THERPEUTIC EFFECTS ON EXPERIMENTAL METASTIC TUMOR- BEARING MICE BY QI-LE(启乐) POLYSACCHARIDE ANTITUMOR METASTASES

Objective: To study the efficiency and the mechanism of QI-LE polysaccharide (QI-LE), a new compound extracted from one kind of Chinese herbs with antiplatelet activity, against tumor metastasis in mice. Methods: QI-LE was prepared by our Lab. C57BL/6 female mice transplanted with H₂₂ liver cancer cells were administered with the agent. Then the tumor nodules, pulmonary metastasis rate, and tumor cell proliferation activity were checked. Proliferation changes of tumor cells treated with different concentrations of QI-LE were also tested. Results: High and middle dosage of QI-LE could inhibit both the lymphatic and blood systemic tumor pulmonary metastases significantly, but not the tumor cell proliferation in vitro. Conclusion: QI-LE has antitumor metastasis activity, thus may be a new candidates for antitumor metastases agents. Foundation item: This work was supported by the Science Foundation from Health Department of Shandong Province (No. 2001CAICDAI) and Chinese Medicine Administration Bureau of Shandong Province(No. 99-46). Biography: Zheng Guang-juan(1964–), female, doctor of medicine, associate professor, Shandong University of Traditional Chinese Medicine, majors in tumor pathology and immunology.     

益气活血复方脂血康抗大鼠动脉内皮损伤作用的实验研究

脂血康能显著降低３６周高胆固醇膳食所致高脂血症动物血浆总胆固醇（ＴＣ）、低密度脂蛋白胆固醇（ＬＤＬｃ）和ＬＰＯ的水平，升高高密度脂蛋白胆固醇（ＨＤＬｃ）的水平，增加血中ＧＳＨ－Ｐｘ和ＳＯＤ的活性，防止血管内皮细胞损伤及平滑肌细胞的增殖和内迁。提示该复方可通过对血浆脂蛋白和氧自由基代谢的影响而预防ＡＳ病变的形成     

Experiment studies of antitumor proliferation and metastasis of a new chinese herb AT-1

Objective: To study the effects of a new Chinese herb AT-1 on the tumor cell proliferation and metastasis in vitro.Methods: Tumor cell proliferation activity was tested by MTT. The ability of tumor cell invasion and migration was assayed by counting the number of tumor cells going throw matrigel. The expression changes of CD44 genes in PG cells treated with AT-1 were tested by FACS. Results:Compared with the control, the proliferation activity of the cells treated with the At-1 was restrained. The invasion and migration ability of PG cells and the expression of the cell adherence related gene CD44 was decreased treatment with AT-1. Conclusion: AT-1 is a new antitumor proliferation and metastasis agent. Its antitumor metastasis effect might be achieved by decreasing the expression of the cell adherence-associate gene CD44.     

HCA520, a novel tumor associated antigen,involved in cell proliferation and apoptosis

Objective: Tumor associated antigen encoding gene HCA520 (AF146019) was identified by screening a human hepatocellular carcinoma expressing cDNA library using SEREX technique. In this experiment we studied the effect of HCA520 on cell proliferation and apoptosis.Methods: Gene HCA520 was gained by PCR and transfected into 293 cells. The stable expression cells were obtained by G418 selection. The cell proliferation was measured by [^3H]-TdR uptake and apoptosis assay was measured by FACS. Results: Eukaryotic expression plasmid pcDNA3-HCA520 was constructed and its stable transfectants were obtained. Overexpression of HCA520 inhibited the cell proliferation and enhanced cell apoptosis after serum deprivation. Conclusion: HCA520 is a novel tumor associated antigen that can affect cell proliferation and apoptosis.     

丹参对烯丙醇小鼠肝损伤修复过程中AgNORs的动态影响

用烯丙醇制成大鼠肝损伤模型，观察了丹参在损伤修复过程中对细胞核内ＡｇＮＯＲｓ数目的动态影响。结果显示：与模型对照组相比，丹参可使损伤周围残存的肝细胞内的ＡｇＮＯＲｓ数目很快增多，坏死区很快由再生的肝细胞填充，而较少形成纤维性肉芽组织     

