lncRNA SNHG3 acts as oncogene in ovarian cancer through miR-139-5p and Notch1

Ovarian cancer (OC) is a common malignant tumor of the female reproductive system. Long non-coding RNAs (lncRNAs) play an important role in OC occurrence and development. Thus, the function and potential mechanism of lncRNA small nucleolar RNA host gene 3 (SNHG3) was explored in the development of OC. The expression of SNHG3, microRNA (miR)-139-5p and Notch homolog 1, translocation-associated (Drosophila) (Notch1) in OC were detected by RT-qPCR or western blot assay. In addition, CCK-8 and wound-healing assays were used to detect OVCAR3 proliferation and migration ability. The targeting relationship of miR-139-5p with SNHG3 or Notch1 was verified through luciferase reporter assay. Rescue experiments were performed to confirm whether SNHG3 could mediate OVCAR3 proliferation and migration through miR-139-5p and Notch1. In OC tissues and cell lines, the expression of SNHG3 and Notch1 were significantly increased, and the expression of miR-139-5p was significantly decreased. SNHG3 inhibition suppressed the proliferation and migration of OVCAR3 cells. Luciferase reporter experiment confirmed that miR-139-5p could target SNHG3 and Notch1. Transfection of miR-139-5p inhibitor significantly reversed the inhibitory effect of SNHG3 knockdown on OVCAR3 proliferation and migration. Moreover, SNHG3 inhibition or miR-139-5p mimic abolished the promotion of Notch1 overexpression on OVCAR3 proliferation and migration. In conclusion, SNHG3 could accelerate the proliferation and migration of OC cells by regulating miR-139-5p and Notch1.     

鼻源性疾病导致突眼症的临床分析

目的：探讨鼻源性疾病引起突眼症的原因及多发部位。方法：收集1998—2004年收治以突眼为主诉的患者30例，分析其发病临床特征、部位及疾病性质。结果：筛窦疾病是易引起突眼症的主要原因，以良性肿瘤为主。结论：对以突眼为主诉的患者，应积极排除鼻部的原因，以免延误治疗。     

ATP/GTP结合蛋白1基因在胃癌及食管癌组织中高表达

目的:应用荧光mRNA差异显示技术(DD-PCR),筛选胃癌及食管癌相关基因的异常表达。方法:收集临床胃癌组织样本,通过荧光差异显示技术(DD-PCR)获得胃癌样品中差异的片段,对这些片段进行克隆和测序。通过在GenBank中同源性检索,查找与差异片段相对应的同源基因。利用半定量PCR及定量PCR方法检验该基因在胃癌及食管癌组织中与其对应正常组织之间表达的差异。结果:通过DD-PCR获得的一个差异表达片段的序列对应于ATP/GTP结合蛋白1基因(A/GT-PBP1)。半定量PCR及荧光定量PCR技术检测结果表明,A/GTPBP1基因在胃癌及食管癌组织中的表达量高于其对应的正常组织(胃癌,P<0.01;食管癌,P<0.01)。该基因包含25个外显子,读码框长3561bp,编码1186个氨基酸,蛋白质相似性分析表明该蛋白为G蛋白家族成员。结论:A/GTPBP1在胃癌组织中异常表达,可能在胃癌发生过程中起调节作用。     

综合疗法治疗氯胺酮相关性膀胱损害疗效分析

目的探讨综合疗法治疗氯胺酮相关性膀胱损害的临床疗效。方法2008年2月至2012年12月中山大学附属第五医院和珠海市妇幼保健院收治13例氯胺酮相关性膀胱损害病例,男8例,女5例：2例可见肉眼血尿,9例镜下血尿;合并不同程度蛋白尿13例;其中1例患者孕2周;病程4～20个月;13例患者分别有9个月至6年的氯胺酮接触史,其中6例频繁混用氯胺酮、摇头丸7例间断、较长期地吸食氯胺酮。给予戒断毒品、心圳干预、口服d或M-受体阻滞药和膀胱功能训练等综合治疗。根据O’Leary—Sant间质性膀胱炎症状评分（ICSI）、问题评分（ICPI）和最大测压膀胱容量评估疗效。结果13例患者经综合治疗20d后,尿频、尿急症状逐渐改善,随着治疗期延长,症状改善越明显。治疗第20天,患者肝肾功能恢复、尿常规正常、病原微生物培养复查阴性;5例肾积水患者2例消失,2例减轻,1例变化不明显。治疗后,最大测压膀胱容量明显增加[（194±39）ml比（98±90）m1],ICSI、ICPI明显下降[（3．6±1．5）分比（13．9±3．1）分,（2．5±1．9）分比（8．9±2．6）分],差异均有统计学意义（P〈0．01）。接尿间隔时间（85±26）min,夜尿0～3次,最大尿流量（14．2±5．3）m／s。控制排尿期间,3例出现明显耻骨上区疼痛,注射回苯三酚后缓解。随访6～18个月,平均（12±3）个月,12例症状缓解明显或消失;1例复吸食者症状复发。结论及时戒毒、心理十预和膀胱功能训练等综合疗法是治疗氯胺酮相关性膀胱损害有效的方法。     

