Bubbles in solvent microextraction: the influence of intentionally introduced bubbles on extraction efficiency

Significant improvements to microdrop extractions of triazine pesticides are realized by the intentional incorporation of an air bubble into the solvent microdroplet used in this microextraction technique. The increase is attributed partly to greater droplet surface area resulting from the air bubble being incorporated into the solvent droplet as opposed to it sitting thereon and partly to thin film phenomena. The method is useful at nanogram/liter levels (LOD 0.002-0.012 μg/L, LOQ 0.007-0.039 μg/L), is precise (7-12% at 10 μg/L concentration level), and is validated against certified reference materials containing 0.5 and 5.0 μg/L analyte. It tolerates water and fruit juice as matrixes without serious matrix effects. This new development brings a simple, inexpensive, and efficient preconcentration technique to bear which rivals solid phase microextraction methods.     

C60 secondary ion Fourier transform ion cyclotron resonance mass spectrometry

Secondary ion mass spectrometry (SIMS) has seen increased application for high spatial resolution chemical imaging of complex biological surfaces. The advent and commercial availability of cluster and polyatomic primary ion sources (e.g., Au and Bi cluster and buckminsterfullerene (C(60))) provide improved secondary ion yield and decreased fragmentation of surface species, thus improving accessibility of intact molecular ions for SIMS analysis. However, full exploitation of the advantages of these new primary ion sources has been limited, due to the use of low mass resolution mass spectrometers without tandem MS to enable enhanced structural identification capabilities. Similarly, high mass resolution and high mass measurement accuracy would greatly improve the chemical specificity of SIMS. Here we combine, for the first time, the advantages of a C(60) primary ion source with the ultrahigh mass resolving power and high mass measurement accuracy of Fourier transform ion cyclotron resonance mass spectrometry. Mass resolving power in excess of 100?000 (m/Δm(50%)) is demonstrated, with a root-mean-square mass measurement accuracy below 1 part-per-million. Imaging of mouse brain tissue at 40 μm pixel size is shown. Tandem mass spectrometry of ions from biological tissue is demonstrated and molecular formulas were assigned for fragment ion identification.     

Channelized Hotelling observers for the assessment of volumetric imaging data sets

Current clinical practice is rapidly moving in the direction of volumetric imaging. For two-dimensional (2D) images, task-based medical image quality is often assessed using numerical model observers. For three-dimensional (3D) images, however, these models have been little explored so far. In this work, first, two novel designs of a multislice channelized Hotelling observer (CHO) are proposed for the task of detecting 3D signals in 3D images. The novel designs are then compared and evaluated in a simulation study with five different CHO designs: a single-slice model, three multislice models, and a volumetric model. Four different random background statistics are considered, both gaussian (noncorrelated and correlated gaussian noise) and non-gaussian (lumpy and clustered lumpy backgrounds). Overall, the results show that the volumetric model outperforms the others, while the disparity between the models decreases for greater complexity of the detection task. Among the multislice models, the second proposed CHO could most closely approach the volumetric model, whereas the first new CHO seems to be least affected by the number of training samples.     

Physicochemical,carbohydrate and sensory characteristics of highbush and rabbiteye blueberry cultivars

The chemical composition, colour and texture of fresh berries from three rabbiteye (‘Climax’, ‘Premier’ and ‘Tifblue’) and two highbush (‘Bluecrop’ and ‘Jersey’) blueberry cultivars were measured. Shear (482 vs 290 N), compression (6.50 vs 3.96 N) and puncture forces (1.48 and 0.85 vs 0.81 and 0.43 N) were higher (P ≤ 0.05) for rabbiteye than for highbush cultivars. Shear for ‘Bluecrop’ blueberries averaged 254 N, whereas for ‘Climax’ it was 565 N. The puncture force required to penetrate the skin was lower for ‘Bluecrop’ (0.78 N) and ‘Jersey’ (0.83 N) and higher for ‘Climax’ (1.71 N). However, sensory panellists found no differences (P > 0.05) in colour, flavour or skin toughness among the five cultivars. ‘Climax’ was rated lower in seediness. Rabbiteye cultivars contained more fibre and complex polysaccharides than highbush cultivars. These differences in complex carbohydrates that make up the cell wall layers may contribute to the toughness of rabbiteye cultivars and their possible longer fresh shelf‐life, though taste panellists did not find this toughness objectionable. Copyright © 2005 Society of Chemical Industry     

Polynuclear aromatic hydrocarbons (PAHs) mediate cadmium toxicity to an emergent wetland species

