Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser

In the current studies, we deposited ultra-thin hydroxyapatite films on a pure titanium substrate by pulsed laser deposition, and we examined the effects of these surfaces on rat bone marrow (RBM) cells. This method allowed deposition of 500-, 2000-, and 5000-Angstrom-thick hydroxyapatite films. X-ray diffraction showed that the amorphous films recrystallized to a hydroxyapatite crystal structure after annealing. The proliferation of RBM cells was unaffected by the hydroxyapatite films, but osteocalsin and alkaline phosphatase mRNA and protein levels were elevated in cells grown on 2000- and 5000-Angstrom-thick films. These results indicate that ultra-thin hydroxyapatite films generated by pulsed laser deposition are better at promoting osteogenesis than pure titanium surfaces.     

In vivo studies on chiral inversion and amino acid conjugation of 2-[4-(3-methyl-2-thienyl)phenyl]propionic acid in rats and dogs

The relationship between chiral inversion and stereoselective amino acid conjugation of a new nonsteroidal anti-inflammatory agent, R, S-2-[4-(3-methyl-2-thienyl)phenyl]propionic acid (R,S-MTPPA) was investigated in rats and dogs. Only the S-enantiomer was found in plasma after oral administration of S-MTPPA to both species. In contrast, the R- and S-enantiomers were both detected after the dosing of R-MTPPA. In rats, the area under the curve of S-MTPPA in plasma was only 9% of that of R-MTPPA when R-MTPPA was dosed, whereas in dogs it was 2.5 times larger than that of the R-enantiomer. After administration of R-MTPPA, both enantiomers appeared in the urine. In rats, a small amount of S-enantiomer was found in the urine, whereas in the case of dogs the amount of the S-enantiomer was larger than that of the R-enantiomer. It appears that R-MTPPA is chirally inverted to S-MTPPA in both rats and dogs, although the extent of chiral inversion is greater in dogs than in rats. In dogs, the taurine conjugate was detected in plasma, urine and feces as a major metabolite after oral administration of either R- or S-MTPPA. In this case, only S-MTPPA-taurine was detected in the urine after the administration of S-MTPPA, and it was also the main component after administration of R-MTPPA.     

A simple formula for the dielectric relaxation time—temperature—pressure relationship of lubricating oils and its application to viscosity prediction

The dielectric constant and the loss factor for several lubricating oils were both measured within the frequency range of 100 Hz–1.5 MHz. Measurements were made at atmospheric pressure with varying temperature, and at fixed temperature with varying pressure. The temperature dependence of the dielectric relaxation time could be expressed by the Vogel—Fulcher—Tammann (VFT) equation. In order to express the dielectric relaxation time as a function of temperature and pressure, a pressure‐dependent term was introduced into the characteristic temperature of the VFT equation. The experimental relaxation time could then be represented by a simple formula. Moreover, high‐pressure viscosity was calculated from the dielectric relaxation data by introducing the pressure dependence of the bulk modulus. The predicted results showed fairly good agreement with the viscosity data.     

Development of In Vitro and In Vivo Evaluation Systems for Vitamin D Derivatives and Their Application to Drug Discovery

We have developed an in vitro system to easily examine the affinity for vitamin D receptor (VDR) and CYP24A1-mediated metabolism as two methods of assessing vitamin D derivatives. Vitamin D derivatives with high VDR affinity and resistance to CYP24A1-mediated metabolism could be good therapeutic agents. This system can effectively select vitamin D derivatives with these useful properties. We have also developed an in vivo system including a Cyp27b1-gene-deficient rat (a type I rickets model), a Vdr-gene-deficient rat (a type II rickets model), and a rat with a mutant Vdr (R270L) (another type II rickets model) using a genome editing method. For Cyp27b1-gene-deficient and Vdr mutant (R270L) rats, amelioration of rickets symptoms can be used as an index of the efficacy of vitamin D derivatives. Vdr-gene-deficient rats can be used to assess the activities of vitamin D derivatives specialized for actions not mediated by VDR. One of our original vitamin D derivatives, which displays high affinity VDR binding and resistance to CYP24A1-dependent metabolism, has shown good therapeutic effects in Vdr (R270L) rats, although further analysis is needed.     

