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991.
Braz Tavares da Hora Júnior Davi Mesquita de Macedo Robert Weingart Barreto Harry C. Evans Carlos Raimundo Reis Mattos Luiz Antonio Maffia Eduardo S. G. Mizubuti 《PloS one》2014,9(8)
Background
South American leaf blight (SALB) of rubber has been the main constraint to production in its neotropical centre of origin since commercial plantations were first established. The fungal causal agent was identified and described more than a century ago but its precise placement within the Ascomycota still remains uncertain. Indeed, such is the ambiguity surrounding the pathogen that each of the spore morphs would, according to their present classification, be placed in different ascomycete families: the Microcyclus sexual morph in the Planistromellaceae and the two purported asexual morphs - Fusicladium and Aposphaeria – in the Venturiaceae and Lophiostomataceae, respectively. Given the historical importance of the fungus and the ever-menacing threat that it poses to rubber production in the Palaeotropics – and, thus to the rubber industry and to the global economy – its phylogeny, as well as its biology, should be resolved as a matter of urgency.Methods and Results
Here, six genomic regions (LSU rRNA, mtSSU, MCM7, EF-1α, Act and ITS) were used for reconstructing the molecular phylogeny of the SALB fungus based on material collected throughout Brazil. The analyses support the classification of the fungus in the family Mycosphaerellaceae s. str. (Capnodiales, Dothideomycetes) and place it firmly within the clade Pseudocercospora s. str., now accepted as one of the distinct genera within Mycosphaerellaceae. The new combination Pseudocercospora ulei is proposed and the life cycle of the fungus is confirmed, based on both experimental and phylogenetic evidence, with the Aposphaeria morph shown to have a spermatial rather than an infective-dispersal function.Conclusions
Because the phylogeny of the SALB fungus has now been clarified, new insights of its epidemiology and genomics can be gained following comparison with closely-related, better-researched crop pathogens. 相似文献992.
Andressa Jisely Barreto Barbosa Iracilda Sampaio Horacio Schneider Simoni Santos 《PloS one》2014,9(7)
The phylogenetic relationships within the Stellifer group of weakfishes (Stellifer, Odontoscion, Ophioscion, and Bairdiella) were evaluated using 2723 base pairs comprising sequences of nuclear (rhodopsin, TMO-4C4, RAG-1) and mitochondrial (16S rRNA and COI) markers obtained from specimens of nine species. Our results indicate a close relationship between Bairdiella and Odontoscion, and also that the genus Stellifer is not monophyletic, but rather that it consists of two distinct lineages, one clade containing S. microps/S. naso/S. brasiliensis and the other, S. rastrifer/S. stellifer/Stellifer sp. B, which is closer to Ophioscion than the former clade. The O. punctatissimus populations from the northern and southern Brazilian coast were also highly divergent in both nuclear (0.8% for rhodopsin and 0.9% for RAG-1) and mitochondrial sequences (2.2% for 16S rRNA and 7.3% for COI), which we conclude is consistent with the presence of two distinct species. The morphological similarities of the members of the Stellifer group is reinforced by the molecular data from both the present study and previous analyses, which have questioned the taxonomic status of the Stellifer group. If, on the one hand, the group is in fact composed of four genera (Stellifer, Ophioscion, Odontoscion, and Bairdiella), one of the two Stellifer clades should be reclassified as a new genus. However, if the close relationship and the reduced genetic divergence found within the group is confirmed in a more extensive study, including representatives of additional taxa, this, together with the morphological evidence, would support downgrading the whole group to a single genus. Obviously, these contradictory findings reinforce the need for a more systematic taxonomic revision of the Stellifer group as a whole. 相似文献
993.
Isabel Fernández-Pisonero Javier López Esther Onecha Ana I. Due?as Patricia Maeso Mariano Sánchez Crespo José Alberto San Román Carmen García-Rodríguez 《PloS one》2014,9(10)
Given that the bioactive lipid sphingosine 1-phosphate is involved in cardiovascular pathophysiology, and since lipid accumulation and inflammation are hallmarks of calcific aortic stenosis, the role of sphingosine 1-phosphate on the pro-inflammatory/pro-osteogenic pathways in human interstitial cells from aortic and pulmonary valves was investigated. Real-time PCR showed sphingosine 1-phosphate receptor expression in aortic valve interstitial cells. Exposure of cells to sphingosine 1-phosphate induced pro-inflammatory responses characterized by interleukin-6, interleukin-8, and cyclooxygenase-2 up-regulations, as observed by ELISA and Western blot. Strikingly, cell treatment with sphingosine 1-phosphate plus lipopolysaccharide resulted in the synergistic induction of cyclooxygenase-2, and intercellular adhesion molecule 1, as well as the secretion of prostaglandin E2, the soluble form of the intercellular adhesion molecule 1, and the pro-angiogenic factor vascular endothelial growth factor-A. Remarkably, the synergistic effect was significantly higher in aortic valve interstitial cells from stenotic than control valves, and was drastically lower in cells from pulmonary valves, which rarely undergo stenosis. siRNA and pharmacological analysis revealed the involvement of sphingosine 1-phosphate receptors 1/3 and Toll-like receptor-4, and downstream signaling through p38/MAPK, protein kinase C, and NF-κB. As regards pro-osteogenic pathways, sphingosine 1-phosphate induced calcium deposition and the expression of the calcification markers bone morphogenetic protein-2 and alkaline phosphatase, and enhanced the effect of lipopolysaccharide, an effect that was partially blocked by inhibition of sphingosine 1-phosphate receptors 3/2 signaling. In conclusion, the interplay between sphingosine 1-phosphate receptors and Toll-like receptor 4 signaling leads to a cooperative up-regulation of inflammatory, angiogenic, and osteogenic pathways in aortic valve interstitial cells that seems relevant to the pathogenesis of aortic stenosis and may allow the inception of new therapeutic approaches. 相似文献
994.
