基于UHPLC-QTOF-MS/MS和分子对接筛选紫菀中润肠通便的效应

目的 通过UHPLC-QTOF-MS/MS和分子对接技术阐明紫菀Aster tataricus中润肠通便作用的效应成分。方法 采用UHPLC-QTOF-MS/MS技术分析体内肠道内容物的成分,再利用SYBYL-X 2.0与Discovery Studio 4.0分子对接软件研究肠道内容物成分与M2受体、M3受体、蛋白激酶C(protein kinase C,PKC)蛋白的相互作用,明确各成分与靶标蛋白的结合强度。结果 体内肠道内容物中共鉴定出28个紫菀中的化学成分,有10个成分均可与M2受体、M3受体及PKC蛋白分子对接较好,其中astin J与3种靶标蛋白结合的平均打分值最高,这些成分均以非共价键方式与靶标蛋白结合,产生氢键、范德华力、经典作用力等相互作用。结论 紫菀中的astin J、asterin F、asterin A、异绿原酸A、异绿原酸B、异绿原酸C、绿原酸、槲皮素、山柰酚和木犀草素共10个成分可能是通过调节M受体及其下游信号通路PKC蛋白的表达发挥润肠通便的作用。     

乳化剂OP存在下胶束增溶分光光度法测定水中镉

目的：用胶束增溶分光光度法测定水中镉的含量,并比较了乳化剂OP(聚乙二醇辛基苯基醚)和吐温-20(聚氧乙烯山梨醇酐单月桂酸酯)的胶束增溶作用。方法：分别以体积分数为10％OP和体积分数为10％吐温-20为增溶剂,以PAN(1-(2-吡啶偶氮)-2-萘酚)为显色剂,在555nm波长处测定水中镉络合物的吸光度。结果：相同条件下,同浓度的镉络合物用OP的吸光度值是吐温-20的1．6倍,用OP作乳化剂平均回收率为96．5％,平行样品的相对平均偏差为2．57％,标准曲线线性良好,回归系数r=0．999 9,ε=4．6×104L·mol-1·cm-1。结论：乳化剂OP的增溶作用优于吐温-20,此法测定水中痕量镉较好。     

误诊文献的特点及开发价值

近年来,有关报道临床误诊误治的文献量明显增加,经人工检索１９９０～１９９７年２００多种临床医学期刊,发现误诊文献５万篇。误诊文献具有真实性、类似性及可借鉴性等特点。抓住并利用这些特点,在对数万份误诊文献统计处理后,从看似无序的误诊现象中挖掘出了其中蕴...     

肌无力综合征1例分析

1 病例介绍 患者,男,61岁。因咳嗽、胸闷、憋气2个月于2002年6月2日收入院。病人2个月前出现咳嗽,咳少量白痰,伴胸闷、憋气,活动后加重,无痰中带血,无发热及盗汗,无胸痛,抗炎治疗效果不佳。作胸部CT示:左肺下叶背段见约7cm×8cm大小肿块,密度不均,左肺门淋巴结肿大,局部胸膜增厚。入院查体:一般情况尚可,前胸部皮下及左侧第8肋间、腋后线处分别扪及1.5cm×1.5cm、3cm×3cm皮下结节,质硬,无触痛。左下肺呼吸音低,     

HPLC测定人血清中利福平的浓度

目的　建立测定血清中利福平浓度的高效液相色谱法 ,并应用于临床。方法　采用Nova -pakC18色谱柱 (4 μm ,15 0nm× 3.9mm) ,以甲醇 - 0 .0 2mol·L-1醋酸钠 (6 2∶4 0 )为流动相 ,紫外检测波长 334nm ,测定口服利福平 4d以上的患者血清中利福平的浓度。结果　血清中利福平在 0 .5～ 16 .0 μg·ml-1范围内浓度与峰面积线性关系良好 (r=0 .9992 ) ,平均回收率为99 .4 3% ,日内、日间RSD <5 .0 %。结论　所用方法准确、灵敏 ,用血量少 ,适用于治疗药物监测 ,为临床用药提供数据参考     

对无助急诊患者开辟绿色通道

所谓“无助患者”是指患者来院就诊时无家属、亲戚、朋友的陪伴 ,且当时无能力支付医疗费用[1] 。我院本着“一切以患者为中心 ,优质服务 ,奉献爱心”的服务宗旨 ,以“维持人们的健康是医药卫生人员的光荣使命”为导向 ,为无助急诊患者开辟了一条绿色通道 ,全面实施一条龙服务 ,取得了满意的效果 ,得到了社会舆论的公认和褒奖。1　临床资料1 1　一般资料　 2 0 0 0年 1月— 2 0 0 1年 12月共收治无助急诊患者 13 2例 ,其中男 114例 ,女 18例 ;年龄 19～ 67岁。入院途径 :由 12 0救护车送入医院的 5 7例 ,由 110巡警或 12 2交通清障车送入医…     