Development of an ultrafast X‐ray computed tomography scanner system: Application for measurement of instantaneous void distribution of gas–liquid two‐phase flow

An ultrafast X‐ray computed tomography (CT) scanner has been developed. This scanner overcomes problems that occur in a transient or unsettled state, which make the conventional CT scanner inappropriate. To reduce the scanning time, this X‐ray CT system uses electronic switching of electron beams for X‐ray generation instead of the mechanical motion adopted by conventional CT scanners. The mechanical motion is a major obstacle to improving scanning speed. A prototype system with a scanning time of 3.6 ms was initially developed and confirmed to measure the dynamic events of two‐phase flow. However, an increased scanning speed is generally required for practical use in the thermal hydraulics research field. Therefore, an advanced type which can operate under the scanning time of 0.5 ms and can measure two‐phase flow with a velocity up to 4 to 5 m/s was developed. © 2000 Scripta Technica, Heat Trans Asian Res, 29(3): 155–165, 2000     

Formation mechanisms of paramagnetic defect centers induced by excimer lasers,gamma rays,and mechanical fracturing in amorphous SiO2

The formation mechanisms of paramagnetic defect centers in amorphous SiO₂ induced by excimer lasers, ⁶⁰Co γ rays, and mechanical fracturing were investigated. Correlation between the paramagnetic defect centers and their precursors introduced during manufacture is discussed for the cases of excimer lasers and γ rays. For the case of mechanical fracturing, formation of strained Si·O·Si bonds as well as paramagnetic defects is examined. The mechanism of laser- or γ-ray-induced paramagnetic defect centers is compared with that of fracture-induced centers. © 1998 Scripta Technica, Inc. Electr Erg Jpn, 121(3): 9–19, 1997     

High-pressure viscosity prediction of di(2-ethylhexyl) phthalate and tricresyl phosphate binary mixtures using dielectric relaxation data

Dielectric permittivity and loss in di(2-ethylhexyl) phthalate and tricresyl phosphate binary mixtures were measured over a frequency range from 100 Hz to 1.5 MHz under high pressure. The mixtures showed single dielectric relaxation. The composite plots of the dielectric loss data showed one master curve and time–temperature–pressure superposition were applicable. The prediction of high-pressure viscosity was carried out from the change of dielectric relaxation time with temperature and pressure. The predicted results showed relatively good agreement with viscosity data obtained from a falling-sphere viscometer.     

Catalytic graphitization of an amorphous carbon film under focused electron beam irradiation due to the presence of sputtered nickel metal particles

Electron beam irradiation of Ni particles (diameter: ∼500 nm) in a transmission electron microscope (TEM) formed small secondary particles, which moved randomly on an amorphous carbon film. Catalytic graphitization occurred along the path traced by the moving particles. It is generally well known that heat treatment is needed to produce catalytic graphitization of an amorphous carbon. However, we rapidly produced catalytic graphitization in the presence of sputtered Ni particles by focused electron beam irradiation in the TEM although no external heating was employed.     

A design of fully soft robot actuated by gas–liquid phase change

This study aims to investigate the application of temperature gradient between the ground and the air to the locomotion of soft robot. Soft robots attract attention due to its flexible adaptability and safety to the real environment in recent years. Although many soft robots are soft themselves, they require hard material as an external device such as air compressors and external power supplies for driving. To solve the problem, some authors reported an autonomous driving robot composed of a completely flexible material using gas generation by a chemical change of hydrogen peroxide solution. However, as the driving system utilizes a chemical reaction, its driving time is relatively short. On the contrary, the proposed robot moves without electricity and any external devices such as air compressors and an electrical power supplies. It can move just on the ground using the temperature gradient between the ground and the air. In experiments, we developed some prototypes and confirmed that continuous movement could be achieved with the proposed model.