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91.
5-氟尿嘧啶缓释剂瘤内注射治疗胰腺癌的实验研究 总被引:8,自引:0,他引:8
目的:观察5FU缓释剂瘤内注射对裸鼠胰腺癌肿瘤细胞的影响,探讨其作用机制。方法:体外培养胰腺癌细胞株PC3,以2×106个细胞分别接种于70只裸鼠。4周后挑选肿瘤大小一致的裸鼠60只,随机分成5组,即静脉NS对照组、5FU静注组(10mg/kg)、基质植入组、5FU缓释剂(4mg/kg)植入组及5FU缓释剂(1mg/kg)植入组。于治疗前及治疗后14d内测量肿瘤大小,计算肿瘤生长速度;观察组织学变化和细胞分裂指数;免疫组化法测定bcl2和Bax的蛋白表达水平;采用脱氧核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测凋亡指数(AI)。结果:5FU缓释剂瘤内注射组裸鼠移植瘤生长速度减慢(P<0.05),最终瘤重小于其他各组(P<0.05);细胞分裂指数亦均低于其他各组(P<0.05)。5FU缓释剂瘤内注射组肿瘤组织中炎症反应和血管内膜增厚程度明显高于其他各组(P<0.05)。5FU缓释剂瘤内注射组荷瘤裸鼠的bcl2基因蛋白表达明显低于其他各组,而Bax基因的蛋白表达明显高于其他各组,其肿瘤细胞的AI明显高于其他各组(P<0.05)。结论:5FU缓释剂瘤内注射可明显抑制裸鼠胰腺癌瘤体的生长,其作用机制与药物在肿瘤组织中引起的炎症反应和血管内膜增厚等因素有关,并可能与诱导肿瘤细胞的凋亡有关。 相似文献
92.
Hy De Lee Euy Young Soh Hoon Sang Chi Byong Ro Kim Kyong Sik Lee Kyung Soon Song Hyun Joo Jung 《Surgery today》1990,20(2):180-185
The relationship between primary tumor proliferative activity and clinical and pathologic characteristics was analyzed in
relation to menopausal status in 32 patients with malignant or benign breast disease. The thymidine labeling index (TLI) showed
significantly higher median values in the cancer patients (3.48 per cent) than in the patients with benign diseases (1.02
per cent). TLI was not significantly affected by delayed incubation at room temperature for about 1 hour. In the breast cancer
patients, TLI did not significantly correlate to tumor size, the presence of axillary lymph node metastasis or pathologic
nuclear grading. The only significant difference was limited to the breast cancer patients without axillary lymph node metastasis
in relation to menopausal status; the TLI in the premenopausal patients (5.10 per cent) was significantly higher (p<0.05)
than that in the postmenopausal patients (2.28 per cent). These data thus suggest that among premenopausal patients without
axillary lymph node metastasis, those with a high TLI could be potential candidates for adjuvant chemotherapy. 相似文献
93.
为探讨仅表达非系限性分化抗原的急性白血病的细胞起源,采用单克隆抗体免疫酶标和聚合酶链反应技术分析了12例初诊时仅表达非系限性分化抗原的急性白血病化疗后免疫表型及免疫球蛋白重链(IgH)和T细胞受体(TCR)γ基因重排的变化。结果表明:初诊时仅表达CD38抗原的5例急性白血病,化疗后3例出现T细胞相关抗原表达,并伴TCRγ基因重排;5例初诊时仅表达HLA-DR或CD9的急性白血病,化疗后4例出现B细胞相关抗原表达,均伴有IgH基因重排;2例无任何抗原表达者,化疗后1例表达B细胞相关抗原,另1例表达骨髓细胞相关抗原。提示免疫标志的动态研究,有助于初诊时免疫学无法分类急性白血病细胞起源的确定。 相似文献
94.
Farida Daïkha-Dahmane Françoise Narcy Marc Dommergues Mireille Lacoste Agnes Beziau Marie-Claire Gubler 《Pediatric nephrology (Berlin, Germany)》1997,11(3):267-273
An alteration in cell/matrix interactions is one of the suggested mechanisms leading to cyst formation in polycystic kidney
diseases. Most of these interactions are mediated by β1-integrins, a subfamily of integrin receptors, formed by the association
of the β1-chain with different α-subunits. To date, no study on α-integrin subunit distribution during the early stages of
cyst development has been reported. Using immunofluorescence, we analyzed the distribution of α-integrin subunits (α1, α2,
α3, α5, and α6) and basement membrane proteins in kidneys of fetuses with autosomal dominant (ADPKD) or autosomal recessive
polycystic kidney disease (ARPKD). The distribution was compared with that observed in normal fetal and post-natal kidneys,
and in fetal cystic dysplasia and Meckel syndrome. Marked increase in α1-integrin staining was observed in normal and cystic
collecting duct cells of both polycystic diseases (PKD), compared with normal and cystic controls. The distribution of integrin
subunits α2, α3, and α6 was irregular in cyst epithelial cells of PKD and cystic controls. The increased expression of the
α1-subunit specifically observed in PKD collecting duct cells may be an early consequence of the genetic defect in ARPKD.
