Increased frequency of CD4+ CD25+ FOXP3+ cells correlates with the progression of 4-nitroquinoline1-oxide-induced rat tongue carcinogenesis

Objectives

CD4+ CD25+ FoxP3+ T cells (Tregs) play an essential role in sustaining self-tolerance by negatively regulating immune responses. Increased frequencies of Tregs have been reported in a variety of human cancers. The aim of this study was to evaluate the prevalence of Tregs infiltration in the peripheral blood and regional lymph nodes during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide (4NQO).

Materials and methods

Forty-eight Sprague–Dawley rats were divided into the control (n?=?16) and experimental groups (n?=?32) to which 4NQO in drinking water was administered. Flow cytometry was used to analyze the prevalence of Tregs in lymphocytes of peripheral blood and regional lymph nodes during 4NQO-induced rat tongue carcinogenesis. CD4+ CD25+ FoxP3+ cells were expressed as a percentage of the total CD4+ cells.

Results

The frequency of Tregs in peripheral blood from squamous cell carcinoma rats was significantly higher than controls (3.82?±?0.62 versus 1.40?±?0.31 %, P?P?Conclusion and clinical relevance Tregs in peripheral blood and lymph nodes were associated with disease progression during 4NQO-induced rat tongue carcinogenesis. This study indicated that the upregulation of Tregs might play important role during oral mucosa malignant transformation.     

乳腺癌术后康复期患者对医护联合康复门诊需求的研究

目的深入了解乳腺癌术后康复期患者对医护联合康复门诊的需求状况,完善医护联合康复门诊的服务内容。 方法采用质性研究的现象学方法,选取13例乳腺癌术后患者进行半结构式深入访谈,并运用Colaizzi现象学7步分析法对资料进行分析。 结果提炼出3个主题和5个亚主题：延续性的患肢康复需求（患肢功能障碍的康复需求、淋巴水肿管理的需求）;多样化的信息需求（对健康生活方式的关注、对性健康及生育相关知识的需求、对辅助治疗相关知识的需求）;专业和持续的情感支持需求。 结论乳腺癌术后康复期患者对医护联合康复门诊存在较多方面的需求,应根据患者需求设置并细化门诊服务内容,为患者提供延续护理服务,改善乳腺癌术后患者的长期生活质量。     

口腔颌面部锥形束CT结合微创拔牙技术在下颌阻生第三磨牙拔除中的临床应用

目的研究口腔颌面部锥形束CT(CBCT)检查结合牙体分割微创拔牙技术在下颌阻生第三磨牙(IMTM)拔除中的临床应用。方法选取2018年1月至2019年5月四川省攀枝花市中心医院诊断为IMTM的97例(155颗)拔牙患者为研究对象。术前CBCT明确IMTM与周围重要组织的关系,术中采用转角高速涡轮机夹持长臂裂钻分割牙体,以微创的方式分块拔除IMTM。记录拔牙时间、患者满意度、术中及术后并发症等指标。采用t检验比较男、女间拔牙时间的差异,采用方差分析比较不同阻生牙类型及不同年龄段组间拔牙时间的差异。结果CBCT结果显示67.7%(105/155)的下颌管位于IMTM根尖的颊侧、22.6%根尖下方、9.7%舌侧,其中24.5%<1 mm;24颗(15.5%,24/155)IMTM根尖区舌侧骨板缺如,56颗牙(36.1%,56/155)<1 mm。IMTM以近中、垂直及水平向阻生最为常见,平均拔牙时间分别是(24.3±1.1)min,(15.6±1.2)min,(29.7±1.8)min,差异有统计学意义(F=23.85,P<0.001);术中8颗牙(5.2%,8/155)下颌管暴露,无1例舌侧骨板损伤致牙根移位。术后3例少量出血,1例发生干槽症,无1例发生下唇麻木,患者满意度在95.5%以上。结论CBCT应作为IMTM拔除前常规的影像学检查手段。高速涡轮机牙体分割微创拔牙技术适用于各类IMTM拔除,具有创伤小、临床效率高、并发症少、患者满意度高等优点。     

Decellularized nerve matrix hydrogel and glial‐derived neurotrophic factor modifications assisted nerve repair with decellularized nerve matrix scaffolds

