Superlocalization spectral imaging microscopy of a multicolor quantum dot complex

The key factor of realizing super-resolution optical microscopy at the single-molecule level is to separately position two adjacent molecules. An opportunity to independently localize target molecules is provided by the intermittency (blinking) in fluorescence of a quantum dot (QD) under the condition that the blinking of each emitter can be recorded and identified. Herein we develop a spectral imaging based color nanoscopy which is capable of determining which QD is blinking in the multicolor QD complex through tracking the first-order spectrum, and thus, the distance at tens of nanometers between two QDs is measured. Three complementary oligonucleotides with lengths of 15, 30, and 45 bp are constructed as calibration rulers. QD585 and QD655 are each linked at one end. The measured average distances are in good agreement with the calculated lengths with a precision of 6 nm, and the intracellular dual-color QDs within a diffraction-limited spot are distinguished.     

直驱转台伺服系统的优化神经网络滑模位置控制研究

针对直驱转台伺服系统存在参数变化、高度非线性、机械谐振等不确定性因素,提出一种优化神经网络滑模位置控制方法。利用两个神经网络控制器分别实现转台系统滑模控制中的等效滑模控制和滑模切换控制,并采用遗传算法优化神经网络的权值。仿真结果表明:采用该控制方法,直驱转台伺服系统的位置跟踪精度高,系统的鲁棒性强。     

桩墩扶壁复合土钉墙工作性状数值分析

针对软土地区采用桩墩扶壁复合土钉墙围护结构的基坑,按实际工况进行了开挖支护的三维非线性有限元模拟,其中土体采用修正剑桥弹塑性模型,计算结果与现场实测数据能较好地吻合,表明本文研究方法是可行的。现场实测数据与四种不同型式复合土钉墙围护结构基坑开挖的数值计算结果,均表明三维基坑开挖存在明显的“角部效应”;在复合土钉墙中设置灌注桩墩与锚杆对于减小灌注桩、搅拌桩、锚杆、土钉及周边土体变形及内力有是利的,且灌注桩墩的影响效应要超过锚杆,故在深基坑开挖中采用桩墩扶壁复合土钉墙支护结构是合理有效的。     

On the database-based strategy of candidate extractant generation for de-phenol process in coking wastewater treatment

A database-based strategy of candidate generation was proposed for molecular design of new de-phenol extractants following the idea of finding new applications of existing commercial compounds. The strategy has the advantage that the environmental, safety and health risks of candidate compounds are known and controllable.In this work, the Existing Commercial Compounds (ECC) database and special combined search strategy were developed as the base for the proposed CAMD method following such idea, and molecules for phenol extraction used in coking wastewater treatment were selected from the ECC database. The candidate solvents cover the following categories: ketones, esters, ethers, alcohols, anhydrides and benzene compounds, which are consistent with the de-phenol extractants commonly used in the industry or experiment. The compounds with higher partition coefficient and selectivity than widely used methyl isobutyl ketone (MIBK) are mainly ketones. 26 obtained molecules showhigher partition coefficient and selectivity than MIBK,which are suggested to be further investigated by experiment. Furthermore, analysis of these potential moleculesmay present the effective functional groups as the initial group set to generate newmolecular structures of de-phenol extractants. The results showthat the proposedmethod enables us to efficiently generate chemicals with benefits of less time, less economical cost, and known environmental impact as well.     

西门子TDC控制系统在双机架可逆冷轧中的应用

本文介绍了浙江龙盛薄板有限公司1500mm双机架可逆冷轧生产线自动化控制系统的构成和实际运行效果及功能特点,并取得了很好的控制效果。     

基于PLM的制造企业应用系统集成的研究

异构系统间的信息的交换与共享在制造业信息系统集成中有着重要的作用,PLM系统是制造企业信息化工程的重要集成平台。通过分析CAX、PLM与ERP系统集成的必要性及集成时的要点,针对各自系统的特点,提出了一种基于PLM的CAX、PLM与ERP的集成方法,采用Web Service作为集成的技术支撑平台,利用XML文件作为中间转化格式,来实现系统的信息资源的交换与共享,以达到资源的合理化利用。     

基于磁性共价有机骨架对烤肉中苯并[a]芘的检测

采用快速简单的共沉淀法合成磁性共价有机骨架材料,并通过傅里叶变换红外光谱、透射电镜对其进行表征.通过优化吸附剂用量、萃取时间、解吸溶剂、解吸时间和解吸体积等条件,建立了磁固相萃取-高效液相色谱法测定烤肉中苯并[a]芘(Benzo[a]pyrene,BaP)的新方法.该方法的线性范围为0.5～100.0 μg/L(R2=...     

纳米复合材料Ag/TiO2微波辅助合成与光催化性能

采用EO20PO70EO20(P123)作为模板剂,通过溶胶-凝胶-程序升温溶剂热一步法并经不同时间微波辐射处理,制备了一系列纳米复合材料Ag/TiO2。采用XRD、XPS、TEM、N2吸附-脱附测定和SEM-EDS等测试手段对其组成、结构及形貌等进行表征。结果显示,微波辐射5min制备的Ag/TiO2晶型结构最佳,该产物中Ag以单质形式存在,其比表面积可达100.64m2·g-1,平均孔径约为6.9nm。与未经微波辐射样品相比,其颗粒细小、结构规则、分布均匀、无明显团聚现象。以甲基橙为模型分子,考察经不同时间微波辐射处理的纳米复合材料Ag/TiO2的紫外光催化活性。结果表明,在微波功率为200W,微波时间为5min时获得的Ag/TiO2的催化活性最高,循环使用3次后降解率仍能达到80%以上。     

大型复杂悬臂结构铝合金焊接变形控制研究

研究的结构件为大型复杂悬臂结构铝合金焊接件,因其既有环焊缝又有纵焊缝,焊缝数量多,焊接变形量大,焊接变形趋势复杂,使得通常的工艺加工方法很难满足其设计性能要求。通过对影响焊接变形的几个因素进行详细分析,选择合理的焊接方法、焊接参数,采取有效的工艺措施,通过试验进行验证,圆满地解决了这一难题,此工艺方案完全适用于类似大型悬臂结构件的焊接。     

Graphene oxide nanoribbon and polyhedral oligomeric silsesquioxane assembled composite frameworks for pre-concentrating and electrochemical sensing of 1-hydroxypyrene

1-Hydroxypyrene (1-OHP) is a ubiquitous metabolite of polycyclic aromatic hydrocarbons (PAHs) and has been widely used as a biomarker for evaluation of human exposure to PAHs. In this paper, an electrochemical sensor consisted of graphene oxide nanoribbon (GON)/octa(3-aminopropyl)octasilae quioxane octahydrochloride (OA-POSS) framework films (GON/OA-POSS) modified electrode for pre-concentrating and sensing of 1-OHP was described. The GON/OA-POSS composite films were prepared by hierarchically layer-by-layer assembly via electrostatic interactions. GON, which has higher electrical conductivity than that of graphene oxide, offers residual π-conjugated systems for adsorption of 1-OHP via π–π stacking interaction. Meanwhile, OA-POSS nanocages make the GON layers frameworked to enhance contacting between GON and 1-OHP. When 1-OHP was pre-concentrated on GON/OA-POSS frameworks modified electrode, the response signal and adsorption stability was enhanced significantly. Differential pulse voltammetry was used for direct determination of 1-OHP. The electrochemical sensor calibration curve had a range of 0.1–12.55 μM (R = 0.995) with a detection limit of 0.04 μM. The results showed that the GON/OA-POSS framework films modified electrode established a new way for simple and sensitive analysis of 1-OHP.