α_1受体对不同状态心肌细胞的作用

目的　通过激活和阻断不同状态心肌的α1 受体 ,探讨α1 受体的活化状态与不同状态心肌的关系。方法　采用Langedorff灌流技术复制动物模型。第 1个实验检验不同浓度的α1 受体激动剂 phenylephrine和阻断剂 prazosin(0 0 1、0 1、1、1 0、1 0 0 μmol·L- 1 )在缺血前 1 0min、缺血期和再灌注时的效应。第 2个实验研究 phenylephrine和 prazosin的时间依赖性。每组实验的末期 ,测量磷酸肌酸激酶、细胞凋亡和坏死情况。结果　 0 1、1 μmol·L- 1 的 phenylephrine可发挥最大效益 ,但prazosin浓度超过 1 0 μmol·L- 1 可产生有害作用。缺血前激活、缺血期间阻断α1 受体可保护心肌。再灌注时激活α1 受体效应不明确 ,但阻断α1 受体有害。结论　激动或阻断α1 受体所产生的效应与心肌的状态有关     

注射用阿莫西林钠克拉维酸钾细菌内毒素检查

目的建立注射用阿莫西林钠克拉维酸钾细菌内毒素检查方法.方法按中国药典2000年版附录细菌内毒素检查法要求进行试验.结果注射用阿莫西林钠克拉维酸的浓度为2.5mg/ml时不干扰鲎试剂与细菌内毒素的凝胶反应.结论细菌内毒素检查法适用于检测注射用阿莫西林钠克拉维酸钾中的内毒素.     

非小细胞肺癌微切割组织中3p杂合性丢失的研究

目的:探讨微切割非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中3p14.2和3p25等位基因的杂合性丢失(loss of heterozygosity,LOH)与NSCLC发生发展之间的相关性.方法:采用微切割技术和聚合酶链反应(PCR)结合二核苷酸(CA)n重复序列多态性方法,分析44例NSCLC石蜡切片中癌组织和非癌组织中3p14.2和3p25位点的杂合性丢失(LOH).结果:在44例NSCLC中29例组织标本检测3p14.2位点的LOH,其丢失率为65.5%(19/29),而另外15例患者中3p25的LOH的丢失率为40.0%(6/15).微切割的非癌组织均未见有3p的丢失,癌组织的3p LOH频率与肺癌组织学分类及TNM分期均无明显关系(P＞0.05).结论:NSCLC癌组织中普遍出现3p14.2及3p25杂合性丢失现象,从而提示该两处可能存在一个或多个与人体肺细胞癌变相关的抑癌基因.     

Effects of the glucoprotein component of musk on functions of rat polymorphonuclear leukocytes activated by LTB4 in vitro]

To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by LTB4, an in vitro incubation system with rat polymorphonuclear leukocytes was used. The superoxide anion production was determined by cytochrome C reduction, and the beta-glucuronidase and lysozyme release was quantitated by enzyme reactions in which phenolphthaleinglucuronic acid and micrococcus lysodeikticus were used as the substrates. In comparison with the control, musk-1 at final concentrations of 1 microgram/ml-100 micrograms/ml can increase the superoxide anion production by 28.7%-202.1% and decrease the beta-glucuronidase and lysozyme release by 3%-46% and 6%-32% respectively in rat polymorphonuclear leukocytes. It is concluded that musk-1 can significantly affect the functions of rat polymorphonuclear leukocytes activated by LTB4. One of the mechanisms of this anti-inflammatory action of musk may consist in the inhibition of lysosomal enzyme release.     

LOSS OF HETEROZYGOSITY ON CHROMOSOME 13 IN SQUAMOUS CELL CARCINOMAS OF THE LARYNX

ＬＯＳＳＯＦＨＥＴＥＲＯＺＹＧＯＳＩＴＹＯＮＣＨＲＯＭＯＳＯＭＥ１３ＩＮＳＱＵＡＭＯＵＳＣＥＬＬＣＡＲＣＩＮＯＭＡＳＯＦＴＨＥＬＡＲＹＮＸＢａｉＳｕｊｕａｎ白素娟ＺｈａｎｇＸｕｅ张学ＷａｎｇＪｕｎ王筠ＳｕｎＫａｉｌａｉ孙开来ＦｅｉＳｈｅｎｇｚｈｏｎ...     