体外罗勒多糖抗人高转移肺癌细胞侵袭转移作用及机制探讨

目的：体外观察罗勒多糖对人高转移肺癌细胞侵袭、转移行为的影响，并探讨其可能的作用机制。 方法： 在体外侵袭模型中观察不同浓度罗勒多糖对人高转移肺癌细胞侵袭、转移行为的影响；划痕标记染料示踪技术检测罗勒多糖对细胞通讯功能的影响；RT-PCR检测肿瘤细胞内转移相关分子c-myc、nm23-H1与Tiam-1 mRNA表达水平的改变。 结果： 罗勒多糖处理组细胞的侵袭与转移能力明显低于对照组（P<0.05）；细胞通讯功能有一定程度的恢复，荧光染料传递毗邻3-4列细胞；细胞内促进肿瘤细胞转移的基因c-myc、Tiam-1表达程度低于对照组，而抑制肿瘤细胞转移的基因nm23表达程度高于对照组（P<0.05）。 结论： 罗勒多糖是一种具有抗人高转移肺癌细胞侵袭以及转移作用的新药，它的作用是通过多个药效靶点综合作用的结果。     

四步法中医实验教学

目前,中医教育中普遍存在重理论、轻实践,重知识传授、轻能力和素质培养的现象,这对学生创新能力的培养和个性的发展极为不利.在实验教学方面,按照传统的教学模式,以课堂为中心,以教师为中心,验证性实验比重大且部分内容陈旧,学生依赖思想严重且对实验课教学兴趣不高.这些都限制了学生合理的知识结构和智力结构的建立,不仅不利于知识的学习和掌握,而且浪费了培养和提高学生科研技能的机会.面对世界范围内日趋激烈的科技竞争尤其是人才竞争的严峻现实,如何培养既有中医传统文化底蕴,又具创新能力的中医科研人才,是中医实验教学研究的首要内容.     

补肾化瘀解毒方荷瘤药物血清对A549/DDP耐药逆转作用的研究

目的 :为了探讨补肾化瘀解毒方对肺癌耐药的逆转作用 ;方法 :选择人肺腺癌耐药细胞株 A5 4 9/DDP为模型 ,采用荷瘤动物血清药理及体外细胞毒试验方法 ;结果 :补肾化瘀解毒方荷瘤动物含药血清对肺癌耐药细胞 A5 4 9/DDP有抑制作用 ,并能增强 A5 4 9及 A5 4 9/DDP对化疗药物 DDP的敏感性 ;结论 :表明补肾化瘀解毒方荷瘤药物血清对化疗药物具有增敏和化疗耐药逆转作用     

降脂红曲微粉治疗高脂血症临床研究

目的:观察不同剂量降脂红曲微粉治疗高脂血症的降脂疗效和安全性,为降脂红曲的临床应用和开发提供科学依据。方法:80例高脂血症患者随机分为4组,A组(降脂红曲微粉1/2剂量组),B组(降脂红曲微粉组),C组(降脂红曲粗粉组)和D组(血脂康组)。观察治疗前后总胆固醇(TC)、三酰甘油(TG)、脂蛋白(a)[Lp(a)]、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、载脂蛋白A-I(ApoA-I)、载脂蛋白B100(ApoB100)等指标的变化。结果:用药50 d后,各组患者TC、Lp(a)水平、LDL-C/HDL-C比值、ApoB100/ApoA-I比值显著降低,ApoA-I水平显著升高,P<0.01或P<0.05;降脂红曲微粉1/2剂量组还显著降低TG,降脂红曲微粉1/2剂量组、粗粉组与血脂康组还能显著降低LDL-C,P<0.01。各组对血脂异常均有较好疗效,降脂红曲微粉1/2剂量组、血脂康组总有效率优于降脂红曲粗粉组,P<0.05。结论:降脂红曲微粉降脂疗效肯定,安全,剂量可以较常规粉碎者减半,有推广价值。     

虎杖苷对颈动脉粥样硬化斑块稳定性的干预研究

目的 观察虎杖有效成分虎杖苷对颈动脉粥样硬化及其斑块稳定性的干预作用.方法 将64例颈动脉粥样硬化患者按随机数字表法分为治疗组32例和对照组32例.治疗组给予虎杖苷胶囊口服,每次1粒,每日2次;对照组给予洛伐他汀片口服,每次20mg,每日1次.两组患者均连续治疗6个月.采用高分辨率超声技术观察治疗前后颈动脉内膜-中膜厚度(IMT)、斑块Crouse积分及颈动脉斑块超声病理分型改变;同时检测治疗前后血清基质金属蛋白酶-1(MMP-1)及其抑制物(TIMP-1)水平变化.结果 与治疗前比较,治疗组颈动脉IMT与斑块积分显著减小(P＜0.05,P＜0.01),血清MMP-1、MMP-1/TIMP-1水平显著降低,不稳定斑块的软斑数量有减少趋势,但无统计学意义(P＞0.05).治疗组对上述指标的作用与对照组比较,差异无统计学意义.结论 虎杖有效成分虎杖苷具有抗动脉粥样硬化作用,并有助于稳定斑块,值得进一步研究.