando K Uemura K Kuzuya A Maesako M Asada-Utsugi M Kubota M Aoyagi N Yoshioka K Okawa K Inoue H Kawamata J Shimohama S Arai T Takahashi R Kinoshita A 《The Journal of biological chemistry》2011,286(9):7619-7628
Synaptic loss, which strongly correlates with the decline of cognitive function, is one of the pathological hallmarks of Alzheimer disease. N-cadherin is a cell adhesion molecule essential for synaptic contact and is involved in the intracellular signaling pathway at the synapse. Here we report that the functional disruption of N-cadherin-mediated cell contact activated p38 MAPK in murine primary neurons, followed by neuronal death. We further observed that treatment with Aβ(42) decreased cellular N-cadherin expression through NMDA receptors accompanied by increased phosphorylation of both p38 MAPK and Tau in murine primary neurons. Moreover, expression levels of phosphorylated p38 MAPK were negatively correlated with that of N-cadherin in human brains. Proteomic analysis of human brains identified a novel interaction between N-cadherin and JNK-associated leucine zipper protein (JLP), a scaffolding protein involved in the p38 MAPK signaling pathway. We demonstrated that N-cadherin expression had an inhibitory effect on JLP-mediated p38 MAPK signal activation by decreasing the interaction between JLP and p38 MAPK in COS7 cells. Also, this study demonstrated a novel physical and functional association between N-cadherin and p38 MAPK and suggested neuroprotective roles of cadherin-based synaptic contact. The dissociation of N-cadherin-mediated synaptic contact by Aβ may underlie the pathological basis of neurodegeneration such as neuronal death, synaptic loss, and Tau phosphorylation in Alzheimer disease brain. 相似文献
70.
Iguchi H Urashima Y Inagaki Y Ikeda Y Okamura M Tanaka T Uchida A Yamamoto TT Kodama T Sakai J 《The Journal of biological chemistry》2007,282(26):19052-19061