蒙药广枣中3种黄酮类成分对离体心功能的影响

采用Langendorff灌流法,观察广枣中3种黄酮类成分对离体心功能的影响,为找到一种抗心律失常作用较强的主要化学单体或简单复合物打下基础.结果表明：样品1和样品2减慢心率作用在0.027g生药1/ml～0.22g生药/ml范围内,具有明显的浓度依赖性,当浓度大于0.22g生药/ml时,减慢心率作用反而随着剂量增加而减小;样品3减慢心率作用与剂量成反比.在0.027g生药1ml～1.72g生药/ml范围内,样品1、样品2和样品3使心肌收缩幅度的变化均无统计学意义.结论：广枣中3种黄酮类成分均可减慢心率,对心肌收缩力影响不明显.     

MRP-1/CD9和HSP60在膀胱癌中的表达

目的:探讨MRP-1/CD9和HSP60的表达与膀胱癌发生发展的关系.方法:应用免疫组织化学技术(SP法)检测CD9和HSP60在75例膀胱移行细胞癌标本,15例正常膀胱组织中的表达.结果:CD9和HSP60在正常膀胱组织均高表达,41.2%和42.7%的膀胱癌对CD9和HSP60显示了表达减低.不同临床分期和病理分级的膀胱癌中CD9的表达有差异(P＜0.05),根据预后表浅性膀胱癌的复发浸润组和非浸润组中CD9和HSP60表达有差异(P＜0.05).结论:CD9和HSP60可能与膀胱癌的发生发展有关,它们可能作为判断膀胱癌预后的新指标.     

复合诱变筛选avermectins高产菌株

由Streptomyces avermitilis初始菌株经过自然分离可以得到三种不同类型的菌株，即灰色粉末型、白色和光秃型。其中灰色粉末型产素水平最高，摇瓶发酵效价达到6835．1μg／ml，B1组分达到56．3％。经过紫外线和亚硝基胍对初始菌株的孢子的诱变及对其原生质体的诱变再生。并且经过异亮氨酸和链霉素的定向筛选，得到的高产菌株摇瓶发酵效价达到8542．9μg／ml，B。组分达到64．9％；同时通过对高产菌株进行传代培养检验其传代稳定性，结果表明，传代3代后，菌株开始退化，并且其发酵效价有比较显著的下降。     

900MHz微波电磁辐射对原代培养的大鼠脑皮质神经元能量代谢的影响

目的　研究 90 0MHz微波电磁辐射对原代培养的大鼠脑皮质神经元能量代谢的影响。方法　将大鼠脑皮质神经元暴露于 90 0MHz的连续性微波电磁辐射 (SAR =3 2 2mW g、PD =9mW cm2 ) ,每天暴露 2h ,连续 4d或 5d ,及一次性 1 2h暴露 ,以细胞色素氧化酶为观察指标 ,研究微波对神经元能量代谢的影响。结果　微波电磁辐射可使神经元细胞色素氧化酶活性降低。结论　神经元细胞色素氧化酶活性的改变并非“致热效应”所致 ;微波电磁辐射对神经元细胞色素氧化酶活性影响有蓄积毒性作用 ,其影响在一定程度上是可恢复的 ,并且与神经元接受微波辐射时细胞培养年龄关系不密切。     

Interaction and Relationship Between Angiotensin Converting Enzyme Gene and Environmental Factors Predisposing to Essential Hypertension in Mongolian Population of China

Objective To investigate the association of specific functional gene ACE (I/D) variants of the renin-angiotensin system with essential hypertension (EH) and interaction between ACE (I/D) gene and risk factors for EH in a genetically homogenous Mongolia rural population of China. Methods Individuals (n=1099) were recruited from general population of Kezuohouqi Banner in Inner Mongolian Autonomous Region. Results The association was found between ACE genotype DD plus ID and EH, with an interaction between ACE genotype DD plus ID and cigarette smoking in an additive model. Cigarette smoking index and ACE gene showed a low exposure-gene (LEG) effect on EH, with interaction indices from 7.10 to 1.16. Interaction between ACE genotype DD plus ID and alcohol drinking on EH appeared an additive model. Alcohol drinking index and ACE gene showed a low exposure-gene (LEG) effect on EH, with interaction indices from 1.66 to 1.09. BMI and ACE gene showed a low exposure-gene (LEG) effect on EH, with interaction indices from 6.15 to 2.49.Interactions between ACE genotype and WHR on EH showed a multiplicative model. In a short, there was an interaction between ACE gene and cigarette smoking, alcohol drinking and BMI on EH,especially in a low dose-exposure effect.Cpmchusion It is important for individuals who carry ACE D allele gene to prevent FH, and furthermore, to prevent and control coronary heart disease, in a view of population-based prevention.