White cabbage fermentation improves ascorbigen content,antioxidant and nitric oxide production inhibitory activity in LPS-induced macrophages

Consumption of foods rich in dietary antioxidants and anti-inflammatory compounds is becoming a key strategy to lower oxidative stress and inflammation. The objective of this work was to study the effect of fermentation and starter culture on ascorbigen (ABG) and vitamin C content, as well as antioxidant and anti-inflammatory properties of white cabbage (Brassica oleracea var. capitata cv. Megaton). Lactobacillus plantarum CECT 748 (LP), Leuconostoc mesenteroides CECT 219 (LM) or a mixed culture of both strains at 1:1 ratio (LPM) were used as starter cultures in sauerkraut manufacture. Microbiological and sensorial quality of sauerkraut was also examined. White cabbage fermentation increased (P < 0.05) ABG content (up to 12-fold), oxygen radical absorbance capacity (ORAC) values (up to 2-fold) and NO production inhibitory potency (up to 2.6-fold). Vitamin C content slightly decreased (P < 0.05) up to 1.4-fold during fermentation. LM sauerkraut showed the highest (P < 0.05) ABG concentration (204.8 μmol/100 g d.w.), ORAC values (164.0 μmol Trolox/g d.w.) and NO inhibitory potency (IC₅₀ = 60.8 μg extract/mL). The microbiological quality of LM, LP and LPM sauerkrauts was satisfactory. Experimental sauerkrauts showed higher overall acceptability (P < 0.05) compared to commercial products. Consequently, selection of starter culture is of great importance in the manufacture of sauerkraut with improved content of bioactive compounds and health-promoting potential.     

Antioxidant mechanism of grape procyanidins in muscle tissues: Redox interactions with endogenous ascorbic acid and α-tocopherol

The present study investigates the antioxidant mechanism of grape procyanidins and, in particular, their aptitude to establish redox interactions with two important components of the endogenous antioxidant system of muscle tissues, α-tocopherol (α-TOH) and ascorbic acid (AA). To this end, the progress of lipid oxidation was monitored in fish muscle supplemented with grape procyanidins at the concentrations usually employed in antioxidant food applications, and then related to the redox stability of the endogenous α-TOH and AA. In addition to the lipid oxidation protective effect, the incorporation of procyanidins also provided an improvement of the redox stability of the endogenous components in a straight procyanidinic concentration-dependent manner. Results showed the capacity of procyanidins to repair oxidised α-TOH at medium-long term, and to delay the AA depletion. Therefore, such cooperative redox interaction of exogenous procyanidins adequately complements the natural α-TOH regenerative system supplied by AA that is efficient at the early post mortem stages.     

Segmentation of neuronal nuclei based on clump splitting and a two-step binarization of images

In this paper we present an algorithm to segment the nuclei of neuronal cells in confocal microscopy images, a key technical problem in many experimental studies in the field of neuroscience. We describe the whole procedure, from the original images to the segmented individual nuclei, paying particular attention to the binarization of the images, which is not straightforward due to the technical difficulties related to the visualization of nuclei as individual objects and incomplete and irregular staining. We have focused on the division of clusters of nuclei that appear frequently in these images. Thus we have developed a clump-splitting algorithm to separate touching or overlapping nuclei allowing us to accurate account for both the number and size of the nuclei. The results presented in the paper show that the proposed algorithm performs well on different sets of images from different layers of the cerebral cortex.     

Analyzing locality over a P2P computing architecture

A characteristic of Peer-to-Peer (P2P) computing networks is their huge number of different computational resources scattered across the Internet. Gathering peers into markets according to their multi-attribute computational resources makes it easier to manage these environments. This solution is known as market overlay. In this context, the closeness of the markets with similar resources, known as locality, is a key feature for ensuring good P2P resource management. Thus, the locality feature over a market overlay allows a lack of resources in a given market to be compensated quickly by any other market with similar resources, whenever these are close to each other. Consequently, locality becomes an essential challenge.This paper addresses the analysis of the locality of P2P market over-lays. According to this, a new procedure for measuring locality is applied together with an extensive analysis of some well-known structured P2P overlays. Based on this analysis, a new P2P computing architecture, named DisCoP, oriented towards optimizing locality is proposed. Our proposal gathers the peers into markets according to their computational resources. A Hilbert function is used to arrange multi-attribute markets in an ordered and mono-dimensional space and the markets are linked by means of a Bruijn graph. In order to maintain the DisCoP locality whenever the overlay is not completed, a solution based on the virtualization of markets is also proposed. Finally, the DisCoP locality is tested together with the proposed virtualization method for approximate searches over uncompleted overlays. The simulation results show that approximate searches exploit the DisCoP locality efficiently.     

