术中剩余皮肤组织匀浆在网状皮移植中的应用

烧伤临床上进行皮肤移植手术时,因为术者对创面大小估计的误差、取皮质量、移植过程中皮片裁剪等因素,创面被充分覆盖后常有剩余供皮.为了充分利用这部分皮肤,我们将其制作为混悬匀浆,与网状皮肤混合移植于创面,观察其疗效.     

医疗卫生工作者代表向全市广大医疗卫生工作者倡议：一辈子当一个好医生

全市医疗卫生工作： 学习华益慰精神，加强医德医风建设，提高医疗服务水平，真正把我市医疗卫生行业建设成为全心全意为人民服务，让人民群众放心和满意的行业。为此，我们参加丹东市卫生局学习华益慰精神。加强医德医风建设座谈会的医疗卫生工作代表，向全市广大医疗卫生工作发出倡议：     

复方乙呋甲麻油膏治疗老年性阴道炎的临床观察

目的:使用复方乙呋甲麻油膏治疗老年性阴道炎的临床治疗及疗效观察。方法:观察组阴道灌洗加局部用复方乙呋甲麻油膏,对照组口服乙烯雌酚加阴道内放置双唑泰泡腾片。结果:用药10天后,观察组97.3%的患者症状消失,治愈率达90.0%,而对照组症状消失率76.4%,治愈率75.0%。两组治愈率差异有显著性(P<0.05)。结论:复方乙呋甲麻油膏治疗老年性阴道炎,设计合理,取材普遍,配置简单,使用方便,疗效可靠,患者乐于接受,值得基层尤其是农村医疗单位推广。     

温阳益气方对脾肾阳虚型便秘疗效和生存质量的影响

目的:探讨温阳益气方对脾肾阳虚型便秘患者临床症状及生存质量的影响。方法:采用随机、对照的临床试验方法,治疗组和对照组各纳入24例患者,疗程为4周,观察两组治疗前后PAC-QOL积分及症状评分的变化。结果:温阳益气方治疗组总有效率为70.83%,莫沙必利组总有效率为41.60%;两组疗后PAC-QOL积分均较治疗前降低;治疗组在躯体不适、担心与焦虑和满意度3个方面及总分方面与对照组比较,有统计学差异(P<0.05);症状评分比较方面,治疗组各组症状评分均较治疗前下降,有统计学差异(P<0.05);两组治疗后比较,其中治疗组对排便费力程度和排便时间的改善程度优于对照组(P<0.05)。结论:温阳益气方临床治疗脾肾阳虚型便秘有较好的疗效,能明显改善脾肾阳虚型便秘患者临床症状,提高生存质量。     

灌注法构建小肠黏膜全层去细胞外基质

背景：对于解剖层次复杂的细胞外基质,单纯浸泡法则很难达到理想的去细胞效果。 目的：观察灌注法去细胞技术制备小肠黏膜全层去细胞外基质的效果。 方法：采用灌注法去细胞技术,经成年雄性新西兰大白兔小肠黏膜上动脉灌注去离子剂后处理获得小肠 黏膜全层去细胞外基质。采用MTT法检测小肠黏膜全层去细胞外基质浸提液(实验组)或含体积分数20% 小牛血清的DMEM(对照组)与1 月龄新西兰大白兔骨髓间充质干细胞共培养后的细胞相对增殖率。 结果与结论：①大体观察：小肠黏膜上动脉经灌注30 min 后变得苍白透明,经2 h 灌注后肠段变得苍 白剔透,清楚可见脉管纹理。②组织学与扫描电镜观察：灌注法构建的小肠黏膜全层去细胞外基质的去 细胞程度均匀,胶原纤维未受损,孔隙多,孔隙率为(86.72±2.98)%。③MTT 检测实验：培养2,4,7 d, 实验组细胞A 值明显高于对照组细胞A值(P < 0.05),且实验组细胞相对增殖率均>1。表明采用灌注法 构建小肠黏膜全层去细胞外基质可操作性强,安全性好,且小肠黏膜全层去细胞外基质对骨髓间充质干 细胞无毒性,并有一定的促生长作用。     

