小胶质细胞极化中的糖代谢重编程研究进展

静息态或M2表型的小胶质细胞主要由葡萄糖通过氧化磷酸化为细胞提供能量,病理条件下的M1促炎型小胶质细胞即使在氧气充足条件下,葡萄糖也会被优先用于糖酵解生成大量乳酸,三羧酸循环受到抑制。蛋白激酶 B（Akt）及AMP依赖的蛋白激酶（AMPK）/哺乳动物雷帕霉素靶蛋白（mTOR）/缺氧诱导因子-1α（HIF-1α）信号通路在调节糖酵解与炎症反应过程中发挥重要作用。小胶质细胞中糖酵解与炎症存在制约关系,抑制糖代谢重编程能够干预小胶质细胞 M1炎症表型极化状态,可以作为抑制神经炎症的药物作用靶点,为退行性疾病、脑血管疾病等相关药物的研发提供新思路。     

延续性护理干预对高血压患者生活质量的影响

目的 探讨系统的护理干预对高血压患者血压控制效果和生活质量的影响.方法 选择2009年1～6月接受治疗的高血压患者108例,随机分为研究组和对照组,每组各54例.对照组采用常规的高血压用药治疗和一般性的护理,研究组在此基础上,进行饮食、休息、运动、心理和疾病的认知等方面延续性系统的护理干预,比较实施前、后动态血压变化和生活质量的变化.结果 干预后,研究组血压控制效果及生活质量得分均优于对照组（P ＜ 0.05）;对照组干预后血压水平、生活质量的得分与干预前比较,差异无统计学意义（P ＞ 0.05）;研究组干预后生活质量得分高于干预前（P ＜ 0.05）,高血压程度减轻（P ＜ 0.05）.结论 系统的护理干预可有效降低高血压,提高患者的生活质量.     

黄蛭口服液质量标准研究

目的:建立黄蛭口服液的质量标准。方法:采用薄层色谱(TLC)法对黄蛭口服液中的大黄、水蛭、牛蒡子进行定性鉴别,以凯氏定氮法测定制剂中总氮含量,以高效液相色谱法对其主药大黄中的大黄酚进行含量测定。结果:大黄、水蛭、牛蒡子的TLC中,在与对照药材色谱相应的位置上,显相同颜色的斑点。大黄酚的检测浓度在1.696～8.480μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为99.60%,RSD=1.37%(n=9)。结论:所建标准可用于黄蛭口服液的质量控制。     

石上柏化学成分、药理作用及临床应用研究进展

目的促进广东地产药材石上柏的研究及合理开发利用。方法查阅近几年来相关文献,从化学成分、药理作用及临床应用等方面对其研究进展进行综述。结果石上柏在抗肿瘤作用方面的研究取得了一定进展,但研究深度和广度尚不够;其药效物质基础揭示还不够清楚。结论石上柏在治疗鼻咽癌、绒毛膜上皮癌、肺癌及消化道癌等多种肿瘤方面均取得了很好的疗效,具有较好的开发应用前景。     

干细胞因子与白介素-3联合对小鼠骨髓细胞体外扩增作用及其造血重建的影响

采用集落培养的方法研究了干细胞因子（ Ｓ Ｃ Ｆ） 联合白介素 ３（ Ｉ Ｌ ３） 对小鼠５ 氟尿嘧啶（５ Ｆ Ｕ ） 处理３ ｄ 的骨髓细胞（ｄ３ ５ Ｆ Ｕ Ｂ Ｍ Ｃ） 的体外扩增作用及其对致死量照射小鼠造血重建功能的影响。 Ｓ Ｃ Ｆ 单独应用使体系中粒 巨噬细胞集落形成单位（ Ｇ Ｍ Ｃ Ｆ Ｕ） 和高增殖潜能集落形成单位（ Ｈ Ｐ Ｐ Ｃ Ｆ Ｕ） 分别增加了（２４ ．２ ±６ ．１） 和（１ ．５ ±０ ．３） 倍（ Ｐ＜ ０ ．０５） 。 Ｓ Ｃ Ｆ 与 Ｉ Ｌ ３ 联合应用时 Ｇ Ｍ Ｃ Ｆ Ｕ 和 Ｈ Ｐ Ｐ Ｃ Ｆ Ｕ 分别是新鲜ｄ３ ５ Ｆ Ｕ Ｂ Ｍ Ｃ 的（３０ ．５ ±９ ．５） 和（１３ ．６ ±４ ．５）倍（ 与单用 Ｓ Ｃ Ｆ 组比较 Ｐ 分别大于和小于０ ．０５） 。小鼠骨髓移植实验结果表明： Ｓ Ｃ Ｆ、 Ｓ Ｃ Ｆ＋ Ｉ Ｌ ３ 体外扩增的ｄ３ ５ Ｆ Ｕ Ｂ Ｍ Ｃ 移植给经致死量照射的受体小鼠后,外周血细胞计数恢复时间比输新鲜ｄ３ ５ Ｆ Ｕ Ｂ Ｍ Ｃ 组缩短１ ～５ ｄ , Ｓ Ｃ Ｆ＋ Ｉ Ｌ ３组不仅血细胞计数恢复最快,并使移植所需的骨髓细胞数减少９０ ％ 以上。结果提示： Ｓ Ｃ Ｆ 单用对骨髓细胞有扩增作用,并且与 Ｉ Ｌ ３ 有协同作用;体外扩增的骨髓?     

