全文获取类型
收费全文 | 2609篇 |
免费 | 99篇 |
国内免费 | 64篇 |
学科分类
医药卫生 | 2772篇 |
出版年
2024年 | 15篇 |
2023年 | 46篇 |
2022年 | 53篇 |
2021年 | 35篇 |
2020年 | 55篇 |
2019年 | 58篇 |
2018年 | 81篇 |
2017年 | 36篇 |
2016年 | 47篇 |
2015年 | 55篇 |
2014年 | 140篇 |
2013年 | 166篇 |
2012年 | 167篇 |
2011年 | 190篇 |
2010年 | 201篇 |
2009年 | 169篇 |
2008年 | 158篇 |
2007年 | 162篇 |
2006年 | 129篇 |
2005年 | 100篇 |
2004年 | 103篇 |
2003年 | 116篇 |
2002年 | 90篇 |
2001年 | 63篇 |
2000年 | 37篇 |
1999年 | 74篇 |
1998年 | 35篇 |
1997年 | 22篇 |
1996年 | 20篇 |
1995年 | 40篇 |
1994年 | 25篇 |
1993年 | 19篇 |
1992年 | 15篇 |
1991年 | 11篇 |
1990年 | 18篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有2772条查询结果,搜索用时 218 毫秒
132.
133.
134.
135.
136.
体检人群食物不耐受血清特异性IgG检测 总被引:1,自引:1,他引:0
目的初步分析体检者食物不耐受情况,为疾病的预防提供依据。方法选取103例住院体检者,采用酶联免疫吸附法(ELISA)检测血清中食物过敏原特异性IgG浓度并加以比较。结果在日常食用的14种食物中,不耐受率从0%~47.6%,特异性抗体浓度升高排列在前几位的分别是虾(47.6%)、鸡蛋(30.1%)、蟹(27.2%)。食物不耐受程度:患慢性病者较健康者重,老年组较中青年组,女性较男性严重。结论人群存在食物不耐受现象,食物过敏原特异性抗体测定有助于食物引起过敏性疾病的早期预防和诊断。 相似文献
138.
Objective To evaluate the therapeutic effects of recombinant expression plasmid containing hepatocyte growth factor (HGF) and augmenter of liver regeneration (ALR) on rats with hepatic fibrosis. Methods Ninety Sprague-Dawley rats, which had been established into hepatic fibrosis models, were equally divided into 6 groups: blank group, pcDNA3.1 therapy group,pcDNA3.1-HGF therapy group, pcDNA3. 1-ALR therapy group, pcDNA3.1-HGF and pcDNA3. 1-ALR combined therapy group, and pcDNA3. 1-HGF-ALR therapy group. Zero point one μmol of blank or plasmid was injected into model rats in each group by tail vein once a day for 3 days. Model rats in blank group didn't receive any treatment. Additional 10 rats were chosen as control group, which were not given any interference during the experiment. All rats were sacrificed 4 days after end of treatment. Liver tissues were reserved for observing pathologic changes after HE staining and detecting proliferating cell nuclear antigen (PCNA) and c-jun by immunohistochemistry. Measurement data were compared by single-factor analysis of variance. Comparison between groups was done by SNK test. Enumeration data were analyzed by Fisher's exact test. Results In blank group and pcDNA3.1 therapy group, hyperplasia of fibrous connective tissue was very obvious, false lobules were formed. There was no significant difference between these two groups (x2 =0. 317,P= 1. 000).In the 4 remaining groups, hepatic fibrosis all achieved different degree of amelioration, and the therapeutic effect of pcDNA3.1-HGF-ALR was optimal. In control group, the expressions of PCNA and c-jun in liver tissues were low, with absorbance value of 8.6±1.9 and 3.2 ± 1.2, respectively. In blank group and pcDNA3. 1 therapy group, the expressions of PCNA and c-jun were obviously increased, with absorbance value of 24. 1±3.0, 24.5±4.3 and 23.8±3.1, 24.9±4.2, respectively,which were significant different from control group (all P<0.01). In the 4 remaining groups, the expressions of PCNA were all obviously increased, and expressions of c-jun were all obviously decreased. The maximum change scope was observed in pcDNA3. 1-HGF-ALR therapy group.Conclusions The recombinant expression plasmid pcDNA3. 1-HGF-ALR can effectively ameliorate experimental hepatic fibrosis of rats. The anti-fibrosis effects are achieved probably by up-regulating PCNA expression and down-regulating c-jun expression. 相似文献
139.
140.
<正>患者男,49岁。入院10d前无明显诱因出现右上腹疼痛,疼痛放射至右肩背部。入院后经检查诊断为急性结石性胆囊炎。行腹腔镜胆囊切除术(LC),取出胆囊内结石1枚,约1.5cm×2cm,术后安返病房。于术后24h出现胸闷、气促、 相似文献