Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis

As a result of deprivation of oxygen (hypoxia) and nutrients, the growth and viability of cells is reduced. Hypoxia-inducible factor (HIF)-1alpha helps to restore oxygen homeostasis by inducing glycolysis, erythropoiesis and angiogenesis. Here we show that hypoxia and hypoglycaemia reduce proliferation and increase apoptosis in wild-type (HIF-1alpha+/+) embryonic stem (ES) cells, but not in ES cells with inactivated HIF-1alpha genes (HIF-1alpha-/-); however, a deficiency of HIF-1alpha does not affect apoptosis induced by cytokines. We find that hypoxia/hypoglycaemia-regulated genes involved in controlling the cell cycle are either HIF-1alpha-dependent (those encoding the proteins p53, p21, Bcl-2) or HIF-1alpha-independent (p27, GADD153), suggesting that there are at least two different adaptive responses to being deprived of oxygen and nutrients. Loss of HIF-1alpha reduces hypoxia-induced expression of vascular endothelial growth factor, prevents formation of large vessels in ES-derived tumours, and impairs vascular function, resulting in hypoxic microenvironments within the tumour mass. However, growth of HIF-1alpha tumours was not retarded but was accelerated, owing to decreased hypoxia-induced apoptosis and increased stress-induced proliferation. As hypoxic stress contributes to many (patho)biological disorders, this new role for HIF-1alpha in hypoxic control of cell growth and death may be of general pathophysiological importance.     

The normal spectral emittance of erbium,dysprosium and samarium above 1000 K

An apparatus for measuring the normal spectral emittance (λ = 0.645 μ.) of metals with very high vapor pressures has been designed and used for erbium, dysprosium and samarium at temperatures above 1000 K. The samples are maintained in an argon atmosphere at a pressure slightly above atmospheric pressure. The measured normal spectral emittances are: Erbium, (solid), ∈ _nλ = 0.372 ± 0.011; (liquid), ∈ _nλ = 0.372 ± 0.011; Dysprosium, (solid), ∈ _nλ = 0.631 – 1.889 × 10^–4 T (deg K); (liquid), ∈ _nλ = 0.297 ± 0.088; Samarium, (solid), ∈ _nλ = 0.437 ± 0.030; (liquid, estimated), ∈ _nλ = 0.437 ± 0.03. TERRY S. KING, formerly Undergraduate Research Helper. DORAB N. BARIA, formerly NSF Presidential Intern and Post-Doctoral Fellow. Prepared for the Energy Research and Development Administration under Contract No. W-7405-eng-82.     

Effect of surface conditions on the normal spectral emittance of titanium and zirconium between 1000 and 1800 K

The normal spectral emittance of Ti and Zr was obtained in the temperature range 1100 to 1800 K and wavelength 0.645 μ for electrochemically polished, mechanically polished and 60 grit ground surfaces at a pressure below 10^-8 mm Hg. The emittance at the melting point of both metals was obtained by extrapolation of an empirical equation fitted to the experimental data obtained for the electrochemically polished surface and was found to be in good agreement with reported values. The considerable grain growth on the surface affected the emittance. The emittance was found to increase with temperature up to 1300 K and then decreased with further increase in temperature. Formarly a National Science Foundation Presidential Intern and an Ames Laboratory-USAEC Postdoctoral Fellow, Iowa State University, Ames, Iowa.     

