全文获取类型
收费全文 | 2126篇 |
免费 | 88篇 |
国内免费 | 79篇 |
学科分类
医药卫生 | 2293篇 |
出版年
2023年 | 10篇 |
2022年 | 10篇 |
2021年 | 26篇 |
2020年 | 22篇 |
2019年 | 22篇 |
2018年 | 20篇 |
2017年 | 30篇 |
2016年 | 25篇 |
2015年 | 24篇 |
2014年 | 35篇 |
2013年 | 55篇 |
2012年 | 75篇 |
2011年 | 76篇 |
2010年 | 62篇 |
2009年 | 73篇 |
2008年 | 59篇 |
2007年 | 112篇 |
2006年 | 60篇 |
2005年 | 77篇 |
2004年 | 61篇 |
2003年 | 69篇 |
2002年 | 68篇 |
2001年 | 88篇 |
2000年 | 69篇 |
1999年 | 75篇 |
1998年 | 80篇 |
1997年 | 80篇 |
1996年 | 62篇 |
1995年 | 72篇 |
1994年 | 45篇 |
1993年 | 40篇 |
1992年 | 40篇 |
1991年 | 31篇 |
1990年 | 33篇 |
1989年 | 49篇 |
1988年 | 52篇 |
1987年 | 36篇 |
1986年 | 46篇 |
1985年 | 60篇 |
1984年 | 29篇 |
1983年 | 27篇 |
1982年 | 28篇 |
1981年 | 19篇 |
1980年 | 28篇 |
1979年 | 14篇 |
1978年 | 16篇 |
1977年 | 20篇 |
1976年 | 27篇 |
1975年 | 15篇 |
1974年 | 9篇 |
排序方式: 共有2293条查询结果,搜索用时 78 毫秒
71.
Metabolic activity of phagocytosing granulocytes in chronic granulocytic leukemia: ultrastructural observation of a degranulation defect 总被引:4,自引:0,他引:4
Cramer E; Auclair C; Hakim J; Feliu E; Boucherot J; Troube H; Bernard JF; Bergogne E; Boivin P 《Blood》1977,50(1):93-106
The functional capacities of granulocytes in patients with chronic granulocytic leukemia are still a subject of controversy, probably due to the heterogeneity of the abnormalities observed from patient to patient. For a better definition of these abnormalities, 14 patients with untreated chronic granulocytic leukemia were studied. The patients were divided into three groups on the basis of the functional activities of their phagocytosing granulocytes. In four patients (group I), the granulocytes were normal in respect to particle ingestion, nitroblue tetrazolium (NBT)-stimulated reduction, cyanide-insensitive oxygen (O2) consumption, superoxide anion (O2-)-stimulated production, hydrogen peroxide (H2O2) production, and iodination. They also had a normal myeloperoxidase (MPO) content. In four patients (group III), the granulocytes were significantly defective in all of these activities. In the six remaining patients (group II), all the initial metabolic steps of the phagocytosing granulocytes (ingestion, NBT reduction, O2 consumption, O2-production, H2O2 production) were normal, as were the MPO content of the granulocytes, while iodination was strikingly decreased. These metabolic features suggested a degranulation defect which was observed ultrastructurally in the only patient studied among these six. The phagocytosing granulocytes of this patient did not degranulate and no deposits of MPO activity were seen in the phagosomes. 相似文献
72.
73.
TCR gamma delta bearing lymphocyte clones with lymphokine-activated killer activity against autologous leukemic cells 总被引:1,自引:0,他引:1
Activated T lymphocytes with the T-cell receptor (TCR) gamma delta (CD3+ and TCR delta 1+) exhibit strong cytotoxic activity against the standard natural killer (NK) and lymphokine-activated killer (LAK) sensitive target cells. In order to test the cytotoxic activity of gamma delta T lymphocytes against autologous leukemic cells, 84 clones of gamma delta T lymphocytes were obtained from the peripheral blood of three acute lymphoblastic leukemia (ALL) patients. Forty-four of these T-cell clones were active against an LAK-sensitive cell line and the other 40 were active against K562, an NK target cell line. In each of the three patients, cytotoxic clones against autologous leukemic cells were obtained. Among the 84 clones, ten were able to kill autologous tumor cells, including eight that lyse the LAK-sensitive target and two with NK activity. The clones were highly cytotoxic, stable, and easily expanded in large quantity. 相似文献
74.