Effect of moisture on salmonella spp. heat resistance in cocoa and hazelnut shells

The heat resistance of food-poisoning outbreak and non-outbreak associated strains of Salmonella (S. Enteritidis, S. Montevideo, S. Napoli, S. Oranienburg, S. Poona, S. Senftenberg and S. Typhimurium) was established in confectionery-related materials such as crushed cocoa bean and hazelnut shells at low moisture contents (≤ 4% w/w). The two most heat resistant strains in cocoa and hazelnut shells at ca. 4% w/w moisture were S. Oranienburg and S. Enteritidis PT30. Both strains were associated with outbreaks from dried materials. Their D_100°C values were ca. 2.5 min in crushed cocoa bean shells and 7–11 min in crushed hazelnut shells. Addition of moisture to ca. 7% w/w markedly reduced D-values (D_80°C of 2–4.5 min) for both strains in the two matrices.     

Electrical and mechanical characterization by instrumented indentation technique of La0.85Sr0.15Ga0.8Mg0.2O3−δ electrolyte for SOFCs

La_0.85Sr_0.15Ga_0.8Mg_0.2O_3?δ pellets obtained by the polymeric organic complex solution method, isostatic pressing and sintering at 1350 °C have been electrical and mechanically studied. Electrical measurements evidenced reasonable ionic conductivities (0.01 S cm^?1 at 800 °C), which were comparable to those reported for the La_1?xSr_xGa_1?yMg_yO_3?δ prepared by other synthesis methods. On the other hand, the mechanical properties (elastic modulus, E and hardness, H) have been determined at micro/nanometric scale using the instrumented indentation technique. While E did not vary significantly with the increasing indentation depth (h), H values strongly decreased with the indentation depth up to 500 nm. For h > 500 nm, both mechanical properties remained almost constant, thus obtaining E = 271 ± 6 GPa and H = 13.2 ± 0.4 GPa. Finally, the residual imprints and fracture mechanisms have been observed by atomic force microscopy (AFM).     

On the Use of Static Temperature Measurements as Process Variation Observable

In this paper we present the use of static temperature measurements as process variation observable. Contrary to previously published thermal testing methods, the proposed methodology does not need an excitation signal, thus reducing test cost and improving built-in capabilities of thermal monitoring. The feasibility of the technique and a complete test methodology is presented using a narrowband LNA as example. Finally, a complete electro-thermal co-simulation test bench between the LNA and a differential temperature sensor embedded in the same silicon die is presented in order to validate the results. Results prove that RF figures of merit can be extracted from DC temperature measurements done without loading or exciting the RF circuit under test.     

Optimising by the response surface methodology the enzymatic elimination of clogging of a microfiltration membrane by pectin cake

The aim of this paper, in which clogging occurs on the filter at a constant vacuum pressure and constant pectin weight, is to find the optimal values of the variables, temperature and time, to know the best conditions to reduce this clogging by enzymatic hydrolysis. Moreover, different properties of the compression of the cake in the filter were determined such as the filtering medium total resistance (R_fT), bed porosity (ε) and the cake‐specific resistance (α) at different times and temperatures. The filter area was 0.142 m², and the value of the mass fraction of solids in the filtered suspension (S) was 0.12. Scanning electron microsopy (SEM) analyses were performed. The results showed that the compressive stress corresponding to the cake improves the flow rate in the filtration flux at the optimal temperature and time for the process, where the enzyme reaches its highest activity. The test results of this study can be applied in different system of filters during cross‐flow filtration.     

Analysis of triclosan and 4n‐nonylphenol in Colombian reservoir water by gas chromatography‐mass spectrometry

In this work were determined triclosan and 4n‐nonylphenol in water from a reservoir that is used to provide water to a purification plant in an important city in Colombia. The analytical methodology was validated using solid‐phase extraction and analysis by gas chromatography‐mass spectrometry (GC‐MS). The analysis by GC‐MS showed good linearity in the range of 0.05–5 μg/L for both compounds. Recoveries from 79 to 109% and standard deviations of 2.5–7.7 for low concentrations and from 3.8 to 9.6 (n = 5) for high concentrations were obtained for both compounds. In Colombia, this is the first time that these compounds have been analysed in water supplying of a drinking water treatment plant. The validated method was applied to the analysis of 27 samples collected in August 2010 in 11 locations from the reservoir and in the influent and effluent of the drinking water treatment plant. In total, seven samples were found to contain triclosan.