Molecular identification of nine commercial flaffish species by polymerase chain reaction-restriction fragment length polymorphism analysis of a segment of the cytochrome b region

Commercial refrigerated or frozen flatfish fillets are sometimes mislabeled, and identification of these mislabeled products is necessary to prevent fraudulent substitution. Identification of nine commercial flatfish species (order Pleuronectiformes), Hippoglossus hippoglossus (halibut), Lepidorhombus boscii (four-spotted scaldfish), Lepidorhombus whiffiagonis (megrin), Platichthys flesus (flounder), Pleuronectes platessa (European plaice), Reinhardtius hippoglossoides (Greenland halibut), Scophthalmus maximus (turbot), Scophthalmus rhombus (brill), and Solea vulgaris (=Solea solea) (sole), was carried out on the basis of the amplification of a 486-bp segment of the mitochondrial genome (tRNA(Glu)/cytochrome b) by using the polymerase chain reaction (PCR) and universal primers. Sequences of PCR-amplified DNA from the flatfish species were used to select eight restriction enzymes (REs). The PCR products were cut with each RE, resulting in species-specific restriction fragment length polymorphism. Seven species groups could be identified by application of the single RE DdeI and six species groups by using HaeIII, HinfI, MaeI, or MboI. Different combinations of only a couple of these REs could unambiguously identify the nine flatfish species. Genetic polymorphisms of the target sequence were examined by comparison with previously published DNA sequences, and the results of this comparison confirmed the usefulness of this technique in distinguishing and genetically characterizing refrigerated or frozen pieces of these nine flatfish species.     

Dependency treelet translation: the convergence of statistical and example-based machine-translation?

We describe a novel approach to MT that combines the strengths of the two leading corpus-based approaches: Phrasal SMT and EBMT. We use a syntactically informed decoder and reordering model based on the source dependency tree, in combination with conventional SMT models to incorporate the power of phrasal SMT with the linguistic generality available in a parser. We show that this approach significantly outperforms a leading string-based Phrasal SMT decoder and an EBMT system. We present results from two radically different language pairs, and investigate the sensitivity of this approach to parse quality by using two distinct parsers and oracle experiments. We also validate our automated bleu scores with a small human evaluation.     

Control of a Robotic Wheelchair Using Recurrent Networks

This paper describes an adaptive neural control system for governing the movements of a robotic wheelchair. It presents a new model of recurrent neural network based on a RBF architecture and combining in its architecture local recurrence and synaptic connections with FIR filters. This model is used in two different control architectures to command the movements of a robotic wheelchair. The training equations and the stability conditions of the control system are obtained. Practical tests show that the results achieved using the proposed method are better than those obtained using PID controllers or other recurrent neural networks models     

Circadian rhythm of fatty acid desaturation in mouse liver

A study was made of the diurnal changes in liver microsomal desaturation of labeled stearic, linoleic and α-linolenic acids to oleic, γ-linolenic and octadeca-6,9,12,15-tetraenoic acids, respectively. C3H-S mice were used and were exposed to light-dark cycles. A circadian rhythm was observed for stearic acid desaturation, and a different one for linoleic acid. Linoleic and α-linolenic desaturation had similar responses in the day cycle. This would indicate that different mechanisms control the oxidative desaturations of the fatty acids in the 9 and 6 carbons. The fatty acid composition of the whole liver and liver microsomes also showed variations. Remarkable oscillations were observed for stearic and oleic acids. Neither the total protein synthesis nor the free fatty acid concentration in the microsomes followed a rhythm parallel to the desaturation of the studied fatty acids. The injection of cycloheximide 4 hr before measuring the desaturation modified the circadian variation of both the 9 and 6 desaturations. The modification induced by cycloheximide was considered to indicate that both variations are related to the synthesis of specific proteins but not to that of a degradative or inhibitory protein.     

Nutritional and antioxidant status of an institutionalized elderly group in Murcia (Spain)

The aging of the population is giving rise to a large number of studies aimed at improving the quality of life, considering diet as a major contributing factor in this life stage. The objectives of the present study were to evaluate the nutritional status of a group of institutionalized elderly in Murcia, Spain, and to relate the diet to anthropometric and biochemical indices, paying special attention to total antioxidant status. The dietary evaluation showed excessive intakes of proteins and lipids, and deficiencies in those of carbohydrates, dietary fiber, zinc, iodine and vitamins A, E and D. The high proportion of overweight observed were not significantly correlated to the energy intakes. Although total plasma antioxidant status was low (0.62 mM eq Trolox and 0.98 mM eq Fe(II)) compared with reference values, estimated oxidative risk in this group of elderly was low according to plasma levels of vitamins C, A & E.     

A non-lensed fiber-optic resonance-enhanced multiphoton ionization probe

We have developed a miniature fiber-optic probe with no focusing optics for in situ analysis of volatile organic compounds (VOCs). The probe uses an optical fiber to transmit a laser pulse to a vapor sample causing it to ionize adjacent to the fiber tip through a resonance-enhanced multiphoton ionization (REMPI) process. The distal end of the optical fiber is contained co-axially within 2-mm-inner-diameter stainless steel tubing that serves as an electrode. The electrode is biased at a high positive potential to collect electrons. The current generated is shown to be proportional over about two orders of magnitude to the concentration of the species ionized. Visible wavelength REMPI spectroscopy is used to determine probe sensitivities of 20 ppb (benzene) and 43 ppb (toluene). Designing the probe without focusing optics specifies an achromatic ionization region constant in size and position as the laser wavelength is scanned, which simplifies data collection and reduction. Focusing achromatic systems are discussed and the potential signal improvement is estimated.     

Simple and stereoselective synthesis of sex pheromone of processionary mothThaumetopoea pityocampa (Denis and Schiff.)

A simple and stereoselective synthesis of (Z)-13-hexadecen-11-yn-1-yl acetate, the major component of the sex pheromone ofThaumetopoea pityocampa (Denis and Schiff.) is described. The procedure essentially involves formylation of a terminal acetylene to the corresponding aldehyde followed by a stereochemically controlled Wittig reaction, which has been studied under a variety of conditions.