成功抢救重度异烟肼中毒致心脏停跳1例体会

1临床资料 患者,男,17岁,入院31小时前自服异烟肼约100片(10g).服药后约20分钟出现意识障碍,全身抽搐.急送当地医院急救.治疗期间发生3次心跳骤停,每次持续时间约2分钟.     

简易鼻塞CPAP治疗新生儿呼吸衰竭（附25例报告）

新生儿呼吸衰竭是新生儿疾病的重要死亡原因之一 ,缺氧和二氧化碳潴留可造成多脏器损伤 ,因此临床上必须采取快速而有效的方法缓解缺氧 ,我们对 2 5例新生儿呼吸衰竭给予经鼻呼吸道持续正压通气 ( CPAP)治疗 ,取得了良好效果 ,现分析如下 :1　临床资料1 .1　一般资料　 2 5例病人均为住院的危重新生儿 ,新生儿呼吸衰竭的诊断标准根据 1 986年 9月全国新生儿学术会议 (杭州 )拟定新生儿呼吸衰竭诊断标准的初步方案[1] ,其中 1 0例经血气分析符合 型呼吸衰竭 ,Pa CO2 <70 mm Hg,且呼吸节律规则 ,呼吸频率在 40～ 80次 /min,除外中枢性呼…     

Differentiation of human epidermal stem cells into fibroblasts induced by TGF-β1 in vitro

Objective To investigate the correlation between human epidermal stem cell (hESCs) and hypertrophic scar or keloid. Methods Improved collagen Ⅳ-coated adhesion methods was used to isolate and culture the epidermal stem cells after neutral protease selectively digested the dermo-epidermal junctions. After the cells were cultured and expanded in vitro, and passage 3 hESCs were induced by different concentrations of TGF-β1 (0.1, 5.0, and 10.0 ng/ml). Morphological fea-tures and identification of these cells were meseasured by HE, Masson, immunohistochemical staining on the days 3 and 7, respectively. Results After induced by TGF-β1 for 3 and 7 days, the morpholo-gy of the epidermal stem cell (hESCs) was changed into fusiform shape, similar to fibroblasts. 70 % ofthe cell which was induced by TGF-β1 were blue stained in the cytoplasm by Masson stain, which is the distinctive method for collagen, suggesting collagen appeared or increased in the cells. The collagen concentrations in supernatants of hESCs were 0.4150±0.0014, 0.3380±0. 0020, and 0.3870±0.0020, much higher than that in control group (0.0780±0.0025) and normal skin fibro-blast group (0.15004±0.0051) (P<0.05). Immunohistochemical staining revealed that positive rates of these cells for anti-vimentin staining were more than (95.00±1.20)% in experiments and (5.70±0.20)% in control group. Conclusion The differentiantion of hESCs induced by TGF-β1 into fibro-blasts indicates that hESCs may play a role in the pathogenesis of hypetrophic scar and keloid.     

大剂量甲基强的松龙冲击疗法治疗急性视神经炎临床疗效观察

目的：评估大剂量甲基强的松龙冲击疗法治疗急性视神经炎的疗效． 方法：应用大剂量甲基强的松龙冲击疗法治疗急性视神经炎30例．并与常量地塞米松疗法进行了病例对照观察。 结果：治疗组与对照组比较篷异有显著性(P<0.01)，治疗组见效快、副作用少、安全可靠。 结论：大剂量甲基强的松龙冲击疗法是治疗急性视神经炎的更为有效方法． （中华眼底病杂志，1996，12：186-187）     

牛角角蛋白与人角质层抗原检测抗角质层抗体的比较研究(摘要)

本文以牛角角蛋白和人角质层为抗原,用生物素——亲和素酶联免疫吸附试验(BA—ELISA)检测了50名银屑病患者和32名正常人血清中抗角质层抗体(ACAS 的滴度,虽然以牛角角蛋白作抗原     

Propofol and Sevoflurane in Subanesthetic Concentrations Act Preferentially on the Spinal Cord: Evidence from Multimodal Electrophysiological Assessment

Background: Animal experiments in recent years have shown that attenuation of motor responses by general anesthetics is mediated at least partly by spinal mechanisms. Less is known about the relative potency of anesthetic drugs in suppressing cortical and spinal electrophysiological responses in vivo in humans, particularly those, but not only those, connected with motor responses. Therefore, we studied the effects of sevoflurane and propofol in humans using multimodal electrophysiological assessment.

Methods: We studied nine healthy volunteers in two sessions during steady state sedation with 0.5, 1.0, and 1.5 [mu]g/l (targeted plasma concentration) propofol or 0.2 and 0.4 vol% (end-tidal) sevoflurane. Following a 15-min equilibration period, motor responses to transcranial magnetic stimulation and peripheral (H-reflex, F-wave) stimulation were recorded, while electroencephalography and auditory evoked responses were recorded in parallel.

Results: At concentrations corresponding to two thirds of C50 awake, motor responses to transcranial magnetic stimulation were reduced by approximately 50%, H-reflex amplitude was reduced by 22%, F-wave amplitude was reduced by 40%, and F-wave persistence was reduced by 25%. No significant differences between sevoflurane and propofol were found. At this concentration, the Bispectral Index was reduced by 7%, and the middle-latency auditory evoked responses were attenuated only mildly (Nb latency increased by 11%, amplitude PaNb did not change). In contrast, the postauricular reflex was suppressed by 77%.     

人工全髋关节置换术围手术期护理体会

目的:总结全髋关节置换术围手术期的护理体会.方法:对18例人工全髋关节置换术患者采用整体化护理模式,制定切实可行的护理计划,认真做好术前、术后护理工作及功能锻炼.结果:本组18例术后恢复快,效果满意,无1例因护理不当而引起并发症,均在2～3周出院.结论:全髋关节置换术围手术期高质量护理与手术成功密不可分.