A Comparison of State-of-the-Art Classification Techniques with Application to Cytogenetics

Several state-of-the-art techniques – a neural network, Bayesian neural network, support vector machine and naive Bayesian classifier – are experimentally evaluated in discriminating fluorescence   in situ hybridisation (FISH) signals. Highly-accurate classification of valid signals and artifacts of several cytogenetic probes (colours) is required for detecting abnormalities in FISH images. More than 3100 FISH signals are classified by each of the techniques into colour and as real or artifact with accuracies of around 98% and 88%, respectively. The results of the comparison also show a trade-off between simplicity represented by the naive Bayesian classifier, and high classification performance represented by the other techniques.     

An opportunity cost approach for job assignment in a scalablecomputing cluster

A new method is presented for job assignment to and reassignment between machines in a computing cluster. Our method is based on a theoretical framework that has been experimentally tested and shown to be useful in practice. This “opportunity cost” method converts the usage of several heterogeneous resources in a machine to a single homogeneous “cost.” Assignment and reassignment are then performed based on that cost. This is in contrast to traditional, ad hoc methods for job assignment and reassignment. These treated each resource as an independent entity with its own constraints, as there was no clean way to balance one resource against another. Our method has been tested by simulations, as well as real executions, and was found to perform well     

The value of precise preoperative localization of colonic arteriovenous malformation in childhood

Massive hemorrhage from the gastrointestinal tract in a 12-yr-old boy caused by a congenital atypically located colonic arteriovenous malformation is described. Guided and "clean" resection of the involved colon was possible due to preoperative selective angiography, which proved to be the most efficient diagnostic tool. Histologic documentation of this rare pathology in childhood is presented, and the classification and features of the disease are briefly reviewed.     

Combatting sexual harassment.

Comments that S. Riger's (see record 1991-27670-001) overemphasis on presumed gender-biased definitions and on changing legislation and organizational policy related to sexual harassment (SH) detracts from her aim of promoting the growth and development of women in a changing society. Interventions are needed for modifying the social atmosphere that encourages SH and the educational procedures designed to help women recognize and combat SH. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Emotional support and suicide prevention through the Internet: A field project report

The Internet can be efficiently exploited to help people in severe emotional distress, including those contemplating suicide. Based on this premise and guided by various psychological principles that characterize Internet communication and behavior, on the one hand, and by factors related to the provision of emotional support, on the other, an Israeli project with the Hebrew acronym SAHAR was established. The idea behind SAHAR was to initiate an anonymous, confidential online environment that would attract people in a crisis situation and offer them a listening ear, mental support, and warmth, provided by anonymous, skilled helpers. At the heart of this exclusively online service is a content-rich Hebrew website (http://www.sahar.org.il) that provides relevant and continuously updated information for people in need. Accessed through the site, SAHAR offers, in addition, personal communication to users through synchronous and asynchronous support. For group communication, SAHAR provides online forums (a chat room will soon be launched). The website is accessed more than 10,000 times a month, or 350 times a day, a considerable number relative to Israel’s small population. Approximately 1000 personal contacts with SAHAR itself take place, each month, of which at least a third of the distressed users are clearly suicidal. The forums receive over 200 new messages a day. SAHAR on numerous occasions has participated in rescue operations of individuals who threatened to commit suicide or were actually in the process of trying. In many other cases, a supportive conversation or a referral to appropriate help resources prevented hasty decisions by highly distressed, desperate people contemplating suicide. Feedback by users also indicates the success of SAHAR as a unique psychological application on the Internet.     

Segmentation and classification of dot and non-dot-like fluorescence in situ hybridization signals for automated detection of cytogenetic abnormalities.

Signal segmentation and classification of fluorescence in situ hybridization (FISH) images are essential for the detection of cytogenetic abnormalities. Since current methods are limited to dot-like signal analysis, we propose a methodology for segmentation and classification of dot and non-dot-like signals. First, nuclei are segmented from their background and from each other in order to associate signals with specific isolated nuclei. Second, subsignals composing non-dot-like signals are detected and clustered to signals. Features are measured to the signals and a subset of these features is selected representing the signals to a multiclass classifier. Classification using a naive Bayesian classifier (NBC) or a multilayer perceptron is accomplished. When applied to a FISH image database, dot and non-dot-like signals were segmented almost perfectly and then classified with accuracy of approximately 80% by either of the classifiers.     