In ADPKD it parallels the reported expression of polycystin, the protein product of PKD1. The irregular expression of α2,
α3, and α6 integrin subunits observed in all types of cysts suggests that cell/matrix interactions are altered early and may
participate in the development of cysts, perhaps by contributing to the deregulation of cell survival in cystic diseases.
Received May 28, 1996; received in revised form October 2, 1996; accepted October 25, 1996 相似文献
95.
本文描述了姜根茎腐烂的三种症状类型:青枯型、黄腐型及黄枯型;分别由青枯细菌、腐霉菌及青桔细菌和腐霉苗引起,鉴定出4种致病腐霉:P(?)(?)P.(?)P.(?)和P.(?).通过接种试验,证明腐霉对姜有较强的致病力;与青枯细菌共同为害,病将更严重,故姜的根茎腐烂不完全是细菌青枯病。 相似文献
96.
Liver transplantation for chronic hepatitis B with lamivudine-resistant YMDD mutant using add-on adefovir dipivoxil plus lamivudine. 总被引:5,自引:0,他引:5
Chung Mau Lo Chi Leung Liu George K Lau See Ching Chan Irene O Ng Sheung Tat Fan 《Liver transplantation》2005,11(7):807-813
Lamivudine treatment in patients with chronic hepatitis B virus (HBV) infection may improve clinical state and suppress viral replication before liver transplantation. Emergence of lamivudine-resistant YMDD mutant is common. We report the results of liver transplantation in 16 patients with pretransplantation YMDD mutants after receiving lamivudine treatment for a median of 738 days (range, 400-1799 days). Adefovir dipivoxil (10 mg daily) was added on to lamivudine for a median of 20 days (range, 8-271 days) before (n = 11) or at (n = 5) liver transplantation, and the combination was continued indefinitely thereafter. Eight patients received additional intravenous hepatitis B immune globulin (HBIG) for a median of 24 months. Fifteen patients with known pre-adefovir HBV DNA levels had a median titer of 14,200 x 10(3) copies/mL (2 x 10(3) to 4,690,000 x 10(3) copies/mL), and 14 had HBV DNA >10(5) copies/mL. All but 1 patient remained positive for HBV DNA (by quantitative polymerase chain reaction [qPCR]) at the time of liver transplantation, and the titer was greater than10(5) copies/mL in 8 patients. The median follow-up after liver transplantation was 21.1 (range, 4.4-68.9) months. One patient (6%) died of an unrelated cause 12.2 months after transplantation, and 15 patients (94%) were alive with the original graft. All patients cleared HBV DNA and had no detectable HBV DNA by qPCR at the latest follow-up. Fourteen patients had cleared hepatitis B surface antigen (HBsAg), but 2 patients who received only adefovir dipivoxil and lamivudine without HBIG remained HBsAg positive after 7.7 and 9.5 months. Serum HBV DNA, however, was negative, and there was no biochemical or histological evidence of recurrence. Adefovir dipivoxil was well tolerated with no significant renal toxicity. In conclusion, a combination of add-on adefovir dipivoxil plus lamivudine therapy provides effective prophylaxis in patients with pretransplantation YMDD mutant that may be actively replicating. The cost effectiveness of additional passive immunoprophylaxis remains to be defined. 相似文献
97.
探讨提高人工全膝关节置换术疗效的因素。总结人工全膝关节置换 1 5例 1 7膝 ,其中 OA6膝 ,RA3膝 ,创伤性关节炎 1膝 ,选用髁式表面假体 ;骨肿瘤 5膝 ,术后失败翻修 2膝 ,选用绞链式假体。结果 :短期随访显示功能良好 ,疼痛消失 ,全部重返生活和工作 ;KSS评分绞链膝 84~ 92分 ,平均 88分 ;表面膝 85~ 94分 ,平均 89分。结论 :只要选择合适的病例 ,设计合理的假体 ,人工全膝关节置换效果非常肯定 相似文献
98.
99.
人正常下颌升支区应力分布的三维有限元法分析 总被引:3,自引:0,他引:3
目的:对正常人下颌升支在不同咀嚼力作用时应力分布情况进行三维有限元分析。方法:采用三维有限元应力分析的方法,对正常离体人下颌升支在不同加载条件下的应力分布特征进行对比定量分析。结果:(1)所有载荷形式的各种应力值,随着单元距加载点的距离的增大,其应力值相对减小。(2)下颌骨下颌角、升支后缘的应力梯度变化较大。结论:下颌骨下颌角、升支后缘是应力相对集中的区域,也是临床上常见的下颌骨骨折区,同时还是下颌升支矢状骨劈开术后加强固定以及防止骨段移位的区域。 相似文献
100.