Leilane C. Barreto Queila S. Garcia Melanie Morales Maren Müller Sergi Munné-Bosch 《Plant Cell, Tissue and Organ Culture》2014,118(2):203-213
The fruit of the macaw palm (Acrocomia aculeata) may be used for biofuel production, but its exploitation as a crop is currently limited by its low germinability. Therefore, obtaining plantlets in vitro is an excellent way to solve this problem. Here we aimed to identify the optimal conditions for storing the fruit before obtaining plantlets and testing to what extent vitamin E and defense-related phytohormones are good indicators of embryo growth in vitro. We tested the effects of four storage conditions (nursery, laboratory, cold chamber and freezer) on seed germinability and embryo growth, and evaluated endogenous levels of vitamin E and defense-related phytohormones (abscisic acid, salicylic acid and jasmonic acid) in the endosperm and embryos. Low temperatures [both cold chamber (5 °C) and freezer (?18 °C) methods] killed the embryos, while storing the fruit in the laboratory was the most efficient method of obtaining plantlets, even after a year. Vitamin E and abscisic acid turned out to be good indicators of embryo growth. Enhanced vitamin E and abscisic acid levels had a strong positive correlation with successful embryo growth, thus indicating that these compounds are needed to protect the embryo during fruit storage. Furthermore, abscisic acid levels had a negative correlation with the percentage of contaminated embryos, thus suggesting that the endogenous physiological stage of the seeds affects subsequent contamination in in vitro cultures. We concluded that (1) storing fruit under laboratory conditions is the most efficient means of obtaining plantlets successfully, and (2) vitamin E and abscisic acid can be used as reliable indicators of embryo growth during in vitro culture. 相似文献
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996.
997.
Melissa Barreto Falcao Sergio Cimerman Kleber Giovanni Luz Alberto Chebabo Helena Andrade Brigido Iza Maria Lobo Artur Timerman Rodrigo Nogueira Angerami Clovis Arns da Cunha Helio Arthur Bacha Jesse Reis Alves Alexandre Naime Barbosa Ralcyon Francis Teixeira Leonardo Weissmann Priscila Rosalba Oliveira Marco Antonio Cyrillo Antonio Carlos Bandeira 《Annals of clinical microbiology and antimicrobials》2016,15(1):57
A panel of national experts was convened by the Brazilian Infectious Diseases Society in order to organize the national recommendations for the management of zika virus infection. The focus of this document is the diagnosis, both clinical and laboratorial, and appropriate treatment of the diverse manifestations of this infection, ranging from acute mild disease to Guillain-Barré syndrome and also microcephaly and congenital malformations. 相似文献
998.
Dual Role for the Tyrosine Decarboxylation Pathway in Enterococcus faecium E17: Response to an Acid Challenge and Generation of a Proton Motive Force 下载免费PDF全文
In this work we investigated the role of the tyrosine decarboxylation pathway in the response of Enterococcus faecium E17 cells to an acid challenge. It was found that 91% of the cells were able to remain viable in the presence of tyrosine when they were incubated for 3 h in a complex medium at pH 2.5. This effect was shown to be related to the tyrosine decarboxylation pathway. Therefore, the role of tyrosine decarboxylation in pH homeostasis was studied. The membrane potential and pH gradient, the parameters that compose the proton motive force (PMF), were measured at different pHs (pH 4.5 to 7). We obtained evidence showing that the tyrosine decarboxylation pathway generates a PMF composed of a pH gradient formed due to proton consumption in the decarboxylation reaction and by a membrane potential which results from electrogenic transport of tyrosine in exchange for the corresponding biogenic amine tyramine. The properties of the tyrosine transporter were also studied in this work by using whole cells and right-side-out vesicles. The results showed that the transporter catalyzes homologous tyrosine/tyrosine antiport, as well as electrogenic heterologous tyrosine-tyramine exchange. The tyrosine transporter had properties of a typical precursor-product exchanger operating in a proton motive decarboxylation pathway. Therefore, the tyrosine decarboxylation pathway contributes to an acid response mechanism in E. faecium E17. This decarboxylation pathway gives the strain a competitive advantage in nutrient-depleted conditions, as well as in harsh acidic environments, and a better chance of survival, which contributes to higher cell counts in food fermentation products. 相似文献
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