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991.
Nadia Ouabadi Véronique Gauthier‐Brunet Thierry Cabioc'h Guo‐Ping Bei Sylvain Dubois 《Journal of the American Ceramic Society》2013,96(10):3239-3242
Reactive sintering of 3Ti:Sn:2C and 3Ti:Sn:2C:0.6Fe powder mixtures is studied in the temperature range 510°C–1200°C under argon. It is demonstrated that the recently discovered Ti3SnC2 phase is formed, provided that Fe is added to a 3Ti:Sn:2C reactant mixture within the synthesis conditions used. Using dilatometric and X‐Ray diffraction analyses, the formation mechanism of Ti3SnC2 is discussed. Results show that at low temperature (about 510°C), tin is consumed to form FexSny intermetallics. At high temperature (about 1060°C), tin is newly available to form Ti3SnC2 due to the melting of FexSny. Then, the intermediate phases, TiC and Ti2SnC, and/or Ti5Sn3, TiC, C, and Ti are dissolved in the (Fe + Sn) liquid phase and Ti3SnC2 very likely precipitate from the melt. The second part of the study deals with the optimization of the Fe content in the initial 3Ti:Sn:2C reactant powder mixture to synthesize samples with larger Ti3SnC2 content by hot isostatic pressing. 相似文献
992.
J. Michael Conlon Laura K. Reinert Milena Mechkarska Manju Prajeep Mohammed A. Meetani Laurent Coquet Thierry Jouenne Marc P. Hayes Gretchen Padgett-Flohr Louise A. Rollins-Smith 《Journal of chemical ecology》2013,39(6):797-805
Population declines due to amphibian chytridiomycosis among selected species of ranid frogs from western North America have been severe, but there is evidence that the Oregon spotted frog, Rana pretiosa Baird and Girard, 1853, displays resistance to the disease. Norepinephrine-stimulated skin secretions were collected from a non-declining population of R. pretiosa that had been exposed to the causative agent Batrachochytrium dendrobatidis. Peptidomic analysis led to identification and isolation, in pure form, of a total of 18 host-defense peptides that were characterized structurally. Brevinin-1PRa, -1PRb, -1PRc, and -1PRd, esculentin-2PRa and -PRb, ranatuerin-2PRa, -2PRb, -2PRc, and -2PRe, temporin-PRb and -PRc were identified in an earlier study of skin secretions of frogs from a different population of R. pretiosa known to be declining. Ranatuerin-2PRf, -2PRg, -2PRh, temporin-PRd, -PRe, and -PRf were not identified in skin secretions from frogs from the declining population, whereas temporin-PRa and ranatuerin-2PRd, present in skin secretions from the declining population, were not detected in the current study. All purified peptides inhibited the growth of B. dendrobatidis zoospores. Peptides of the brevinin-1 and esculentin-2 families displayed the highest potency (minimum inhibitory concentration = 6.25–12.5 μM). The study provides support for the hypothesis that the multiplicity and diversity of the antimicrobial peptide repertoire in R. pretiosa and the high growth-inhibitory potency of certain peptides against B. dendrobatidis are important in conferring a measure of resistance to fatal chytridiomycosis. 相似文献
993.
Kedarnath Rane Thierry Barriere Matteo Strano 《The International Journal of Advanced Manufacturing Technology》2020,107(11):4389-4402
The 3D printing of metals and ceramics by the extrusion of a powder/thermoplastic binder feedstock is an extrusion-based additive manufacturing (EAM) techn 相似文献
994.
995.
Tarek Alouane Hlne Rimbert Jrg Bormann Gisela A. Gonzlez-Montiel Sandra Loesgen Wilhelm Schfer Michael Freitag Thierry Langin Ludovic Bonhomme 《International journal of molecular sciences》2021,22(12)
Fusarium graminearum, the primary cause of Fusarium head blight (FHB) in small-grain cereals, demonstrates remarkably variable levels of aggressiveness in its host, producing different infection dynamics and contrasted symptom severity. While the secreted proteins, including effectors, are thought to be one of the essential components of aggressiveness, our knowledge of the intra-species genomic diversity of F. graminearum is still limited. In this work, we sequenced eight European F. graminearum strains of contrasting aggressiveness to characterize their respective genome structure, their gene content and to delineate their specificities. By combining the available sequences of 12 other F. graminearum strains, we outlined a reference pangenome that expands the repertoire of the known genes in the reference PH-1 genome by 32%, including nearly 21,000 non-redundant sequences and gathering a common base of 9250 conserved core-genes. More than 1000 genes with high non-synonymous mutation rates may be under diverse selection, especially regarding the trichothecene biosynthesis gene cluster. About 900 secreted protein clusters (SPCs) have been described. Mostly localized in the fast sub-genome of F. graminearum supposed to evolve rapidly to promote adaptation and rapid responses to the host’s infection, these SPCs gather a range of putative proteinaceous effectors systematically found in the core secretome, with the chloroplast and the plant nucleus as the main predicted targets in the host cell. This work describes new knowledge on the intra-species diversity in F. graminearum and emphasizes putative determinants of aggressiveness, providing a wealth of new candidate genes potentially involved in the Fusarium head blight disease. 相似文献
996.
