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11.
The expression of B7-H1 on keratinocytes in chronic inflammatory mucocutaneous disease and its regulatory role 总被引:8,自引:0,他引:8
Youngnak-Piboonratanakit P Tsushima F Otsuki N Igarashi H Machida U Iwai H Takahashi Y Omura K Yokozeki H Azuma M 《Immunology letters》2004,94(3):215-222
PD-1 and its ligands, B7-H1/PD-L1 and B7-DC/PD-L2, have been identified recently as CD28-B7 family molecules that are implicated in immune regulation. Lichen planus (LP) is a T cell-mediated chronic inflammatory mucocutaneous disease. We investigated the expression and function of PD-1 and its two ligands in LP. Immunohistochemical examination revealed the abundant expression of PD-1 and B7-H1 in infiltrating T cells and macrophages, and lower-level expression of B7-DC on macrophages in the subepithelium. Interestingly, substantial expression of B7-H1 on keratinocytes (KCs) was found close to the numerous T cell infiltrates in the subepithelium. Unstimulated cultured KCs expressed both B7-H1 and B7-DC, and their expression was upregulated by proinflammatory cytokines, particularly IFN-gamma. The T-cell proliferative responses and IFN-gamma production that were induced by IFN-gamma-treated KCs were augmented preferentially by anti-B7-H1 mAb, but not by anti-B7-DC mAb. These results indicate the regulatory role of B7-H1 on KCs in the interactions with T cells. Our results suggest that the induction of B7-H1 on KCs may play an important role in tolerance induction in the inflamed oral mucosa and skin. 相似文献
12.
Nakagawa Ichiro Park HunSoo Kotsugi Masashi Motoyama Yasushi Myochin Kaoru Takeshima Yasuhiro Matsuda Ryosuke Nishimura Fumihiko Yamada Syuichi Takatani Tsunenori Kichikawa Kimihiko Nakase Hiroyuki 《Neurosurgical review》2021,44(3):1493-1501
Neurosurgical Review - The present study aimed to determine the incidence of intraprocedural motor-evoked potential (MEP) changes and to correlate them with intraprocedural ischemic complications... 相似文献
13.
Okihiro Nishi Tsutomu Hara Takako Hara Yuji Sakka Fumihiko Hayashi Katsuhiko Nakamae Yoshibaru Yamada 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》1992,230(1):47-55
In this report we describe the surgical details involved in refilling the lenses of 13 rabbit and 3 primate eyes using an inflatable endocapsular balloon to restore accommodation. The procedure involves endocapsular phacoemulsification through a small buttonhole or dumbbell anterior capsulotomy or minicircular capsulotomy and the simultaneous preservation of capsular integrity, including the zonules and ciliary muscles. An inflatable balloon made of thin silicone membrane is then inserted into the empty capsular bag. A liquid silicone polymer is injected into the balloon through a delivery tube, and the empty capsular bag is refilled by the inflated balloon. The procedure was found to be reproducible, and an accommodation of 6 D was confirmed in one primate eye. Capsular opacification occurred, but the proliferation and migration of residual lens epithelial cells could be hindered by abundant refilling. This lens-refilling technique may provide restoration of accommodation in future cataract surgery.
Offprint requests to: O. Nishi 相似文献
14.