针刺四关穴治疗偏头痛及其对一氧化氮水平的影响

目的：观察针刺四关穴治疗偏头痛的疗效及其对血浆一氧化氯(N0)水平的影响。方法：对38例偏头痛患者以针刺四关穴治疗，并于治疗前后作血浆N0水平测定。结果：经治疗，患者总有效率78．95％；治疗后血浆N0水平明显升高。结论：四关穴针刺治疗偏头痛有确切疗效，其机理可能与升高血浆N0水平有关。     

卵巢外腹膜浆液性乳头状癌(附8例分析)

1959年Swerdlow首次报道卵巢外腹膜浆液性乳头状癌(extraovarian pexitoneal serous pillary carcinoma，EPSPC)。由于对起源有争议，临床上少见，文献报道中该肿瘤名称甚多，给诊断和治疗带来困难，且常误诊为卵巢癌盆、腹腔内广泛转移或恶性间皮瘤。我们回顾性分析了我院1988年3月至2002年4月收治EPSPC8例，现报告如下。     

51例乳腺癌患者血清可溶性Fas(sFas)水平变化的探讨

目的　探讨乳腺癌患者血清 s Fas水平变化的意义。方法　采用 EL ISA法检测血清 s Fas水平。结果　乳腺癌组血清 s Fas水平明显高于正常对照组 (P<0 .0 1) ;随着乳腺癌病情的发展 ,s Fas水平有增高趋势 ; 期乳腺癌患者血清 s Fas水平明显高于其它各期 (P<0 .0 1) ;乳腺癌手术后并化疗者较乳腺癌手术前血清 s Fas水平有明显下降 (P<0 .0 5 )。结论　血清 s Fas与乳腺癌的发生和发展有密切联系 ,检测血清 s Fas水平可作为乳腺癌诊断的辅助检查。     

转染CD40配体的卵巢癌细胞株OVHM抗卵巢癌肝转移机制的研究

Objective To examine the in vivo anti-metastatic effect of enhanced expression of CD40L cDNA in murinc ovarian cancer OVUM cells (CD4OL-OVHM) injected into the spleen on liver metastasis in mice. Methods OVUM cells were inoculated into the spleen of 6 ～ 8 week-old female B6C3F1 (C57BL/6N × C3H/He) mice. The established liver metastasis was identified by histopathology (HE staining). OVUM cells, DNA-pMKITneo-OVHM cells or CD40L-OVHM cells were inoculated into the spleen of female B6C3F1 mice and the expressions of CD11c in splenic cells were detected by flow cytometry. The specific cytotoxicity of splenic cells was detected by MTT assay, and the serum cytokines of IFN-γ, TNF-α, IL-12, IL4 and IL-10 of the mice were measured by enzyme linked immunoabsorbent assay. The liver metastases and the survival time of the mice were also recorded. Results The mouse models with liver metastasis by injecting tumor cells into the spleen of mice were established. The expression of CD11c and the specific killing rote in CD40L-OVHM cells group was significantly higher than that in the OVHM cells group and DNA-pMKITneo-OVHM cells group. The expressions of IFN-γ, TNF-α and IL-12 in the CD40L-OVHM cells group were much more increased than OVHM cells group and DNA-pMKITneo-OVHM cells group, but the expressions of IL-4 and IL-10 in the CD40L-OVHM cells group were decreased significantly (P < 0.05). The average weights of livers and spleens of mice in CD40L-OVHM cells group were significantly lower than those of DNA-pMKITneo-OVHM cells group and OVHM cells group. The survival time of mice in CD40L-OVHM cells group was also significantly longer than that in the OVHM cells group and DNA-pMKITneo-OVHM cells group (P < 0.05). Conclusion The data directly demonstrate that the expression of CD40L in ovarian cancer cells (CD40L-OVHM) can enhance the proliferation and differentiation of dendritic cells in the spleen and induce specific cytotoxic effect of T cells in the spleen, and may regulate the immune function of peripheral blood cells and the immune balance between Thl cells and Th2 cells, which maybe the possible mechanism induced by CD40L in mice inhibiting the development of liver metastasis.