25 Jahre Fraunhofer IST

25 years Fraunhofer IST — Coatings for our future The Fraunhofer Institute for Surface Engineering and Thin Films IST was founded in Hamburg 25 years ago, it moved to Braunschweig in 1994. Starting with a small group of researchers around Dr. Heinz Dimigen, the IST developed to a service center for problem solutions around the surface. Remarkable research results have been gained in the field of diamond‐like carbon coatings for tribological applications, for large area deposition of polycrystalline diamond, for transparent conductive oxides (TCOs), the metallization of carbonfiber‐reinforced plastic (CFRP) and in precision optics. Emerging topics are surfaces for bio and medicine technology as well as plasma medicine.     

In Vitro Co-Culture Models of Breast Cancer Metastatic Progression towards Bone

Advanced breast cancer frequently metastasizes to bone through a multistep process involving the detachment of cells from the primary tumor, their intravasation into the bloodstream, adhesion to the endothelium and extravasation into the bone, culminating with the establishment of a vicious cycle causing extensive bone lysis. In recent years, the crosstalk between tumor cells and secondary organs microenvironment is gaining much attention, being indicated as a crucial aspect in all metastatic steps. To investigate the complex interrelation between the tumor and the microenvironment, both in vitro and in vivo models have been exploited. In vitro models have some advantages over in vivo, mainly the possibility to thoroughly dissect in controlled conditions and with only human cells the cellular and molecular mechanisms underlying the metastatic progression. In this article we will review the main results deriving from in vitro co-culture models, describing mechanisms activated in the crosstalk between breast cancer and bone cells which drive the different metastatic steps.     

Extending multimedia languages to support multimodal user interactions

Historically, the Multimedia community research has focused on output modalities, through studies on timing and multimedia processing. The Multimodal Interaction community, on the other hand, has focused on user-generated modalities, through studies on Multimodal User Interfaces (MUI). In this paper, aiming to assist the development of multimedia applications with MUIs, we propose the integration of concepts from those two communities in a unique high-level programming framework. The framework integrates user modalities —both user-generated (e.g., speech, gestures) and user-consumed (e.g., audiovisual, haptic)— in declarative programming languages for the specification of interactive multimedia applications. To illustrate our approach, we instantiate the framework in the NCL (Nested Context Language) multimedia language. NCL is the declarative language for developing interactive applications for Brazilian Digital TV and an ITU-T Recommendation for IPTV services. To help evaluate our approach, we discuss a usage scenario and implement it as an NCL application extended with the proposed multimodal features. Also, we compare the expressiveness of the multimodal NCL against existing multimedia and multimodal languages, for both input and output modalities.

     

Investigation of smoke gases and temperatures during car fire – large‐scale and small‐scale tests and numerical investigations

The hazards for passengers during vehicle fires result from the increasing temperature and the emitted smoke gases. A fire was set on a car to investigate the development of temperature and of gaseous fire products in the passenger compartment. The study was based on a full‐scale test with a reconstructed scene of a serious car fire. The aim of this work was to identify the conditions for self‐rescuing of passengers during a car fire. A dummy, equipped with several thermocouples, was placed on the driver's seat. Also, the smoke gases were continuously collected through a removable probe sensor corresponding to the nose of the dummy in the passenger compartment and analyzed using Fourier transform infrared spectroscopy. Additionally, several car components were investigated in the smoke density chamber (smoke emission and smoke gas composition). It was found that the toxic gases already reached hazardous levels by 5 min, while the temperatures at the dummy were at that time less than 80 °C. The toxicity of smoke gases was assessed using the fractional effective dose concept. The various experimentally parameters (temperature and smoke gas composition) were implemented into numerical simulations with fire dynamics simulator. Both the experimental data and the numerical simulations are presented and discussed. Copyright © 2015 John Wiley & Sons, Ltd.     

Coarse-grained and All-atom Simulations towards the Early and Late Steps of Amyloid Fibril Formation

Alzheimer's disease is the most common neurodegenerative disease. Experiments and computer simulations can complement one another to provide a full and in-depth understanding of many aspects in the amyloid field at the atomistic level. Here, we review results of our coarse-grained and all-atom simulations in aqueous solution aimed at determining: 1) early aggregation steps of short linear peptides; 2) nucleation size number; 3) solution structure of the Aβ_1–40/Aβ_1–42 wild-type dimers; 4) impact of FAD (familial forms of Alzheimer's disease) mutations on the structure of Aβ_1–40/Aβ_1–42 dimers; and 5) impact of protective mutations on the structure of Aβ_1–40/Aβ_1–42 dimers.     

Microflora associated with primary endodontic infections: Correlations among sem evaluation,clinical features,and radiographic findings

The aim of the present study was to characterize, by means of SEM, primary endodontic infections and to correlate with clinical and radiographic findings. Twelve (12) human extracted teeth (19 roots) presenting primary endodontic infection were examined. SEM qualitative observations of bacterial and defense cells, their features and distribution within the root canal lumen and root dentine were recorded for association with clinical and radiographic tabled data. Although a direct correlation between biofilm composition and clinical/radiographic findings was not established, structural organization and distribution of the biofilm, as well as the characteristics of host response, could be easily related to those features. Bacterial biofilm was predominant at the apical third. Symptomatic apical periodontitis was related to presence of bacterial biofilm all thirds. Defense cells could be seen in the apical third of some samples. These cells were present in all thirds in some of the cases with open cavities. The correlations performed in this study allowed a better understanding of the picture of primary endodontic infection, host response and relevant clinical features. The combined use of scanning electron microscopy with clinical and radiographic evaluation has the potential to overcome some limits of the current knowledge related to pulpal and periapical diseases, providing important insights for improving treatment strategies. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.     

Effects of parboiling,storage and cooking on the levels of tocopherols,tocotrienols and γ-oryzanol in brown rice (Oryza sativa L.)

Vitamin E and γ-oryzanol display a wide range of biological activities including hypocholesterolemic, anti-inflammatory and antioxidant activities. Although white rice is far more popular worldwide, consumption of brown rice is increasing, partially on account of the presence of bioactive compounds; however, the effects of parboiling, storage and cooking on them are not well-characterized. The effects of parboiling and a 6-month storage period on the contents of vitamin E and γ-oryzanol in three brown rice cultivars grown in three different locations in Brazil were investigated. Also, their levels in branded non-parboiled and parboiled brown rice were monitored before and after cooking. Vitamin E homologues and γ-oryzanol were separated by RP-HPLC equipped with PDA and fluorescence detectors. The average levels of total tocols and γ-oryzanol in the raw brown rice cultivars studied were 25 and 188 mg/kg, respectively. Of the vitamin E homologues, γ-tocotrienol contributed with 74% of total tocols, followed by α-tocopherol, α-tocotrienol and γ-tocopherol in minor quantities. The combined processes, parboiling, storage and cooking, led to an approximate 90% reduction in tocols and only γ-tocotrienol was detectable after any of the processes. Parboiling followed by storage resulted in an approximate 40% loss of γ-oryzanol. Cooking had almost no further effect over γ-oryzanol levels in parboiled rice previously stored for 6 months.