Fumihiko Kawamoto Nobuo Kido Takamasa Hanaichi Mustafa B. A. Djamgoz Robert E. Sinden 《Parasitology research》1992,78(4):277-284
Ionic regulation in the induction of exflagellation ofPlasmodium berghei was investigated by culturing the parasites in various isotonic media. Of the salts tested, NaHCO3 exhibited the highest activity in inducing exflagellation, whereas KHCO3 showed no activity. In the absence of HCO
3
–
, media containing monovalent cation (Na+, K+, Cs+, Rd+, choline+, lysine+, arginine+) and Cl– also induced exflagellation, but their activities were lower than that of NaHCO3. Anions of Br– or NO
3
–
could be substituted with Cl–, whereas other anions such as I–, NO
2
–
, SO
4
2–
, SCN–, H2PO
4
–
, or HPO
4
2–
failed to induce exflagellation, as did tetramethylammonium-Cl, CaCl2, MgSO4, MgCl2 and sucrose as well. These results suggest that the induction of exflagellation requires the presence of Na+ and HCO
3
–
or monovalent, membrane-permeable cation and Cl– in the medium. Measurements of the efflux of H[14C]O
3
–
or Cl– indicated that these anions were released from the cells into the NaCl or the NaHCO3 medium, respectively, probably by exchange in HCO
3
–
/Cl–. Determination of intracellular ionic concentrations by electron microscopic X-ray microanalysis of cryopreserved specimens revealed that in the NaHCO3 medium, external Na+ (and probably HCO
3
–
) enters the gametocytes by exchange with internal Cl– (and probably H+), whereas in Cl–-containing media, external unspecified cation and Cl– influx by exchange, probably with H+ and HCO
3
–
. It is therefore suggested that two separate ion exchangers, i.e., Na+-dependent HCO
3
–
(in)/Cl–(out) and nonspecific monovalent-cation-dependent Cl–(in)/HCO
3
–
(out) exchangers, are involved in the induction of gametogenesis inP. berghei. Furthermore, the presence of both classes of anion in the medium enhanced exflagellation activity and increased Na+ uptake more than did the NaCl or NaHCO3 medium alone. The apparent synergistic enhancement by two contraactive anion exchangers is consistent with a recycling model of pHi regulation, in which HCO
3
–
and Cl– are exchanged between the cells and the media, resulting in the acceleration of monovalent cation/H+ exchange.This work was supported by a Grant-in-Aid (No. 01570212) from the Ministry of Education, Science and Culture, Japan and the Ohyama Health Foundation, Japan (to FK), and in part by the Medical Research Council, United Kingdom (to RES) 相似文献
15.
Fumihiko Niiya Shinya Nishizaka Kazuko Matsunaga Kikuo Koufuji Masaki Mori Hitoshi Katai Hideaki Yamana Kyogo Itoh 《Cancer science》2000,91(3):337-342
We previously reported SART3 as a tumor-rejection antigen recognized by histocompatibility leukocyte antigen (HLA)-A24-restricted cytotoxic T lymphocytes (CTLs). In this study, we investigated the expression of the SART3 antigen in gastric cancers, as a candidate for use in specific immunotherapy. The SART3 antigen was detected in 9 of 10 (90%) gastric cancer cell lines, 35 of 52 (67.3%) gastric cancer tissues, and 0 of 20 non-tumorous gastric tissues. SART3-derived peptides corresponding to positions 109–118 and 315–323 induced HLA-A24-restricted and tumorspecific CTLs from peripheral blood mononuclear cells (PBMCs) of gastric cancer patients. These peptide-induced CTLs recognized HLA-A24+ SART3+ gastric cancer cells, but not HLA-A24+ SART3− or HLA-A24− SART3+ gastric cancer cells. Therefore, the SART3 peptides could be useful in specific immunotherapy of gastric cancer patients. 相似文献
16.
