浅谈儿童癔病与护理实践

帮助患儿及家长正确认识疾病,正确判断躯体疾病和心理疾病;简要阐述对患癔病儿童的一些护理体会,使患儿了解癔病发作与本人情感体验有关,使之认识到某些性格特征与发病之间的关系,从而使患儿及家长减轻心理压力,树立战胜疾病的信心。     

中西医结合治疗脂肪肝64 例临床观察

目的:探讨中西医结合治疗脂肪肝的临床疗效。方法:将64例患者随机分为治疗组和对照组,其中治疗组43例患者在西药治疗的基础上加入HD肝病治疗仪经络穴位中药局部导入;对照组采用西药治疗。结果:两组临床疗效总有效率、血生化改变、B超、CT、影像学改变总有效率经统计学处理,P<0.05,有显著差异;治疗组优于对照组。结论:临床治疗观察表明,运用此法配合西药营养肝细胞改善代谢,发挥中西医的协同作用,疗效明显优于单纯运用西药。     

负性情绪的生物学基础

目前,心理学、生物学及社会学等多个学科都在关注负性情绪。本文从生物学的角度,介绍了负性情绪的大脑解剖结构,阐述了神经递质、神经肽、神经甾体与负性情绪的关系,并描述了负性情绪中激素、基因及环境三者之间的相互作用。     

腔内心电图储存功能双腔起搏器的临床随访

目的：探讨具有腔内心电图储存功能（stored EGMs）的双腔起搏器对房性快速心律失常诊断和疗效评价的临床应用。方法：选择20例植入stored EGMs功能起搏器患者,观察1个月房性快速心律失常的记录情况,根据储存的腔内心电图进行分析诊断和药物治疗,了解经过1个月药物治疗后腔内心电图和自动模式转换（AMS）发生次数的变化。结果：经药物治疗后,房性快速心律失常和自动模式转换（AMS）发生次数明显减少分别为111vs367,P〈0.05;82vs138,P〈0.05）。结论：通过对腔内心电图的分析,其结果可以作为临床用药和疗效判断的参考依据。     

改良包皮环扎术治疗小儿包皮过长和包茎

目的：探讨改良包皮环扎术治疗小儿外皮过长和包茎的效果。方法：对180例年龄3—14岁的包皮过长或包茎的患儿行改良包皮环扎术,观察疗效。结果：手术时间5—10分钟,出血量少于1ml,术后结扎环脱落时间8—20天,平均14天。无术后出血、包皮嵌顿等并发症。结论：改良包皮环扎术是治疗小儿包皮过长和包茎的较好术式,适宜作为门诊手术,也适合在基层医疗单位推广。     

血管内皮细胞功能紊乱与心血管疾病关系的研究进展

本文综述了血管内皮细胞合成与释放的一氧化氮、内皮素、血管紧张素Ⅱ等多种生物活性物质及VEC功能紊乱与心血管疾病的关系,为进一步深入研究提供了思路。     

植物抗寒性研究进展

低温是限制植物生长和分布的一种非生物胁迫因素。因此,提高植物的抗寒性对农业具有十分重要的意义。综述了植物抗寒性的最新进展,包括植物抗寒性机理研究,提高植物抗寒力的方法研究和应用情况;提出了在植物抗寒性研究中存在的问题,并对未来工作重点进行了展望。     

百合转基因的研究进展

综述了近年来国内外关于百合遗传转化系统的建立及其基因转化研究技术。着重讨论了百合基因转化受体系统建立的诱导培养基和抗生素浓度的筛选、遗传转化方法及基因调控表达等的研究动态。     

带npt-Ⅱ基因转基因水稻快速检测技术的建立

以转溶菌酶基因水稻纯系材料中花9号(ZH9(R))及其受体品种中花9号(ZH9(CK))为材料, 以ZH9(R)中携带的npt-Ⅱ基因作为辅助筛选标记, 利用抗生素对其进行处理, 建立了一套快速检测带npt-Ⅱ基因转基因水稻的技术体系。使用不同浓度的卡那霉素(Kanamycin, Kan)和G418溶液将ZH9(R)和ZH9(CK)成株离体叶片于室内室温下置于培养皿中浸泡处理, 通过观察叶片变化, 确定G418为检测带npt-Ⅱ基因转基因水稻的最佳抗生素, 将G418(溶液)80 mg/L浓度(处理4天)作为检测该类转基因水稻成株离体叶片的临界浓度。进一步用G418对ZH9(R)和ZH9(CK)种子、幼胚和幼苗进行了不同处理。确定: (1) G418(溶液)300 mg/L浓度(处理7天)作为检测该类转基因水稻种子的临界浓度; (2) G418(1/2 MS+0.5 mg/L 6-BA+1.5%蔗糖培养基)200 mg/L浓度(处理10天)作为检测该类转基因水稻幼胚的临界浓度; (3) G418(1/2 MS+0.5 mg/L 6-BA+1.5%蔗糖培养基)150 mg/L浓度(处理12天)作为检测该类转基因水稻幼苗的临界浓度, 并且通过PCR方法证实了上述结论。将这些结论应用于ZH9(R)转育后代叶片、种子、幼胚和幼苗群体的检测, 检测效果都非常明显。这为带npt-Ⅱ基因转基因水稻建立了一套简便、直观且准确的检测方法。     

Effect of silymarin on bladder overactivity in cyclophosphamide-induced cystitis rat model

The purpose of this study was to investigate the effects of silymarin, a phytotherapeutic agent, on bladder overactivity in a cyclophosphamide (CYP)-induced cystitis rat model. Female Wistar Albino rats received a single intraperitoneal injection of CYP (150 mg/kg) or saline and after 72 h, bladder function was evaluated by in vitro preparations of whole bladders and cystometry with continuous saline infusion under urethane anesthesia. Silymarin or a vehicle was orally given for 7 days in rats. CYP was injected on the 5th day of silymarin or vehicle treatment and then the animals were killed on the 8th day. CYP-treatment dramatically potentiated the basal spontaneous contractions of isolated whole bladders compared to control rats. In anesthetized rats, during continuous infusion cystometry, intercontraction interval (ICI) was significantly shorter, but bladder voiding pressure was not significantly changed in CYP-injected rats compared to control rats. In the CYP-injected group, silymarin treatment significantly decreased the amplitude, frequency (contractions/min) and area under the curve of spontaneous contractions, but failed to change carbachol-induced contraction in isolated whole bladder. Also, silymarin treatment significantly increased the ICI in comparison to the vehicle treatment. In the saline-injected group, no significant changes in the bladder function were observed between the silymarin and vehicle-treated groups. Histopathological examination showed that CYP-induced bladder inflammation tended to be lower in the silymarin+CYP-treated group. In conclusion, the oral administration of silymarin suppressed CYP-induced bladder overactivity. Silymarin may be considered as an attractive treatment for CYP-induced bladder overactivity.