Electrochemical Energy Storage Properties of High-Porosity Foamed Cement

Foamed porous cement materials were fabricated with H₂O₂ as foaming agent. The effect of H₂O₂ dosage on the multifunctional performance is analyzed. The result shows that the obtained specimen with 0.6% H₂O₂ of the ordinary Portland cement mass (PC0.6) has appropriate porosity, leading to outstanding multifunctional property. The ionic conductivity is 29.07 mS cm⁻¹ and the compressive strength is 19.6 MPa. Furthermore, the electrochemical energy storage performance is studied in novel ways. The PC0.6 also shows the highest areal capacitance of 178.28 mF cm⁻² and remarkable cycle stability with 90.67% of initial capacitance after 2000 cycles at a current density of 0.1 mA cm⁻². The superior electrochemical energy storage property may be attributed to the high porosity of foamed cement, which enlarges the contact area with the electrode and provides a rich ion transport channel. This report on cement–matrix materials is of great significance for large scale civil engineering application.     

紫外分光光度法测定黑面神药材中总三萜类成分含量

目的：运用紫外分光光度法测定黑面神药材中总三萜类成分的含量,从而为有效控制药材质量提供依据。方法：以药材中主要成分三萜类的熊果酸为考察指标,以5％香草醛一冰乙酸及高氯酸为显色剂,采用紫外分光光度法在547nm波长处测定样品的吸光度。结果：总三萜类成分在8．28～49．68μg范围内呈良好线性关系,其回归方程为Y=10．507X＋0．0077,r=0．9995,黑面神中总三萜类成分的含量约为3．89％。结论：紫外分光光度法适合黑面神药材中总三萜类成分的含量测定,该方法操作简便,结果稳定,重现性好。     

Silver nanoparticles reduced the invasiveness of redroot pigweed

The differences in the growth performance between invasive species and native species might paly a key role in the effective invasion. The extensive use of silver nanoparticles (AgNPs) has created the concern of their release into environment. Thus, the possible effects of AgNPs on the growth performance of invading agents are critical to better illustrate the underlying mechanisms for effective invasion. This study aimed to assess the impacts of AgNPs with different concentrations [200 and 400 mg kg (soil)⁻¹] and particle sizes (30 and 70 nm) on the growth performance and competitive ability of well known invasive Amaranthus retroflexus L. (redroot pigweed) and native A. tricolor L. (red amaranth). It was observed that the growth characteristics and supporting ability of redroot pigweed were significantly lower than those of amaranth. Results of the relative competitive intensity index and the relative dominance index also revealed that redroot pigweed exhibited lower competitive ability compared to red amaranth, especially under AgNPs. It can be assumed that the poor growth performance and competitive ability of redroot pigweed might prevent its invasiveness under AgNPs. The supporting ability, leaf photosynthetic area, leaf growing ability, leaf resource use efficiency and acquisition capability, and growth competitiveness of the two plant species were found to be significantly reduced under AgNPs. AgNPs with 30 nm at 400 mg kg (soil)⁻¹ triggered more toxicity on the supporting ability and growth competitiveness of the two plant species than AgNPs with 30 nm at 200 mg kg (soil)⁻¹. In addition, AgNPs with 30 nm imparted high toxicity on the leaf growing ability of red amaranth than AgNPs with 70 nm. However, the particle size of AgNPs did not address significant effects on the growth performance of redroot pigweed. Ag⁺ solution exhibited stronger toxicity on the supporting ability and leaf growing ability of the two plant species than AgNPs.

     

骨科洗剂2号方中桂枝、独活、威灵仙的薄层色谱鉴别

目的:建立骨科洗剂2号方中桂枝、独活、威灵仙的薄层鉴别方法。方法:采用《中国药典》一部附属VIB中薄层色谱法鉴别骨科洗剂2号中的桂枝、独活、威灵仙。结果:骨科洗剂2号中的桂枝、独活、威灵仙和对照药材薄层色谱主斑点颜色与位置一致;且斑点集中,分离度好。结论:所建立的方法简便、灵敏、专属性强,可作为骨科洗剂2号的质量控制方法之一。