An opiate binding site in the rat brain is highly selective for 4,5-epoxymorphinans

In vitro binding studies have demonstrated the existence of multiple opiate receptor types. An additional site in the rat brain (termed the lambda site) is distinct from the established types by its selectivity for 4,5-epoxymorphinans (such as naloxone and morphine). While the lambda site displays a high affinity for naloxone in vivo and in vitro in fresh brain membrane homogenates, these sites rapidly convert in vitro to a state of low affinity. The regional distribution of the lambda site in the brain is strikingly different from that of the classic opiate receptor types.     

Multi-time scale analysis of sugarcane within-field variability: improved crop diagnosis using satellite time series?

Within-field spatial variability is related to multiple factors that can be time-independent or time-dependent. In this study, our working hypothesis is that a multi-time scale analysis of the dynamics of spatial patterns can help establish a diagnosis of crop condition. To test this hypothesis, we analyzed the within-field variability of a sugarcane crop at seasonal and annual time scales, and tried to link this variability to environmental (climate, topography, and soil depth) and cropping (harvest date) factors. The analysis was based on a sugarcane field vegetation index (NDVI) time series of fifteen SPOT images acquired in the French West Indies (Guadeloupe) in 2002 and 2003, and on an original classification method that enabled us to focus on crop spatial variability independently of crop growth stages. We showed that at the seasonal scale, the within-field growth pattern depended on the phenological stage of the crop and on cropping operations. At the annual scale, NDVI maps revealed a stable pattern for the two consecutive years at peak vegetation, despite very different rainfall amounts, but with inverse NDVI values. This inversion is linked with the topography and consequently to the plant water status. We conclude that (1) it is necessary to know the crop growing cycle to correctly interpret the spatial pattern, (2) single-date images may be insufficient for the diagnosis of crop condition or for prediction, and (3) the pattern of vigour occurrence within fields can help diagnose growth anomalies.

Observation of X-ray lines from a gamma-ray burst (GRB991216): evidence of moving ejecta from the progenitor

We report on the discovery of two emission features observed in the x-ray spectrum of the afterglow of the gamma-ray burst (GRB) of 16 December 1999 by the Chandra X-ray Observatory. These features are identified with the Ly(alpha) line and the narrow recombination continuum by hydrogenic ions of iron at a redshift z = 1.00 +/- 0.02, providing an unambiguous measurement of the distance of a GRB. Line width and intensity imply that the progenitor of the GRB was a massive star system that ejected, before the GRB event, a quantity of iron approximately 0.01 of the mass of the sun at a velocity approximately 0.1 of the speed of light, probably by a supernova explosion.     

Involvement of mammalian Mus81 in genome integrity and tumor suppression

Mus81-Eme1 endonuclease has been implicated in the rescue of stalled replication forks and the resolution of meiotic recombination intermediates in yeast. We used gene targeting to study the physiological requirements of Mus81 in mammals. Mus81-/- mice are viable and fertile, which indicates that mammalian Mus81 is not essential for recombination processes associated with meiosis. Mus81-deficient mice and cells were hypersensitive to the DNA cross-linking agent mitomycin C but not to gamma-irradiation. Remarkably, both homozygous Mus81-/- and heterozygous Mus81+/- mice exhibited a similar susceptibility to spontaneous chromosomal damage and a profound and equivalent predisposition to lymphomas and other cancers. These studies demonstrate a critical role for the proper biallelic expression of the mammalian Mus81 in the maintenance of genomic integrity and tumor suppression.     

Properties of metallic nanowires: from conductance quantization to localization

Material structures of reduced dimensions exhibit electrical and mechanical properties different from those in the bulk. Measurements of room-temperature electronic transport in pulled metallic nanowires are presented, demonstrating that the conductance characteristics depend on the length, lateral dimensions, state and degree of disorder, and elongation mechanism of the wire. Conductance during the elongation of short wires (length l approximately 50 angstroms) exhibits periodic quantization steps with characteristic dips, correlating with the order-disorder states of layers of atoms in the wire predicted by molecular dynamics simulations. The resistance R of wires as long as l approximately 400 angstroms exhibits localization characteristics with In R(l) approximately l(2).     

A quasi-biennial oscillation signal in general circulation model simulations

The quasi-biennial oscillation (QBO) is a free atmospheric mode that affects the equatorial lower stratosphere. With a quasi-regular frequency, the mean equatorial zonal wind alternates from easterly to westerly regimes. This oscillation is zonally symmetric about the equator, has its largest amplitude in the latitudinal band from 20 degrees S to 20 degrees N, and has a mean period of about 27 months. The QBO appears to originate in the momentum deposition produced by the damping in the stratosphere of equatorial waves excited by diabatic thermal processes in the troposphere. The results of three 10-year simulations obtained with three general circulation models are reported, all of which show the development in the stratosphere of a QBO signal with a period and a spatial propagating structure that are in good agreement with observations without any ad hoc parameterization of equatorial wave forcing. Although the amplitude of the oscillation in the simulations is still less than the observed value, the result is promising for the development of global climate models.     

Progression of vertebrate limb development through SHH-mediated counteraction of GLI3

Distal limb development and specification of digit identities in tetrapods are under the control of a mesenchymal organizer called the polarizing region. Sonic Hedgehog (SHH) is the morphogenetic signal produced by the polarizing region in the posterior limb bud. Ectopic anterior SHH signaling induces digit duplications and has been suspected as a major cause underlying congenital malformations that result in digit polydactyly. Here, we report that the polydactyly of Gli3-deficient mice arises independently of SHH signaling. Disruption of one or both Gli3 alleles in mouse embryos lacking Shh progressively restores limb distal development and digit formation. Our genetic analysis indicates that SHH signaling counteracts GLI3-mediated repression of key regulator genes, cell survival, and distal progression of limb bud development.     

Gamma-Ray bursts: accumulating afterglow implications, progenitor clues, and prospects

Gamma-ray bursts (GRBs) are sudden, intense flashes of gamma rays that, for a few blinding seconds, light up in an otherwise fairly dark gamma-ray sky. They are detected at the rate of about once a day, and while they are on, they outshine every other gamma-ray source in the sky, including the sun. Major advances have been made in the last 3 or 4 years, including the discovery of slowly fading x-ray, optical, and radio afterglows of GRBs, the identification of host galaxies at cosmological distances, and evidence showing that many GRBs are associated with star-forming regions and possibly supernovae. Progress has been made in understanding how the GRB and afterglow radiation arises in terms of a relativistic fireball shock model. These advances have opened new vistas and questions on the nature of the central engine, the identity of their progenitors, the effects of the environment, and their possible gravitational wave, cosmic ray, and neutrino luminosity. The debates on these issues indicate that GRBs remain among the most mysterious puzzles in astrophysics.     

Chiral amplification of oligopeptides in two-dimensional crystalline self-assemblies on water

Differences in the two-dimensional packing arrangements of racemic and enantiomeric crystalline self-assemblies on the water surface of amphiphilic activated analogs of lysine and glutamic acid have been used to prepare oligopeptides of homochiral sequence and oligopeptides of single handedness from chiral nonracemic mixtures. The crystalline structures on the water surface were determined by grazing incidence x-ray diffraction and the diastereomeric composition of the oligopeptides by matrix-assisted laser desorption time-of-flight mass spectrometry with enantio-labeling. These results suggest that reactivity of ordered clusters at interfaces might have played a role in the generation of early homochiral biopolymers.