Induction of monocyte tissue factor expression by antibodies to heparin-platelet factor 4 complexes developed in heparin-induced thrombocytopenia 总被引:5,自引:2,他引:5 下载免费PDF全文
The pathogenesis of thrombosis in heparin-induced thrombocytopenia (HIT) was studied by investigating whether antibodies to heparin-platelet factor 4 (H-PF4) induced tissue factor (TF) synthesis by monocytes. Plasma from 5 patients with HIT containing IgG to H-PF4 was incubated with peripheral blood mononuclear cells without or with purified PF4 and heparin. Significant TF-dependent procoagulant activity (PCA) expressed by monocytes, measured with a factor Xa-based chromogenic assay, was induced after incubation of each HIT plasma sample. This monocyte PCA required the presence of PF4 and was inhibited by high concentrations of heparin. Furthermore, purified HIT IgG added to whole blood with PF4 and heparin also provoked significant synthesis of TF mRNA by monocytes, demonstrated by RT-PCR, and this effect was not observed with normal IgG. These findings strongly support the hypothesis that antibodies to PF4 developed in HIT trigger the production of tissue factor by monocytes, and this effect could account in vivo for hypercoagulability and thrombotic complications in affected patients. 相似文献
75.
76.
77.
78.
Alain Spatz Dirk Ruiter Thomas Hardmeier Nathalie Renard Janine Wechsler Christiane Bailly Marie-Franoise Avril Herry Kwee Boris C. Bastian Catherine Hill Christian De Potter Michel Prade 《International journal of cancer. Journal international du cancer》1996,68(3):317-324
Melanoma in children is rare. Nevertheless, it is imperative that clinicians be aware that melanoma does occur in childhood. Yet there is very little information available on the clinico-pathologic variations, and the prognostic parameters of melanoma in children. This report presents the results of a multicenter study of 102 lesions originally diagnosed as cutaneous melanoma, conducted among 5 Western European countries and collected during the period 1961–1994. Criteria for inclusion in the study included: (1) diagnosis of cutaneous melanoma; (2) age up to 16 years at diagnosis; and (3) availability of representative microscopic slides. On the basis of the histologic review only, 60 lesions were confirmed as melanoma, and 42 lesions initially diagnosed as melanoma were reclassified as nevi; 31 of the latter contained a predominance of spindle cells. The only significant parameter associated with the development of metastases or fatal outcome was thickness of more than 2.00 mm. The 5-year survival rate observed in this study was 84%. Based on these findings we conclude that considerable over-diagnosis of melanomas in children occurs. In order, therefore, to give consistent epidemiological data on melanomas in children and to improve proper recognition of their diagnostic features, both by clinicians and by pathologists, we propose to set up a central registry of melanomas in children in Europe, under the auspices of the European Organization for Research and Treatment of Cancer. © 1996 Wiley-Liss, Inc. 相似文献
79.
Geert Molenberghs Christel Faes Johan Verbeeck Patrick Deboosere Steven Abrams Lander Willem Jan Aerts Heidi Theeten Brecht Devleesschauwer Natalia Bustos Sierra Franoise Renard Sereina Herzog Patrick Lusyne Johan Van der Heyden Herman Van Oyen Pierre Van Damme Niel Hens 《Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin》2022,27(7)
BackgroundCOVID-19 mortality, excess mortality, deaths per million population (DPM), infection fatality ratio (IFR) and case fatality ratio (CFR) are reported and compared for many countries globally. These measures may appear objective, however, they should be interpreted with caution.AimWe examined reported COVID-19-related mortality in Belgium from 9 March 2020 to 28 June 2020, placing it against the background of excess mortality and compared the DPM and IFR between countries and within subgroups.MethodsThe relation between COVID-19-related mortality and excess mortality was evaluated by comparing COVID-19 mortality and the difference between observed and weekly average predictions of all-cause mortality. DPM were evaluated using demographic data of the Belgian population. The number of infections was estimated by a stochastic compartmental model. The IFR was estimated using a delay distribution between infection and death.ResultsIn the study period, 9,621 COVID-19-related deaths were reported, which is close to the excess mortality estimated using weekly averages (8,985 deaths). This translates to 837 DPM and an IFR of 1.5% in the general population. Both DPM and IFR increase with age and are substantially larger in the nursing home population.DiscussionDuring the first pandemic wave, Belgium had no discrepancy between COVID-19-related mortality and excess mortality. In light of this close agreement, it is useful to consider the DPM and IFR, which are both age, sex, and nursing home population-dependent. Comparison of COVID-19 mortality between countries should rather be based on excess mortality than on COVID-19-related mortality. 相似文献
80.
Afifi K. Bellanger G. Buyck P. J. Zuurbier S. M. Garcia-Esperon C. Barboza M. A. Costa P. Escudero I. Renard D. Lemmens R. Hinteregger N. Fazekas F. Jimenez-Conde J. Giralt-Steinhauer E. Hiltunen S. Arauz A. Pezzini A. Montaner J. Putaala J. Weimar C. Schlamann Marc Gattringer T. Tatlisumak T. Coutinho J. M. Demaerel P. Thijs V. 《Journal of neurology》2020,267(11):3299-3300
Journal of Neurology - The original version of this article unfortunately contained mistakes. The correct information is given below. 相似文献