防己黄芪汤对兔肺缺血再灌注后肺水肿的保护作用

目的探讨防己黄芪汤对兔肺缺血再灌注后肺水肿的保护作用。方法建立在体兔肺缺血再灌注动物模型。防己黄芪汤组术前连续7 d灌服防己黄芪汤,行左肺缺血再灌注处理;对照组仅行左肺缺血再灌注处理;假手术组只开胸并套带不行缺血再灌注处理。切取各组左肺下叶,部分行组织学观察,部分通过测量肺湿干重的方法计算肺含水量,余肺组织检测超氧化物歧化酶(SOD)含量。最后比较3组肺血管通透性。结果缺血再灌注处理后,对照组肺含水量及伊文思兰含量分别为(89.52±0.36)%和(182.6±17.7)μg/g,明显高于假手术组的(75.57±0.22)%和(113.8±11.5)μg/g;对照组SOD含量(94.32±9.96)NU/mL,明显低于假手术组的(113.52±11.23)NU/mL(P均<0.05),肺组织损伤严重并可见严重的肺水肿。防己黄芪汤组上述指标水平分别为(81.89±0.27)%、(129.3±13.1)μg/g和(126.34±12.56)NU/mL,肺含水量及伊文思兰含量较对照组明显下降,SOD含量明显升高(P均<0.05),组织损伤轻微,肺水肿程度很轻。结论防己黄芪汤通过清除氧自由基、降低肺血管通透性等作用显著减轻了肺缺血再灌注后肺水肿的程度。     

吸痰管护套在微波聚束热疗测温导丝放置中的应用

目的探讨微波聚束热疗中应用吸痰管护套放置测温导丝的安全性和有效性。方法将121例热疗患者按病案号单双号分为对照组58例、观察组63例,对照组按常规放置测温导丝,观察组应用吸痰管护套放置测温导丝,分别观察两组导丝放置一次成功率和患者体温、不适感、直肠黏膜损伤及导丝损坏情况。结果导丝一次放置成功率对照组为82.8%、观察组100%;两组肛温检测差异无统计学意义;对照组70.7%患者有不适感、直肠黏膜损伤发生率32.8%,观察组23.8%患者有不适感、直肠黏膜损伤发生率1.6%;对照组导丝损坏5根,观察组导丝无损坏。结论热疗中应用吸痰管护套不影响测温精确度,且可降低患者不舒适感及直肠黏膜损伤发生率。     

不同发展阶段食管癌人群膳食模式及维生素A、E和β-胡萝卜素摄入量的比较

目的：探讨在由正常食管发展到食管癌的不同阶段,林州市居民膳食模式及膳食中维生素A、E和β-胡萝卜素摄入量之间的差异。方法：随机抽取林州市合涧乡的4个村,对村中40～69岁居民进行膳食调查。将资料完整且无其他消化系统疾病的人作为研究对象,根据胃镜普查及病理检验结果分为5组(正常居民173例、轻度不典型增生281例、中度不...     

MDA5 is SUMOylated by PIAS2β in the upregulation of type I interferon signaling

Retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) are cytosolic viral RNA sensors that induce type I interferon production (IFN). In this study, we found that MDA5 undergoes inducible SUMOylation by small ubiquitin-like modifier-1 (SUMO-1) in response to polyI:C stimulation. Enhanced SUMOylation of MDA5 by exogenously expressed SUMO-conjugating enzyme Ubc9 correlated with upregulation of IFN expression and repressed virus replication. Conversely, overexpression of a SUMOylation-deficient mutant of Ubc9 or knockdown of endogenous Ubc9 reduced IFN production. Furthermore, we showed that PIAS2β, a SUMOylation E3 ligase, could specifically interact with and enhance the SUMOylation of MDA5. Consequently, PIAS2β knockdown reduced the SUMOylation of MDA5 and the IFN production. Collectively, these findings suggest that SUMO-1 modification of MDA5 possibly via PIAS2β may play a role in the MDA5-mediated IFN response to viral infections.     

Regional variations in and correlates of disability-free life expectancy among older adults in China

Background  

Considerable socioeconomic and health inequalities have been reported in China. However, because of a lack of appropriate data, limited research has been conducted on variations in disability-free life expectancy (DFLE) among older adults. This study aimed to use the most up-to-date disability survey data to explore geographical variations in DFLE at age 60 in China and to identify the socioeconomic and health care factors that partially account for these variations.     

鼻咽癌患者血浆miR-24异常表达的临床意义

目的探讨miR-24在鼻咽癌患者血浆中的表达及临床意义。方法收集2007年12月~2011年6月在南方医院耳鼻喉科住
院的初诊鼻咽癌患者血浆217例,以同期住院的慢性化脓性中耳炎或慢性鼻窦炎患者血浆73例作为对照,利用荧光定量PCR的
方法进行miR-24 表达的检测并结合临床资料分析其临床意义。结果血浆miR-24 在鼻咽癌组的相对表达水平为对照组的
4.808倍,差异具有显著统计学意义（P<0.001）;血浆miR-24在不同T分期之间有统计学差异（P=0.007）,miR-24与N分期呈负
相关（P=0.028）;鼻咽癌病人血浆EBV-DNA与血浆miR-24呈负相关（P=0.048）;治疗后血浆miR-24含量较治疗前下降差异有
显著统计学意义（P=0.001）。结论血浆miR-24可作为肿瘤分子标志物用于鼻咽癌早期诊断及预后分析。