Wireless LANs: From WarChalking to Open Access Networks

This work discusses the evolution of W-LANs from their current status of wireless termination of LAN services to a possible global infrastructure where the access networks become open to multiple operators and a vehicle of a win-win scenario, where both users and operators benefit from the new network architecture. The idea of Open Access Networks (OANs) can go beyond wireless HotSpots and be generalized to a generic shared access infrastructure that fosters service operators competition and drastically reduces the cost of last mile coverage.The general concept of Open Access Networks is detailed, highlighting its difference with the more traditional model of vertical integration of the access network into the global service. About the OANs development, it is shown how to support the quick and smooth evolution of the infrastructure toward a widespread and reliable communication support.Business models are discussed by mentioning the different actors, the market organization and the different organization forms.The final part of the paper is devoted to technical challenges such as access control, security, privacy, roaming, resource exploitation and service differentiation. As an example of how to tackle these problems, we discuss a pricing technique devoted to resource management and billing support.In addition we present a simulation on how the OAN concept can speed-up the deployment of broadband access in a real case.A preliminary version of this paper with the title “Global Growth of Open Access Networks: from WarChalking and Connection Sharing to Sustainable Business” was presented at WMASH 2003. Roberto Battiti received the Laurea degree from the University of Trento, Italy, in 1985 and the Ph.D. degree from the California Institute of Technology (Caltech), USA, in 1990. He has been a consultant in the area of parallel computing and pattern recognition and since 1991 he has been a faculty member at the University of Trento, where he is now full professor of Computer Networks. His main research interests are heuristic algorithms for optimization problems, in particular reactive search algorithms for maximum clique, maximum satisfiability, graph coloring, networks and massively parallel architectures, code assignment in wireless and cellular networks, protocols for pricing and Quality of Service in wireless networks.Prof. R. Battiti is currently Dean of the international Graduate School in Information and Communication Technologies at Trento, Deputy Dean at the Faculty of Science, member of the advisory committee for the future Telecommunications Plan of the Autonomous Province of Trento. Prof. R. Battiti is associate editor of various scientific journals. He is the author of more than fifty scientific publications, including special issues dedicated to experimental algorithmics and wireless on-demand networks.R. Battiti is a member of IEEE Computer Society and ACM Sigmobile. Renato Lo Cigno is Associate Professor at the Department of Computer Science and Telecommunications (DIT) of the University of Trento, Italy. He received a Dr. Ing. degree in Electronic Engineering from Politecnico di Torino in 1988. From 1989 to 2002 has been with the Telecommunication Research Group of the Electronics Department of Politecnico di Torino.From June 1998 to February 1999, he was at the CS Department at UCLA as Visiting Scholar under grant CNR 203.15.8. He is coauthor of more than 90 journal and conference papers in the area of communication networks and systems. His curent research interests are in performance evaluation of wired and wireless networks, modeling and simulation techniques, flow and congestion control, as well as network management and architecture. Renato Lo Cigno is member of the IEEE Communication Society and ACM Sigcomm. Mikalai Sabel received his master degree in radio physics from Belorussian State University in 2002. He is now pursuing his Ph.D. at the international Graduate School of Information and Communication Technologies at University of Trento, Italy. His research interest include wireless LANs, pricing and incentive engineering, IEEE802.11 MAC protocol. His current research is focused on optimization algorithms that can provide stability and maximum global welfare in a non-cooperative environment where singles tries to maximize their own benefit function. Experimentation of such schemes to pricing techniques and micro-payments in TLC networks provides the application environment of the fundamental research. Fredrik Orava is associate professor at KTH, Stockholm, Sweden. He conducts research into scalable (in terms of cost, capacity, number of users and devices etc.) communication system architectures and technologies. He has a MSc in engineering physics from Uppsala University and a PhD in computer systems also from Uppsala University. He previously held positions as researcher at the Swedish Institute for Computer Science (SICS); senior lecturer and manager of the telecommunications systems laboratory at the department for teleinformatics, KTH; acting professor in telecomunication systems at KTH; director for the Swedish Centre for Internet Technologies; and vice president of Dynarc AB with world wide responsibility for product management. Dynarc AB develops, manufactures and sells IP routers for optical IP networks. Bjorn Pehrson is a professor of telecommunication systems at KTH, the Royal Institute of Technology in Stockholm, since 1992, where he has also served as a department chairman and vice dean. He had his PhD from Uppsala University 1975 where he also served as senior lecturer and established a research group in computer and communication technology. During the period 1985-1992 he participated in the establishment of the Swedish Institute of Computer Science. Björn Pehrson research interests are currently focussed on open network topologies allowing different actors forming new value chains to build networks together based on different, sometimes very local business models. The results are applied in testbeds, especially in rural areas and in developing countries.This revised version was published online in August 2005 with a corrected cover date.     

Phenolic compounds in Imburana (<Emphasis Type="Italic">Amburana cearensis)</Emphasis> powder extracts

In response to the increasing demand for aged distilled spirits, wood extracts have been investigated as accelerating agents in distilled spirits. The aim of this work was to obtain and to evaluate the potential use of Amburana cearensis powder extracts as an accelerating aging agent in sugar cane distilled spirits. Wood powder was submitted to different toasting treatments using conventional drying oven and microwave oven. Instead of using the traditional reflux extraction method, the phenolic compounds were extracted by means of ultrasonic treatment using a 50% ethanolic solution with pH adjusted to 4.5. The concentration of gallic acid, 5-hydroxymethylfurfural, furfural, vanillic acid, syringic acid, vanillin, coniferaldehyde, sinapaldehyde and coumarin, determined by high performance liquid chromatography (HPLC) were used as quality parameters of powder toasting treatment and ethanolic extraction. The use of toasted wood powder combined with ultrasonic extraction showed higher yields when compared to the traditional solvent extraction method when obtaining wood extracts. The extraction method presented in this work is also less time consuming.     