Laurent Suissa Pavel Kotchetkov Jean-Marie Guigonis Emilie Doche Ophlie Osman Thierry Pourcher Sabine Lindenthal 《International journal of molecular sciences》2021,22(2)
The role of ketone bodies in the cerebral energy homeostasis of neurological diseases has begun to attract recent attention particularly in acute neurological diseases. In ketogenic therapies, ketosis is achieved by either a ketogenic diet or by the administration of exogenous ketone bodies. The oral ingestion of the ketone ester (KE), (R)-3-hydroxybutyl (R)-3-hydroxybutyrate, is a new method to generate rapid and significant ketosis (i.e., above 6 mmol/L) in humans. KE is hydrolyzed into β-hydroxybutyrate (βHB) and its precursor 1,3-butanediol. Here, we investigate the effect of oral KE administration (3 mg KE/g of body weight) on brain metabolism of non-fasted mice using liquid chromatography in tandem with mass spectrometry. Ketosis (Cmax = 6.83 ± 0.19 mmol/L) was obtained at Tmax = 30 min after oral KE-gavage. We found that βHB uptake into the brain strongly correlated with the plasma βHB concentration and was preferentially distributed in the neocortex. We showed for the first time that oral KE led to an increase of acetyl-CoA and citric cycle intermediates in the brain of non-fasted mice. Furthermore, we found that the increased level of acetyl-CoA inhibited glycolysis by a feedback mechanism and thus competed with glucose under physiological conditions. The brain pharmacodynamics of this oral KE strongly suggest that this agent should be considered for acute neurological diseases. 相似文献
997.
Serena Vittorio Thomas Seidel Arthur Garon Rosaria Gitto Thierry Langer Laura De Luca 《International journal of molecular sciences》2021,22(2)
Protein-protein interactions (PPIs) play a pivotal role in the regulation of many physiological processes. The dysfunction of some PPIs interactions led to the alteration of different biological pathways causing various diseases including cancer. In this context, the inhibition of PPIs represents an attractive strategy for the design of new antitumoral agents. In recent years, computational approaches were successfully used to study the interactions between proteins, providing useful hints for the design of small molecules able to modulate PPIs. Targeting PPIs presents several challenges mainly due to the large and flat binding surface that lack the typical binding pockets of traditional drug targets. Despite these hurdles, substantial progress has been made in the last decade resulting in the identification of PPI modulators where some of them even found clinical use. This study focuses on MUC1-CIN85 PPI which is involved in the migration and invasion of cancer cells. Particularly, we investigated the presence of druggable binding sites on the CIN85 surface which provided new insights for the structure-based design of novel MUC1-CIN85 PPI inhibitors as anti-metastatic agents. 相似文献
998.
Rahouti Anass Lovreglio Ruggiero Datoussaïd Sélim Descamps Thierry 《Fire Technology》2021,57(6):3041-3078
Fire Technology - In a healthcare context, the success of a fire safety procedure in a real-life emergency mainly depends on staff decisions and actions. One of the factors influencing staff... 相似文献
999.
Ralfs Buks Mgane Brusson Sylvie Cochet Tatiana Galochkina Bruno Cassinat Ivan Nemazanyy Thierry Peyrard Jean-Jacques Kiladjian Alexandre G. de Brevern Slim Azouzi Wassim El Nemer 《International journal of molecular sciences》2021,22(7)
Myeloproliferative neoplasms (MPNs) are a group of disorders characterized by clonal expansion of abnormal hematopoietic stem cells leading to hyperproliferation of one or more myeloid lineages. The main complications in MPNs are high risk of thrombosis and progression to myelofibrosis and leukemia. MPN patients with high risk scores are treated by hydroxyurea (HU), interferon-α, or ruxolitinib, a tyrosine kinase inhibitor. Polycythemia vera (PV) is an MPN characterized by overproduction of red blood cells (RBCs). ABCG2 is a member of the ATP-binding cassette superfamily transporters known to play a crucial role in multidrug resistance development. Proteome analysis showed higher ABCG2 levels in PV RBCs compared to RBCs from healthy controls and an additional increase of these levels in PV patients treated with HU, suggesting that ABCG2 might play a role in multidrug resistance in MPNs. In this work, we explored the role of ABCG2 in the transport of ruxolitinib and HU using human cell lines, RBCs, and in vitro differentiated erythroid progenitors. Using stopped-flow analysis, we showed that HU is not a substrate for ABCG2. Using transfected K562 cells expressing three different levels of recombinant ABCG2, MPN RBCs, and cultured erythroblasts, we showed that ABCG2 potentiates ruxolitinib-induced cytotoxicity that was blocked by the ABCG2-specific inhibitor KO143 suggesting ruxolitinib intracellular import by ABCG2. In silico modeling analysis identified possible ruxolitinib-binding site locations within the cavities of ABCG2. Our study opens new perspectives in ruxolitinib efficacy research targeting cell types depending on ABCG2 expression and polymorphisms among patients. 相似文献
1000.
Jonathan Raynaud Vincent Pateloup Mégane Bernard Delphine Gourdonnaud Damien Passerieux Dominique Cros Valérie Madrangeas Philippe Michaud Thierry Chartier 《Journal of the European Ceramic Society》2021,41(3):2023-2033
Stereolithography is an additive manufacturing process, which makes it possible to fabricate useful complex 3D ceramic parts with a high dimensional resolution, a good surface roughness and properties close to those obtained by classical routes. Previous work concerning LTCC components, demonstrates that it is possible, by coupling the stereolithography with robocasting additive processes, to obtain multi-material components (e.g. ceramic / metal components). On the base of this previous work, the manufacturing of HTCC components using this innovative hybrid additive manufacturing process is described. Various complex and innovative geometries of HTCC alumina/tungsten components, in order to improve the characteristics of current circuits, are built and the mechanical and electrical properties characterized. Finally, hyper-frequency parameters of simulated HTCC complex micro strip resonators were compared to measured values on components manufactured by additive manufacturing. 相似文献