The Effect of Aging on Erectile Function Induced by Apomorphine and Electric Field Stimulation to Rat

Population aging has become a world wide tendency and according to the UNproject,the proportion of population above60 years old would exceed the proportionof children below1 5years old by2 0 50 .Erectile dysfunction(ED) seriously affectsthe quality of lif…     

Effects of basic fibroblast growth factor on biological characteristics of osteoblasts

Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro. Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF ( 5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 ( TGF-β1 )was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Resu/ts: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β1 mRNA increased significantly, but the intracellular ALP content decreased. Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.     

癌基因c—erb B—2在结肠血吸虫病并发肠癌和肠息肉中的表达及AgNOR观察

探讨肠道血吸虫病与肠癌发生的关系。应用免疫组织化学ＬＳＡＢ法结肠血吸虫病并发结肠癌，肠息肉和无血吸虫病的肠部，肠息肉行ｃ－ｅｒｂＢ－２标记并ＡｇＮＯＲ染色。结果；肿瘤细胞中癌基因ｃ－ｅｒｂＢ－２表达呈明显异质性；血吸虫病并发肠癌与无血吸虫病的肠癌表达阳性率，两者之间无明显；血吸虫病肠息肉的表达明显高于无血吸虫病的肠息肉。     

带锁髓内钉固定并植骨治疗陈旧性股骨干骨折

目的 评价带锁髓内钉并植骨治疗股骨干陈旧性骨折的临床疗效和优点。方法 对1996年2月～2003年11月收治的47例陈旧性股骨干骨折患者进行回顾性分析。其中植骨组28例，非植骨组19例。采用切开复位，带锁髓内钉扩髓，静力型固定，根据骨折部位生长情况改为动力型固定。结果 术后随访39例，随访lO～25个月，平均随访时间14．2个月。植骨组骨折愈合率为95．6％(22／23)，感染率为4．3％(1／23)，骨折平均愈合时间为6．3个月，非植骨组则分别为62.5%(10／16)，6．25％(1／16)和9．7个月。临近关节功能达正常，无感染、断钉等并发症。结论 带锁髓内钉静力固定能有效控制有害的剪应力，同时对骨折有一定的加压作用，可提供早期坚强的内固定，同时，植骨后骨折愈合快，并发症少，是治疗陈旧性骨折的理想方法。     

经肝动脉与门静脉同时插管灌注化疗治疗晚期肝癌

<正> 1988年5月至2000年5月我们对不能手术切除的31例晚期肝癌患者经肝动脉与门静脉同时插管、间断灌注化疗,临床观察治疗效果尚满意,现总结报告如下。 1 临床资料 1.1 一般资料:31例中男27例,女4例,年龄最小37岁,最大68岁,平均年龄54例。原发性肝癌24例,弥漫性肝癌7例,均经病理诊断证实。 1.2 辅助治疗:为完成整个治疗过程,减少化疗药物抑制造血系统的毒性作用,保护消化功能。本组在化疗灌注的前、中、后同时应用以下辅助治疗:①聚肌胞2ml肌注或静滴2～3次/周。②干扰素1支,肌注1～3次/周。③华蟾素10～30mg     

跨供区髂骨皮瓣一期修复肢体大面积骨与软组织缺损

Objective To study the clinical effect of iliac flap straddled donor area to primary repair large defect of bone and soft tissue. Methods Through the anatomical study about iliac flap, the new oper-ation method about iliac flap was designed, which straddled donor area and used one blood vessel stem (deep iliae circumflex artery) and two branch blood vessel(deep iliac circumflex artery and superficial iliac circum-flex artery compounding in series) for blood supply. Blood supply area of the analogical operation was stud-ied by irrigating fresh cadaver with Indian ink. Thirty-two patients with large defect of bone and soft tissue (20 males, 12 females, 17-68 years old, average 36 years old) were repaired by this method, and the hemo-dynamics was studied by laser Doppler flowmetry. Results It was feasible to connect superficial iliac cir-cumflex artery with the branch of deep iliac circumflex artery. Indian ink irrigating fresh cadaver simulate operation showed that area scope dyed by Indian ink: in ilium, Indian ink was found in the bone which was (15.8±3.2) cm away from anterior superior iliac spine. In skin flap, Indian ink was found (8.6±2.4) cm superi-or, (5.4±2.6) cm inferior, (5.8±2.6) cm internal and (24.6±5.8) cm external from the anterior superior iliac spine. Laser Doppler flowmetry showed that the peripheral blood flow of skin flap increased as time passed, the increase speed began to tardy at the third day, then ingress the platform stage. Thirty-two cases were all succeed with functional rehabilitation and perfect shape. Thirty-one cases got completely bone healing; bone healing time ranged from 9 to 19 months, with an average of 12 months. One case turned to chronic os-teomyelitis, and had to suffer operation again, the bone healing time was 22 months. Conclusion As the ili-ae flap straddled donor area has sufficient blood supply, it can repair large defect of bone and soft tissue in one stage.     