Nerve defects are challenging to address clinically without satisfactory treatments. As a reliable alternative to autografts, decellularized nerve matrix scaffolds (DNM‐S) have been widely used in clinics for surgical nerve repair. However, DNM‐S remain inferior to autografts in their ability to support nerve regeneration for long nerve defects. In this study, we systematically and clearly presented the nano‐architecture of nerve‐specific structures, including the endoneurium, basement membrane and perineurium/epineurium in DNM‐S. Furthermore, we modified the DNM‐S by supplementing decellularized nerve matrix hydrogel (DNMG) and glial‐derived neurotrophic factor (GDNF) and then bridged a 50‐mm sciatic nerve defect in a beagle model. Fifteen beagles were randomly divided into three groups (five per group): an autograft group, DNM‐S group and GDNF‐DNMG‐modified DNM‐S (DNM‐S/GDNF@DNMG) group. DNM‐S/GDNF@DNMG, as optimized nerve grafts, were used to bridge nerve defects in the same manner as in the DNM‐S group. The repair outcome was evaluated by behavioural observations, electrophysiological assessments, regenerated nerve tissue histology and reinnervated target muscle examinations. Compared with the DNM‐S group, limb function, electrophysiological responses and histological findings were improved in the DNM‐S/GDNF@DNMG group 6 months after grafting, reflecting a narrower gap between the effects of DNM‐S and autografts. In conclusion, modification of DNM‐S with DNMG and GDNF enhanced nerve regeneration and functional recovery, indicating that noncellular modification of DNM‐S is a promising method for treating long nerve defects.     

连翘酯苷A对小鼠压力负荷性心力衰竭的保护作用及潜在机制

目的 探究连翘酯苷A(FTA)对小鼠压力负荷性心力衰竭(HF)的保护作用及其潜在的机制.方法 30只健康雄性C57BL/6小鼠随机分成Sham组、HF组和HF+FTA组.采用主动脉弓缩窄建立小鼠压力负荷性HF.称取小鼠体重及心脏重量并计算心脏重量指数;HE染色观察组织病理学改变;ELISA检测小鼠血清内炎症因子改变;W...     

Effects of Intercooling Intensity on Temperature Field and Microstructure of Large-Scale 2219 Al Alloy Billet Prepared by Internal Electromagnetic Stirring Casting

Internal electromagnetic stirring is an advanced melt treatment method, which can be used in direct chill casting to prepare large-scale Al alloy billets. Intercooling intensity is a primary parameter of internal electromagnetic stirring; its effects on temperature fields and microstructures have been investigated via numerical simulations and industrial experiments, respectively. The simulated results show an increase in the intercooling affected area and a decrease in sump depth with an increase in the intercooling heat transfer coefficient. The heat transfer coefficient should not exceed 500 W/(m² °C) because the solid fraction of the intercooling end bottom may exceed 50%. The experiment’s results demonstrate that the average grain sizes in the edge, 1/2 radius, and center are 151 ± 13 μm, 159 ± 14 μm, and 149 ± 16 μm, respectively, under a liquid nitrogen flow rate of 160 L/min, which is much finer than that of 80 L/min and more homogeneous than that of 240 L/min. Furthermore, an experimental liquid nitrogen flow rate of 80 L/min, 160 L/min, and 240 L/min approximately correspond to the simulated heat transfer coefficient of 200 W/(m² °C), 300 W/(m² °C), and 400 W/(m² °C), respectively.     

选择性环氧合酶2抑制剂对人膀胱癌细胞T24增殖和凋亡的作用

背景与目的:环氧合酶2(cyclooxygenase-2,COX-2)与膀胱癌的发生发展密切相关,因此COX-2抑制剂对于膀胱癌可能具有潜在的抗肿瘤效应.本实验旨在探讨选择性COX-2抑制剂对人膀胱癌细胞T24增殖和凋亡的抑制作用.方法:用四甲基偶氮唑蓝还原法(MTT)研究选择性COX-2抑制剂SC-58125、塞来昔布(celecoxib)和非选择性COX抑制剂吲哚美辛(indomethacin)对T24细胞增殖的影响;用流式细胞术、DNA电泳和Hoechst33258荧光染色3种方法检测SC-58125作用下T24细胞的凋亡,同时逆转录聚合酶链反应(RT-PCR)检测细胞凋亡相关基因Bcl-2和Bax基因的变化.结果:在12.5 μmol/L至200 μmol/L的剂量范围内,SC-58125、塞来昔布及吲哚美辛均能不同程度地抑制T24细胞的增殖,以SC-58125的抑制作用最强,IC50在25 μmol/L～50 μmol/L之间;3种凋亡检测方法均观察到SC-58125作用后凋亡细胞的比例增加,其中100 μmol/L的SC-58125作用于T24细胞6 h和12 h后,流式细胞仪测得凋亡细胞的比例分别为(7.95±1.88)%和(12.5±2.42)%,较对照组增加(P＜0.05),但细胞内与凋亡相关的Bcl-2和Bax基因表达没有变化.结论:选择性COX-2抑制剂体外可抑制T24细胞的增殖,并诱导细胞凋亡.