A new approach for approximating node deletion problems

We present a new approach for approximating node deletion problems by combining the local ratio and the greedy multicovering algorithms. For a function , our approach allows to design a 2+max_v∈V(G)logf(v) approximation algorithm for the problem of deleting a minimum number of nodes so that the degree of each node v in the remaining graph is at most f(v). This approximation ratio is shown to be asymptotically optimal. The new method is also used to design a 1+(log2)(k−1) approximation algorithm for the problem of deleting a minimum number of nodes so that the remaining graph contains no k-bicliques.     

MEG source localization using an MLP with a distributed output representation

We present a system that takes realistic magnetoencephalographic (MEG) signals and localizes a single dipole to reasonable accuracy in real time. At its heart is a multilayer perceptron (MLP) which takes the sensor measurements as inputs, uses one hidden layer, and generates as outputs the amplitudes of receptive fields holding a distributed representation of the dipole location. We trained this Soft-MLP on dipolar sources with real brain noise and converted the network's output into an explicit Cartesian coordinate representation of the dipole location using two different decoding strategies. The proposed Soft-MLPs are much more accurate than previous networks which output source locations in Cartesian coordinates. Hybrid Soft-MLP-start-LM systems, in which the Soft-MLP output initializes Levenberg-Marquardt, retained their accuracy of 0.28 cm with a decrease in computation time from 36 ms to 30 ms. We apply the Soft-MLP localizer to real MEG data separated by a blind source separation algorithm, and compare the Soft-MLP dipole locations to those of a conventional system.     

Recovery of surface bacteria from and surface sanitization of cantaloupes

Practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. Cantaloupes (Cucumis melo L. var. reticulates) were immersed in an inoculum containing Salmonella enterica serovar Poona or Pantoea agglomerans at ca. 10(4) to 10(5) CFU/ml. An efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. The method consisted of washing the entire melon with Butterfield's buffer containing 1% Tween 80 in a plastic bag placed inside a plastic pail affixed to an orbital shaker. Levels of S. enterica Poona recovered by washing the entire melon were significantly higher than those recovered by the more common laboratory method of blending the rind. P. agglomerans can be used as a non-pathogenic proxy for S. enterica Poona. A three-compartment surface sanitization method consisting of washing with an antimicrobial soap solution, scrubbing with a brush in tap water, and immersion in 150 ppm of sodium hypochlorite reduced the initial level of recoverable viable bacteria by 99.8%. When examined separately, scrubbing with a vegetable brush in tap water, washing with soap, and dipping in chlorine were found to reduce the bacterial load by 70, 80, and 90%, respectively.     

Molecular mechanisms of G protein-coupled receptor desensitization and resensitization

Beta-arrestin proteins play a dual role in regulating G protein-coupled receptor (GPCR) responsiveness by contributing to both receptor desensitization and internalization. Recently, beta-arrestins were also shown to be critical determinants for beta2-adrenergic receptor (beta2AR) resensitization. This was demonstrated by overexpressing wild-type beta-arrestins to rescue the resensitization-defect of a beta2AR (Y326A) mutant (gain of function) and overexpressing a dominant-negative beta-arrestin inhibitor of beta2AR sequestration to impair beta2AR dephosphorylation and resensitization (loss of function). Moreover, the ability of the beta2AR to resensitize in different cell types was shown to be dependent upon beta-arrestin expression levels. To further study the mechanisms underlying beta-arrestin function, green fluorescent protein was coupled to beta-arrestin2 (beta arr2GFP), thus allowing the real-time visualization of the agonist-dependent trafficking of beta-arrestin in living cells. Beta arr2GFP translocation from the cytoplasm to the plasma membrane proceeded with a time course, sensitivity and specificity that was indistinguishable from the most sensitive second messenger readout systems. Beta arr2GFP translocation was GRK-dependent and was demonstrated for 16 different ligand-activated GPCRs. Because beta-arrestin binding is a common divergent step in GPCR signalling, this assay represents a universal methodology for screening orphan receptors, GRK inhibitors and novel GPCR ligands. Moreover, beta arr2GFP provides a valuable new tool to dissect the biological function and regulation of beta-arrestin proteins.