足背动脉跨区供血的足背及足底内侧联合皮瓣的解剖研究与临床应用

目的探讨应用同一血管蒂（足背动脉）跨供区（足背与足底内侧）联合皮瓣游离移植的可行性。方法对足背和足底内侧2个皮瓣进行解剖学研究。采用10只成人新鲜足标本分别从足背动脉（5只）、足底内侧动脉（5只）加压灌注碳素墨汁甲醛。在足底和足背不同部位取全层皮肤做脱水透明标本处理，在显微镜下对0．3-0．5mm厚铺片进行观察，以确定不同动脉的供血范围。设计以足背动脉供血的跨足背与足底内侧2个皮瓣供区的新皮瓣移植术式，并用来修复手部大面积皮肤缺损。结果足背皮瓣与足底内侧皮瓣存在丰富的交通血供区，从足背动脉灌注碳素墨汁甲醛，其分布范围除整个足背外，在足底分布如下：以足底中线纵轴为基线可及中线外侧（1．7±0．5）cm；以足底中线横轴为基线，上可及中线上（4．5±1．5）cm，下可及中线下（3．8±0．9）cm。从足底内侧动脉灌注碳素墨汁甲醛，其分布范围除整个足底内侧区域外，在足背区可及躅长伸肌外侧（0．6±0．5）cm。以足背动脉供血的跨区联合皮瓣血供丰富，临床应用12例全部成功。结论以足背动脉供血的跨区联合皮瓣切取面积大，血供可靠，可以用来修复手部大面积皮肤缺损。     

心脏外科手术中胃粘膜pH的变化

了解心脏直视手术时胃粘膜ＰＨ的变化规律及意义。方法选择１５例心脏直视手术病人，手用经鼻置入附有胃内二氧化碳张力计套囊的胃管，测量手术中及术后ＰＨｉ值，同时观察血液动力学及氧合状态各指标变化。     

基于OBE教育理念的康复医学理论课教学改革研究

本文基于成果导向教育（outcome-based education,OBE）理念,在理论课教学中以学生为中心,通过组建助教团队、强化课程思政、优化课程内容设计,在教学方法与教学平台上进行变革,并通过增强教学反馈力度等方式,从多个维度和方面探索出以学生成长成才为目的,知识能力、价值观同发展的育人模式。融合OBE教育理念的康复医学课程,效果优于往年传统康复医学教学模式,学员满意度、接受度、认同度超过95%,且期末成绩优良率占比高于去年同门课程。该模式下的教学改革初探为提高康复医学教学质量和培养康复医学现代化专业型人才提供了借鉴意义。     

心房颤动治疗的未来发展方向——混合消融

心房颤动是临床常见的心律失常,已有研究证明其与严重不良心脑血管事件(心力衰竭、脑卒中和心肌梗死)有关,目前全球心房颤动的患病人数超过了3 300万,预计未来40年内其患病率将增加1倍以上。多年来,医学相关人员在探究心房颤动的病理生理机制及开创改进其治疗方法等方面付出了大量努力。目前心房颤动的治疗管理仍是临床医学上的一个难题,尽管心房颤动治疗的手术消融和导管消融技术已逐渐趋于成熟,但对于心房颤动最佳的治疗方式、消融能量的选择尚无统一定论。导管消融通常需要多次手术且成功率低,而手术消融术后不良事件发生率较高。近年来,鉴于心脏外科医生和电生理学家之间的密切合作,结合导管及微创手术消融诞生了一种治疗心房颤动的新型策略——混合消融模式。混合消融克服了导管消融和微创手术消融的缺点,减少了不良结局,在治疗持续性心房颤动,尤其是长期持续性心房颤动上取得了可观的成效。本文主要通过回顾心房颤动消融的研究进展,对比分析目前混合消融模式治疗心房颤动的现有研究成果,归纳总结这种新型心房颤动治疗策略的优势与挑战,以期为临床心房颤动的治疗提供更多选择。