恶性疟P190抗原保守区Ⅰ、Ⅴ基因的亚克隆及在大肠杆菌中高效表达

作者采用PCR方法克隆了我国海南省FCC1/HN株P190抗原两个保守区基因,分别定名为P190CRI和P190CRV。基因片段经纯化后连接到pUC18载体中进行DNA序列分析,结果显示:除了P190CRV中有5个碱基变换外,其余序列均与MAD20型序列一致。经序列分析的两个基因片段分别与pGEX-2T载体连接,经双酶切鉴定后转化感受态JM109(DE_3)大肠杆菌进行高效融合表达,并且用Sepharose 4B-谷胱甘肽层析柱进行亲和纯化,结果为:两个插入基因片段均得到高效融合表达,经一步亲和纯化后就取得高纯度的重组蛋白。     

方剂学科发展思路与方法

方剂学科传统模式已发生重大变化，现代方剂学以计算机和实验为重要研究手段，熔理论方剂学、实验方剂学及临床方剂学于一炉。现代方剂学科模式的建立必将培育出新的知识增长点，丰富学科内涵，促进研究方向和专业课程的调整，提高学术水平和人才培养质量，实现新世纪方剂学科发展质的飞跃。     

海南省消灭脊髓灰质炎开展强化免疫活动的效果评价

笔者根据海南省脊髓灰质炎历年的发病情况，以常规免疫和省内及全国强化免疫资料，对该省消灭脊髓效果进行了评价。结果表明：１９９３、１９９４年两年全国强化免疫活动日的效果显著，海南省从１９９４年以来无脊灰野毒感染病例发生。实验室资料也证实，全国强化免疫活动日，起到了阻断Ⅰ型野毒株循环的作用。     

ENDOURETHRAL URETHROPLASTY WITH PREPUCE GRAFT FOR LONG POSTERIOR URETHRAL STRICTURE

Twenty patients underwent endourethral urethroplasty with free prepuce graft in combination with transurethral resection of scar for posterior urethral stricture. Two-year follow-up showed that they had good continent and free voiding results. Problems about surgical technique were detailed. We conclude that a free full-thickness prepuce evenly patched by means of the tube balloon to the freshly prepared wound derived from the stricture is the key to surgical success.     

三袖套管法大鼠肺移植

我们将血管套接技术用于大鼠肺移植中。在肉眼下，完成肺动静脉及支气管的吻合。在连续６例手术中，手术平均时间６１±４分钟，移植物总缺血时间１７±３分钟，手术时间缩短至国外同类手术的１／４，缺血时间缩短至同类手术的１／３。同时手术成功率达到７５％（６／８），手术后生存率１００％。行对侧肺切除术后，血气分析正常，显示了移植肺良好的气体交换功能     

酸性染料比色法测定甲氧异腈冻干品含量

本文采用酸性洒料比色法测定甲氧异腈冻干品的含量。结果表明，使用溴甲酚紫１．５ｍｌ，ＰＨ值为６．０的磷酸盐缓冲液５ｍｌ，氯仿５ｍｌ提取，经振摇２ｍｉｎ，静置１５ｍｉｎ为较理想比色条件，显色液稳定，甲氧异腈浓度在４－２０μｇ／ｍｌ范围内与吸收度呈良好线性关系。     

肝癌化疗栓塞术后综合征临床分析

本文分析报告192例各型肝癌化疗栓塞术后综合征发生情况:总发生率为89.1%,转氨酶升高发生率为77.2%,肝区疼痛64.1%,发热54.2%,恶心呕吐35.4%,腹胀38.0%,腹水形成19.8%,黄疸18. 8%,呃逆5.7%.结合我们的研究和经验讨论了肝癌化疗栓塞的病理生理改变,化疗栓塞术后综合征发生机理及临床对症处理方法.     

全反式维甲酸治疗急性早幼粒细胞白血病20例临床疗效观察

单用全反式维甲酸(RA)诱导治疗急性早幼粒细胞白血病(APL)20例,完全缓解率达90%,诱导天数短(中位数为32d)。治疗过程中未发生DIC,亦无因强烈化疗引起骨髓抑制后造成的感染和出血的危险。该药的副反应轻,患者均能耐受。该组的中位生存期已达630d,可望长期存活。     

Fast reconstruction with uniform noise properties in halfscan computed tomography

The hybrid algorithms developed recently for the reconstruction of fan-beam images possess computational and noise properties superior to those of the fan-beam filtered backprojection (FFBP) algorithm. However, the hybrid algorithms cannot be applied directly to a halfscan fan-beam sinogram because they require knowledge of a fullscan fan-beam sinogram. In this work, we developed halfscan-hybrid algorithms for image reconstruction in halfscan computed tomography (CT). Numerical evaluation indicates that the proposed halfscan-hybrid algorithms are computationally more efficient than are the widely used halfscan-FFBP algorithms. Also, the results of quantitative studies demonstrated clearly that the noise levels in images reconstructed by use of the halfscan-hybrid algorithm are generally lower and spatially more uniform than are those in images reconstructed by use of the halfscan-FFBP algorithm. Such reduced and uniform image noise levels may be translated into improvement of the accuracy and precision of lesion detection and parameter estimation in noisy CT images without increasing the radiation dose to the patient. Therefore, the halfscan-hybrid algorithms may have significant implication for image reconstruction in conventional and helical CT.