Growth and pollutant removal by emergent wetland plants may be influenced by interactions among mixed pollutants in constructed wetlands. A glasshouse experiment was conducted to investigate interactive effects of cadmium (Cd) × polynuclear aromatic hydrocarbons (PAHs) × plant treatments on growth of Juncus subsecundus, Cd and PAH removal from soil and the total number of microorganisms in soil. Growth and biomass of J. subsecundus were significantly influenced by interaction of Cd and PAHs, significantly decreasing with either Cd or PAH additions, but with the effect of Cd on plant growth being stronger than that of PAHs. The mixture of low Cd and low PAH lessened Cd toxicity to plants, resulting in improved plant growth and increased Cd accumulation in plant tissues, thus enhancing Cd removal by plants. The dissipation of PAHs in soils was significantly influenced by interactions of Cd, PAH and plant presence or absence. The total number of microorganisms in soils was significantly increased by the PAH additions. The interactive effect of Cd and PAHs on plant growth may be linked to the changes in the abundance of microorganisms in the rhizosphere, probably via a positive effect of PAH metabolites and/or phytohormones produced by microorganisms on plant growth.     

Pumpkin oil cake protein as a new carrier for encapsulation incorporated in food matrix: Effect of processing,storage and in vitro digestion on bioactivity

In this paper, valorisation of pumpkin oil cake as source of new protein carrier material for encapsulation was examined in order to enrich real food products. Cookies were used as a model system for addition of encapsulates at three different levels: 10%, 15% and 20%. The physicochemical and sensory properties were investigated, as well as conditions of stability after storage, in terms of total phenolic and betalain content. Furthermore, antioxidant, α-glucosidase and ACE inhibitory potential of cookies was evaluated after simulated gastrointestinal digestion. The cookies showed good technological quality as well as acceptable sensorial properties. The addition of encapsulate increases the total phenolic and betalain contents and shoved significant stability during storage time. Novel peptides were produced after gastrointestinal digestion, which enhanced bioactive properties of enriched products through synergistic action with active compounds from beetroot juice. Obtained digest shoved significant antioxidant, antidiabetic and antihypertension activities, hence ensuring a potential healing effect that could be reached by consuming these cookies.     

Influence of winemaking techniques on the resveratrol content, total phenolic content and antioxidant potential of red wines

In this paper, the influence of winemaking techniques and cultivars on the resveratrol content, total phenolic content and antioxidant potential of red wines was studied. Wines were made from the cultivars Merlot, Cabernet Sauvignon, Pinot Noir and Prokupac. Applied winemaking technologies included thermovinification and separation of must from pomace. The analysis of trans- and cis-resveratrol in wines was performed by HPLC, while the total phenolic content and antioxidant potential was determined by spectrophotometric methods. The total resveratrol content in analysed samples ranged from 0.35 to 4.85 mg/l; Merlot wines had the highest average resveratrol content, while the lowest was found for native cultivar Prokupac. Although the resveratrol content depended on grape variety, correlation between the winemaking technology applied and the resveratrol level in wines was not observed. The total phenolic content (TPC) varied from 544.4 to 1410.4 mg/l expressed as gallic acid equivalents, and the antioxidant potential, assayed by DPPH in terms of SC₅₀ (mean scavenging concentration), ranged from 0.58 to 2.91 μl/ml. Obtained results showed that thermovinificated wine samples had higher amount of phenolic compounds. Significant negative correlation was observed (p < 0.05) between the total phenolic content and SC₅₀, but there was no significant correlation (p > 0.05) between the resveratrol content and SC₅₀ or TPC. This study could contribute to the establishment of optimal conditions for producing red wines which contain more beneficial phenolic compounds.     

On the k-th root in circular arithmetic

The representation of thek-th root of a complex circular intervalZ={c;r} is considered in this paper. Thek-th root is defined by the circular intervals which include the exact regionZ ^1/k={z:z ^k ∈Z}. Two representations are given: (i) the centered inclusive disks \( \cup \{ c^{{1 \mathord{\left/ {\vphantom {1 k}} \right. \kern-0em} k}} ; \mathop {\max }\limits_{z \in Z} |z^{{1 \mathord{\left/ {\vphantom {1 k}} \right. \kern-0em} k}} - c^{{1 \mathord{\left/ {\vphantom {1 k}} \right. \kern-0em} k}} |\} \) and (ii) the diametrical inclusive disks with the diameter which is equal to the diameter of the regionZ ^1/k.