On exact selection of minimally unsatisfiable subformulae

A minimally unsatisfiable subformula (MUS) is a subset of clauses of a given CNF formula which is unsatisfiable but becomes satisfiable as soon as any of its clauses is removed. The selection of a MUS is of great relevance in many practical applications. This expecially holds when the propositional formula encoding the application is required to have a well-defined satisfiability property (either to be satisfiable or to be unsatisfiable). While selection of a MUS is a hard problem in general, we show classes of formulae where this problem can be solved efficiently. This is done by using a variant of Farkas lemma and solving a linear programming problem. Successful results on real-world contradiction detection problems are presented.     

Quantitative GC-ECD Analysis of Halogenated Metabolites: Determination of Surface and Within-Thallus Elatol of Laurencia obtusa

Information on natural concentrations or variability of secondary metabolites in marine organisms may be important both to ecological/evolutionary and applied approaches. A gas chromatographic procedure with an electron capture detector (GC-ECD) was developed to quantify the sesquiterpenoid elatol at the surface and within-thalli of 70 specimens of the red seaweed Laurencia obtusa. The concentration of elatol was highest within-thalli [9.89 mg g⁻¹ of L. obtusa, dry weight (d.w.)], compared to lower values found at the surface [0.006 mg g⁻¹ of L.obtusa (d.w.), or 0.5–10.0 ng cm⁻²]. This method provides a rapid and inexpensive quantification of small quantities of elatol, and probably may also be used to quantify other halogenated compounds usually found in red seaweeds.     

Predicting odour emissions from wastewater treatment plants by means of odour emission factors

In this study, the results of odour concentration measurements on different wastewater treatment plants are presented and used in order to estimate the odour emission factors relevant to single odour sources. An odour emission factor is a representative value that relates the quantity of odour released to the atmosphere to a specific activity index, which in this case was the plant treatment capacity, resulting in an odour emission factor expressed in odour units per cubic metre of treated sewage. The results show that the major odour source of a wastewater treatment plant is represented by the primary sedimentation (with an OEF equal to 1.9 × 10⁵ ou_E m⁻³). In general, the highest OEFs are observed in correspondence of the first steps of the wastewater depuration cycle (OEF between 1.1 × 10⁴ ou_E m⁻³ and 1.9 × 10⁵ ou_E m⁻³) and tend to decrease along the depuration process (OEF between 7.4 × 10³ ou_E m⁻³ and 4.3 × 10⁴ ou_E m⁻³). In general, the OEFs calculated according to this approach represent a model for a rough prediction of odour emissions independently from the specific characteristics of the different plants.     

Storage of green coffee in hermetic packaging injected with CO2

The objective of this study was to evaluate the physical, chemical, and sensory qualities of green coffee beans (Coffea arabica L.) during storage in different types of packaging. Coffee was stored from October 2008 to September 2009 in a warehouse of the Agriculture Society Ltda. (SAAG) in Santana da Vargem, southern Minas Gerais State, Brazil. The treatments in the factorial design consisted of two types of packaging (hermetic big bags with the injection of up to 60% CO₂ in a controlled atmosphere; similar bags but without the injection of CO₂ in a modified atmosphere) and three sampling positions in the bags (high, medium, and low). At 3-month intervals during a 12-month period, grains were analyzed to determine their water content, color, electrical conductivity, potassium lixiviation, and content of sugars. Sensory analysis was also conducted at these sampling times. The storage of green coffee beans in hermetic big bags on a commercial scale under modified and controlled atmospheric conditions is viable over a 12-month period. The coffee packed in big bags maintained its quality and exhibited an intensification of the green coloration of the grains during storage. Sensory analysis of coffee beans stored in a controlled atmosphere showed that the medium sampling position yielded the best ratings. The results of this analysis demonstrated that this storage technique can potentially increase the effectiveness of methods used to preserve the sensory quality of coffee beans.