Kuniko Sato Fumihiko Suzuki Hiromasa Tsujiguchi Akinori Hara Takayuki Kannon Sakae Miyagi Keita Suzuki Masaharu Nakamura Chie Takazawa Aki Shibata Hirohito Tsuboi Yukari Shimizu Thao Thi Thu Nguyen Tadashi Konoshita Yasuki Ono Koichi Hayashi Atsushi Tajima Hiroyuki Nakamura 《Nutrients》2022,14(23)
Epidemiological studies reported that resilience, generally regarded as the ability to manage stress in the face of adversity, correlates with mental health in middle-aged and older adults. Currently, there is limited information on eating habits that affect resilience. Therefore, this cross-sectional study investigated the relationship between vitamin intake and resilience based on sex in community-dwelling middle-aged and older individuals in Shika town, Ishikawa Prefecture, Japan. A total of 221 participants (106 men and 115 women) aged 40 years or older were included in the analysis. We assessed vitamin intake and resilience using a brief-type self-administered diet history questionnaire (BDHQ) and the resilience scale (RS), respectively. A two-way analysis of covariance (ANCOVA) revealed that higher intakes of β-carotene and vitamin K were associated with higher RS in women, but not in men. Furthermore, a multiple logistic regression analysis stratified by sex showed that β-carotene and vitamin K were significant independent variables for RS only in women. The present study suggests that higher intakes of β-carotene and vitamin K were associated with higher resilience among middle-aged and older women. The results obtained demonstrate that β-carotene and vitamin K intakes may enhance resilience by strengthening stress tolerance. 相似文献
17.
In vitro antifungal activities of naphtoquinone-derivatives, which are constituents of Shikon, roots of Lithospermum erythrorhizon, were investigated against several fungal pathogens. When the biological activity of these compounds was tested against fungi, a wide range of sensitivity was recorded. Shikonin was found to have a stronger than fluconazole against yeast-like fungi: four-fold against Candida krusei (minimal inhibitory concentration (MIC); 4 microg/ml) and two-fold (MIC; 4 microg/ml) against Saccharomyces cerevisiae, though it showed the same potency as fluconazole against C. glabrata. Deoxyshikonin also exhibited four-fold stronger activity against C. krusei (MIC; 4 microg/mi) and three-fold (MIC; 2 microg/ml) stronger against S. cerevisiae. Acetylshikonin and beta-hydroxyisovaleryl shikonin showed lower activities against all fungal pathogens except for C. krusei compared with the standard. Against the filamentous fungus, Trichosporon cutaneum, all naphthoquinones were found to have a range of activity with lower potency than standard. This result provides a rational basis for the clinical use of shikon and shows the possibility of its use in medicinal treatment as an anti-inflammatory agent with antifungal activity. 相似文献
18.
Haruka Ejiri Mahiro Asano Nana Nakahata Satoshi Suzuki Ayako Sato Natsuki Nagamine Chisato Takahashi Yukie Yamaya Manabu Iwadate Takashi Matsuzuka Tetsuya Ohira Seiji Yasumura Satoru Suzuki Fumihiko Furuya Hiroki Shimura Shinichi Suzuki Susumu Yokoya Hitoshi Ohto Kenji Kamiya 《Clinical Pediatric Endocrinology》2023,32(1):52
19.
Keita Suzuki Hiromasa Tsujiguchi Akinori Hara Sakae Miyagi Thao Thi Thu Nguyen Yasuhiro Kambayashi Yukari Shimizu Fumihiko Suzuki Chie Takazawa Masaharu Nakamura Hirohito Tsuboi Takayuki Kannon Atsushi Tajima Hiroyuki Nakamura 《Nutrients》2022,14(24)
The relationship between calcium intake and bone strength in older Asian individuals, including Japanese, is controversial; therefore, we herein investigated this relationship in older Japanese populations. We performed a cross-sectional analysis of 314 participants older than 65 years who voluntarily participated in a medical examination and responded to questionnaires. The osteo-sono assessment index (OSI) measured at the right calcaneus using a quantitative ultrasonic device was used as an indicator of bone strength. The daily dietary intake of calcium was assessed using a brief-type self-administered diet history questionnaire. A two-way analysis of covariance revealed a significant interaction between sex and calcium intake on the OSI (p < 0.01). A multiple regression analysis showed a positive correlation between calcium intake and the OSI in males (p < 0.01), but not females (p = 0.27). In females, grip strength divided by body weight positively correlated with the OSI (p = 0.04). The present results suggest that a higher calcium intake contributes to bone strength in older Japanese males. Although a higher grip strength may contribute to bone strength in females, the potential of estrogen as a confounding factor needs to be considered. 相似文献