重组沙门菌表达结核分枝杆菌ESAT-6及其特异性免疫应答分析

Objective To determine the immune responses induced by recombinant Salmonella ty-phimurium expressing the secreting antigen ESAT-6 of Mycobacterium tuberculosis. Methods ESAT-6 cod-ing gene was cloned and identified by PCR and sequencing. Prokaryotic expression plasmid pYA33-esat car-rying the ESAT-6 coding sequence was constructed firstly and electro-transformed into an attenuated strain X4550 of Salmonella typhimurium, the recombinant bacteria was named as X4550(33-esat). C57BL/6 mice were immunized intranasally (I. N) with 108 CFU recombinant bacteria at day 0 and 18. Cells from spleen, lung, mesenteric lymph node (MLN) and Peyer's patch (PP) were collected from mice after second immu-nization, and the specific IFN-γ-secreting cells and IL-4-secreting cells were detected by ELISPOT assay u-sing ESAT-6 peptide as stimulus. Furthermore, CTL effects were in vivo evaluated by CFSE assay. Results The results showed that cellular immune responses specific for ESAT-6 could be detected by ELISPOT assay. In lung and PP cells, immune responses against ESAT-6 were biased toward Th1 type, the frequency of IFN-γ-secreting cells was much higher than that of IL-4-secreting cells. In splenocytes and MLN cells, the anti-gen specific immune responses acted as Thl and Th2 balance, the frequency of IFN-γ-secreting cells was close to that of IL-4-secreting cells. CFSE assay indicated that recombinant bacteria could induce the high level of CTL effects specific for ESAT-6 peptide. Conclusion These results suggested that recombinant Sal-monella typhimurium X4550(33-esat) not only can induce cellular immune responses, but also can elicit specific CTL responses after I. N immunization. It also provided the useful information for the control of infec-tious disease of tuberculosis.     

重组沙门菌表达结核分枝杆菌ESAT-6及其特异性免疫应答分析

Objective To determine the immune responses induced by recombinant Salmonella ty-phimurium expressing the secreting antigen ESAT-6 of Mycobacterium tuberculosis. Methods ESAT-6 cod-ing gene was cloned and identified by PCR and sequencing. Prokaryotic expression plasmid pYA33-esat car-rying the ESAT-6 coding sequence was constructed firstly and electro-transformed into an attenuated strain X4550 of Salmonella typhimurium, the recombinant bacteria was named as X4550(33-esat). C57BL/6 mice were immunized intranasally (I. N) with 108 CFU recombinant bacteria at day 0 and 18. Cells from spleen, lung, mesenteric lymph node (MLN) and Peyer's patch (PP) were collected from mice after second immu-nization, and the specific IFN-γ-secreting cells and IL-4-secreting cells were detected by ELISPOT assay u-sing ESAT-6 peptide as stimulus. Furthermore, CTL effects were in vivo evaluated by CFSE assay. Results The results showed that cellular immune responses specific for ESAT-6 could be detected by ELISPOT assay. In lung and PP cells, immune responses against ESAT-6 were biased toward Th1 type, the frequency of IFN-γ-secreting cells was much higher than that of IL-4-secreting cells. In splenocytes and MLN cells, the anti-gen specific immune responses acted as Thl and Th2 balance, the frequency of IFN-γ-secreting cells was close to that of IL-4-secreting cells. CFSE assay indicated that recombinant bacteria could induce the high level of CTL effects specific for ESAT-6 peptide. Conclusion These results suggested that recombinant Sal-monella typhimurium X4550(33-esat) not only can induce cellular immune responses, but also can elicit specific CTL responses after I. N immunization. It also provided the useful information for the control of infec-tious disease of tuberculosis.