miR-186上调Dicer1抑制结肠癌细胞侵袭转移的分子机制

为探讨miR-186通过Dicer1影响结肠癌细胞侵袭转移及其作用机制,RT-qPCR检测miR-186在结肠癌组织及正常癌旁组织、人结肠癌细胞及正常人肠上皮细胞HIEC中的表达;荧光显微镜观察稳定过表达/下调miR-186细胞系GFP荧光并采用RT-qPCR检测miR-186表达效率;Transwell及划痕实验检测miR-186对结肠癌细胞SW620和HT29侵袭、迁移能力的影响;免疫荧光法检测上皮间质转化(epithelial mesenchymal transition, EMT)相关分子N-cadherin表达;生物信息学预测及双荧光素酶报告基因实验验证miR-186和Dicer1的靶向关系;免疫组织化学法检测Dicer1在结肠癌组织及正常癌旁组织中的表达;Western blotting检测Dicer1在各细胞株中的表达。结果显示,肿瘤组织中miR-186的表达水平显著低于正常癌旁组织(P0.05);miR-186在肿瘤细胞中的表达水平显著低于HIEC(P0.05)。转染过表达/下调miR-186慢病毒阳性质粒及阴性对照的SW620和HT29细胞均出现大量绿色荧光;RT-qPCR显示稳定过表达细胞SW620-mimic-miR-186中miR-186水平显著升高,HT29-inhibitor-miR-186中miR-186水平显著下降,分别与SW620和SW620-mimic-NC、HT29和HT29-inhibitor-NC相比差异有统计学意义(P0.05)。过表达miR-186能显著降低SW620的侵袭迁移能力,同时抑制N-cadherin水平,而下调miR-186能显著增加HT29的侵袭迁移能力。经www.microRNA.org网站预测发现miR-186和Dicer1有互补结合序列,双荧光素酶报告实验证实两者的靶向结合关系。SW620中Dicer1表达水平显著低于HIEC(P0.05);稳定过表达细胞系SW620-mimic-miR-186中Dicer1表达水平明显升高,分别与SW620和SW620-mimic-NC相比差异有统计学意义(P0.05)。由此,miR-186通过靶向正调控Dicer1的表达降低N-cadherin水平从而抑制EMT进程,最终抑制结肠癌细胞的侵袭迁移能力。     

自拟生肌镇痛膏治疗肛门术后疼痛1300例

肛门病术后疼痛问题，一直是肛肠学科研究课题。近１０年止痛研究多反映在改进手术方法和局部长效麻醉液的研究上，而外用药物相对较少，笔者近１０年来，着重外用药的研究，曾几易配方，制成生肌镇痛膏，获得显著止痛效果，现报告如下：临床资料：本组１３００例中，男８...     

吃出来的“疵瑕”好无奈

蝴蝶斑对人的整体美造成了损害,当你皮肤上出现了令人讨厌的蝴蝶斑时,不要只怨天忧人,从自己的饮食中,你也许能查出个中原委。     

长期服用马利兰致肺纤维化1例

长期服用马利兰致肺纤维化１例晋红梅，王克强马利兰是慢性粒细胞白血病的常用化疗药物，长期服用导致肺纤维比１例，现报告如下。患者男，５０岁，３年前无明显诱困发现左上腹出现包块，且迅速增大，并出现双下肢浮肿。２年前左上腹包块继续增大，占据整个左侧腹部，双下...     

男性慢性精神分裂症恢复期患住院依赖行为因素分析及对策的研究

住院慢性精神分裂症患者,由于多种因素可对住院产生不同程度的依赖性,本研究着重探讨了通过系统护理干预手段,对住院依赖行为的作用效果.2003年1月-2004年1月,对我院住院精神分裂症男性患者100例进行了临床研究,取得了较好的临床效果,现报道如下.     

脐静脉内皮细胞及其培养液中血栓素测定的初步探讨

研究人脐静脉内皮细胞能否分泌血栓素及妊高征患者血清对其分泌活动的影响。方法体外原代培养５例妊高征患者ＨＵＶＥＣ和５例正常妊ＨＵＶＥＣ，每例分为３组，当其融合时分与妊高征患者和正常孕妇的产前血清以及培养液共同孵育２４ｈ。     

可怕的隐形杀手

生活中一些意想不到的事件,在引起无法挽回的错误的同时,也应引起我们的注意,毕竟,这是用生命换来的经验,倾听专家的解释更重要的是不要重蹈覆辙。     

放疗中呼吸运动对不同形状射野剂量分布的影响

Objective To evaluate the influence of respiration on the radiation dose distribution within target volume in radiotherapy with film dosimetry. Methods Radiation of 50 MU was delivered by a square, round, ellipse, dumb bell, or female shaped filed to the films within a moving or motionless Respiration Motion Phantom respectively, the dose distributions for the two motion status were measured and compared. In order to further verify the impact of respiration, a plank phantom was used on different shifting value: 0, 0.5, 1.0, 1.5 and 2.0 cm, respectively. A square, round, or eUipse-shaped filed was used for irradiation and the distributions in different status were measured and compared with film dosimetry. Iso-dose line comparison, NAT(Normalized Agreement Tests) and γ comparison were used for the comparison of dose distributions. Fs can be an index to reflect the variability of the areas that surrounded by iso-dose lines. (FS90, FS50, FS25 delegates the ratio of the areas that surrounded by 90 %,50 %,25 % iso-dose hne in different situation respectively). Results (1) Compared with motionless situation, the FS90 in horizontal movement situation became small and the FS25 became large. As the displacement became larger, the FS90 became larger and the FS25 became smaller. FS in vertical movement situation, square and dumb bell fields changed while the others didn't have a change. (2)γ and NAT comparison: In the horizontal movement situation, compared with the static phantom, Pγ < 60 % and PNAT < 75 %. Under vertical movement situation, Pγ were less than 85 % for the square, round, dumb bell and female shaped fileds. In the plank phantom verification, Pγ and PNAT became smaller as the movement became larger. Conclusions The respiration can impact on the dose distribution within the target volume in radiotherapy, leading to a smaller area of higher dose level and an expanded area of lower dose level. The influence will become more significant with larger movement of the target.