散发性结直肠癌CpG岛甲基子表型和基因组遗传学不稳定性的关系

目的探讨散发性结直肠癌CpG岛甲基子表型和基因组不稳定性的关系。方法对采用甲基化特异性PCR的方法对71例散发性结直肠癌组织进行P14^ARF、hMLH1、P16^INK4a、MGMT和MINT1共5个基因启动子甲基化的检测,确定CpG岛甲基子表型;选择BAT25和BAT26两个位点进行微卫星不稳定检测和流式细胞术检测分析倍体类型;分析散发性结直肠癌中CpG岛甲基子表型和微卫星不稳定、染色体不稳定的关系。结果全组结直肠癌组织中CpG岛甲基子表型的阳性率为21.1%（15/71）;微卫星不稳定的阳性率为9.9%（7/71）;异倍体的阳性率为73.5%（50/68）。CpG岛甲基子表型阳性者,微卫星不稳定的阳性率高于阴性者（20.0%vs7.1%）,但差异无统计学意义（P=0.158）。hMLH1基因启动子甲基化阳性者微卫星不稳定的比例为57.1%,高于阴性者的4.7%（P=0.001）。CpG岛甲基子表型阳性者二倍体的比例高于阴性者（61.5%vs.18.2%,P=0.003）。结论CpG岛甲基子表型阳性的散发性结直肠癌具有显著的二倍体倾向,多基因同时甲基化和染色体不稳定可能是两种相互独立的基础性发病机制。     

补阳还五汤对早期糖尿病肾病患者血清C-反应蛋白及PAI-1的影响

[目的]观察补阳还五汤对早期糖尿病肾病患者血清C-反应蛋白(C-reactive protein,CRP)及血浆纤溶酶原激活物抑制因子-1(plasminogen activator inhibitor-1,PAI-1)水平的影响.[方法]将60例早期糖尿病肾病患者随机分为治疗组和对照组各30例,2组均用西药常规治疗,...     

三种定位方法在腹腔镜结直肠肿瘤手术中的应用效果

目的评价术前亚甲蓝定位、金属夹定位和术中纤维结肠镜定位在腹腔镜结直肠肿瘤手术中的应用效果。方法复旦大学附属肿瘤医院2009年12月至2012年6月间收治的64例结直肠肿瘤患者在行腹腔镜手术前进行了肿瘤定位,其中术前2h内4点法亚甲蓝定位23例,术前1d金属夹定位20例,术中纤维结肠镜定位21例,定位后行腹腔镜结直肠肿瘤根治性手术、肠段切除或局部切除术。结果所有手术均获成功,无手术死亡和并发症发生。术前亚甲蓝定位标记成功率为82．6％（19／23）,2例因亚甲蓝弥散导致组织界限不清行中转开腹手术,2例肿瘤因腹腔面肠壁无亚甲蓝显色而无法定位,遂于术中加行纤维结肠镜定位。术前金属夹定位标记成功率为85．0％（17／20）,2例乙状结肠肿瘤和1例直肠上段肿瘤因无法确定下切缘而于术中加行纤维结肠镜定位。术中肠镜定位标记成功率为95．2％（20／21）,1例因病灶为长蒂腺瘤未能准确定位。对于术前亚甲蓝和金属夹定位失败而加行术中结肠镜定位的5例患者中,2例准确定位并成功施行腹腔镜手术;1例因病灶为长蒂腺瘤未准确定位;2例定位准确但因小肠和结肠胀气明显,手术空间不足致中转开腹手术。结论上述3种定位方法用于腹腔镜结直肠肿瘤手术均可获得较为满意的定位效果。临床实践中应根据肿瘤位置和拟行的手术方式来选择适宜的肿瘤定位方法。     

四妙散加味结合西药治疗痛风性关节炎69例疗效观察

目的：观察四妙散加味对痛风性关节炎的临床疗效。结论：四妙散加味配合西医常规治疗痛风性关节炎,疗效满意。     

香烟烟雾诱导小鼠气道炎症中的差异甲基化基因筛选及功能分析

目的 构建香烟烟雾诱导的小鼠气道炎症模型,筛选其炎症反应过程中的差异甲基化基因,并分析基因功能,为深入探讨甲基化修饰在慢性阻塞性肺疾病中的调控作用提供实验依据.方法 选取20只雄性C57BL/6小鼠随机分为对照组和熏烟组,每组各10只.分别暴露于过滤空气和香烟烟雾连续4周(2小时/次,2次/天,6天/周).4周后获取小...     

Ab-externo precise localization of Schlemm’s canal in patients with primary open-angle glaucoma北大核心

Objective To investigate the ab-externo approach for the precise localization of the Schlemm’s canal (SC) in patients with primary open-angle glaucoma (POAG). Methods A total of 40 POAG patients (40 eyes) who underwent ab-externo SC-related surgeries in Weifang Eye Hospital from August 2020 to October 2021 were selected. According to the intraoperative measuring method, the patients were randomly divided into the high-definition (HD) image group and the sterile drawing group, with 20 patients (20 eyes) in each group. In the HD image group, HD images of the surgical area were captured by a microscope for measurement of the distance between loci. In the sterile drawing group, the loci were marked on the sterile drawing with a mapping compass under the microscope before measurement of their distance. The boundary line between the semitransparent and non-transparent tissues was marked by the sclerotic scatter and used as the base line (BL). A blood reflux band of SC was found on the scleral bed by the transillumination in the anterior chamber with the fiber optic endoscope. The outer wall of SC was removed completely during the operation for measuring related indexes, such as the width of SC (SW), the distance between the BL and the posterior edge of SC (d1), the distance between the conjunctival insertion line (CIL) and the anterior edge of SC (d2), and the distance between the BL and the CIL (d3). The white-to-white (WTW) distance and axial length (AL) were measured with IOL-Master before surgery. Differences and correlations among these indexes were analyzed. Results d2 [(1.85±0.28) mm] and d3 [(2.63±0.30) mm] in the HD image group were higher than those [d2: (1.61±0.37) mm and d3: (2.41±0.38) mm] in the sterile drawing group, and the differences were statistically significant (both P<0.05). WTW and d1 were positively correlated with AL (both P<0.05), d1 and d2 were positively correlated with d3 (both P<0.05) in both groups, while the rest indexes had no significant correlation (all P>0.05). The 40 eyes were divided into 3 groups according to AL: short AL group (AL<23 mm), medium AL group (23 mm≤AL<25 mm), and long AL group (AL≥25 mm). d1 in the short, medium, and long AL groups were (0.30±0.08) mm, (0.37±0.10) mm, and (0.57±0.09) mm, respectively, and the differences among the three groups were statistically significant (F=14.915, P<0.05). d1 in the long AL group was longer than that in the short and medium AL groups (both P<0.05). Conclusion The transillumination system can clearly display the SC blood reflux band on the deep scleral bed, facilitating the precise localization of SC. d1 is positively correlated with AL in POAG patients. © The Author(s) 2023.