水通道蛋白1在急性坏死性胰腺炎大鼠胰腺组织的表达及其意义

Objective To study the expression of aquaporin 1 ( AQP1 ) in pancreas and its pathogenic role in rats with experimental acute necrotizing pancreatitis (ANP). Methods Forty-eight male Sprague-Dawley rats were randomly divided into two groups : control group ( n = 24 ) and ANP group ( n = 24). Six rats in each group were sacrificed at 3,6,12 and 18 h after induction of experimental models. Quantity of ascites and levels of serum amylases were measured at each time point. Serum AQPI was determined by ELISA. Pathological changes of pancreatic tissues were examined. Capillary permeability in pancreatic tissues was tested by Evans Blue (EB) extravasation method. AQPI expression in pancreatic tissues was detected by immunohistochemieal staining, Western blot and fluorescence quantitative polymerase chain reaction (FQ-PCR). Results (1) Serum amylase level at 3,6,12, and 18 h in control group was (1308±759) ,(1077±508), (1325±761),(1328±762) U/L,and that in ANP group was (9102± 2199), (8799±1634), (9398±1473), (9484±862) U/L respectively. The concentration of EB in pancreatic tissues at each time point in control group was (205.61±32.99), (141.46±27.18 ), (96.94± 26.79), (61.43±24.82) mg/L, and that in ANP group was ( 273.59±23.47 ), ( 253.51±31.68 ), (221.15±73.68 ), (185.28±42.35) ,respectively. There was significandy difference between two groups. Serum AQP1 level in control group at each point was ( 74.08±11.80), (78.49±9.06 ), (75.77± 7.37 ), ( 72.75±13.87 ) mg/L, and that in AN P group was (73.29±9.61 ), ( 62.85±7.28 ), (62.07± 4.39 ), (46.33±11.91 ) mg/L, respectively ( P < 0.01 ). ( 2 ) Protein expression of AQPI in pancreatic tissues detected by immunohistochemical staining in control group at each time point was 114.13±7.92, 122.39±7.99,145.98±6.48,113.98±6.48, and that in ANP group was 80.07±14.89,110.54± 4.45,103.77±10.48,99.18±6.95;Protein expression of AQP1 in pancreatic tissues detected by Western blot in control group at each time point was 1.19±0.33,1.02±0.25,0.90±0.33,1.06±0.20,and that in ANP group was 0.83±0.11,0.96±0.21,0. 58±0.28,0.72±0.14, respectively ( P < 0.05 ). ( 3 ) The mRNA level of AQP1 gene expressed in pancreas in control group at each time point was 0.91±0.22,1.01±0.83,0.48±0.23,0.61±0.51,and that in ANP group was 2.13±0.63,2.02±1.40, 2.07±0.86,2.49±2.47,respectively (P<0.01).Condusion The expression of AQP1 wasdown-reg-ulated in pancreas in rats with ANP,which might could play an important role in the pathogenesis of capil-lary leak syndrome.     

天然维生素E微囊的制备及含量测定

目的:探讨天然维生素E微囊的制备工艺。方法:根据天然维生素E易氧化且为油状液体服用不便的特性,用复凝聚法制成微囊。并以正己烷作溶剂,用研磨-超声波振荡法得微囊提取物,按2000年版《中国药典》维生素E含量测定项下方法,采用气相色谱法测定其含量。结果:经计算微囊中天然维生素E的含量为22.9%,包油率为76.5%。结论:采用复凝聚法能成功地将天然维生素E由油状液体转变为微囊,方法可行,提高了天然维生素E利用率,能为其制剂提供较高的质量及稳定性。     

脱落细胞冰冻切片技术

脱落细胞冰冻切片技术湖南省肿瘤医院陈森林，何执鼎，吴扬，蔡杰各种脱落细胞的检查，对良恶性肿瘤的鉴别颇为重要。但由于常规细胞涂片细胞分散，范围大，常影响诊断结果，更不适宜于免疫组化和原位分子杂交技术。为此，许多作者用塑料包埋切片，明胶包埋切片及石蜡双重...     

248例心悸患者24h动态心电图分析

目的探讨心悸患者的病因,研究心悸与心律失常及其与心血管疾病之间的关系。方法将248例心悸患者分为器质性与非器质性两组,均进行24h动态心电图(DCG)监测分析。结果 248例心悸患者DCG检查共发现各种心律失常227例,检出率为91．53％。室性心律失常所致的心悸显著高于房性心律失常。频发室性期前收缩与短阵房性心动过速、心房纤颤多发生于器质性心脏病,心悸发作时器质性组与非器质性组出现心律失常的几率接近。结论心律失常和心悸的发作有直接关系。     

有限采样策略应用于中国器官移植患者霉酚酸暴露量估算的研究进展

霉酚酸(mycophenolic acid,MPA)是一种抗代谢免疫抑制药,广泛应用于实体器官移植术后,其具有治疗窗窄、药动学个体差异大等特点,常常需要治疗药物监测(therapeutic drug monitoring,TDM)测定药-时曲线下面积(area under the concentration-time ...     

益肾合剂治疗早期糖尿病肾病的临床研究

目的 :观察益肾合剂治疗早期糖尿病肾病 (DN )的临床疗效。方法 :6 4例患者随机分为治疗组 32例和对照组 32例。对照组给予糖尿病教育、基础治疗 ,治疗组加服益肾合剂治疗。12周为一疗程。结果 :两组均能较好地降低症状积分、空腹血糖 (FBG)及 2 4小时尿白蛋白排泄率 (UAER) ,治疗组还能升高超氧化物歧化酶 (SOD)活性、降低丙二醛 (MDA) ,并能显著减少UAER ,对照组SOD、MDA的改善不明显 ,两组差异有显著性意义 (P <0 0 5或P <0 0 1) ,治疗组疗效明显优于对照组 (P <0 0 5或P <0 0 1)。结论 :益肾合剂对早期DN具有肯定疗效     

中药在实验性肺间质纤维化中的研究进展

肺间质纤维化又称为弥漫性肺间质疾病,其主要病理表现为弥漫性肺泡炎、肺泡单位结构紊乱及肺纤维化[1].其病因非常复杂,发病机制尚不十分明确,临床上缺乏有效的治疗手段.目前对肺间质纤维化的治疗一般局限于非特异性抗炎、免疫抑制剂及糖皮质激素等西医治疗,疗效不能令人满意,且往往有较大的副作用.目前尚无药改变或逆转肺间质纤维化的病理炎症过程的报道.     

肺泡灌洗术与纤维支气管镜在慢阻肺并肺不张诊疗中的应用

目的:探讨肺泡灌洗术与纤维支气管镜在慢阻肺并肺不张诊疗中的应用效果.方法:选择本院收治的50例慢阻肺并肺不张患者(2013年1月—2019年12月治疗)作为观察对象,根据其采用不同的诊断方法将其分为参照组与研究组,每组各25例,参照组采用纤维支气管镜诊断,研究组采用肺泡灌洗术联合纤维支气管镜诊断.比较两组患者检出率、肺...     

我院推广成份输血工作的几项举措

由于成份血液较全血有更多的优点，卫生部已将成份血使用率列人医院分级管理标准，实行医院评审一票否决。我院在创“三甲”期间，为推广成份输血，制定了一系列措施，现我院已通过三级甲等医院的评审。以下是我院推广成份输血工作的主要举措。1组建医院输血管理委员会医院输血管理委员会由业务院长、医教科科长、血液科资历较深的教授以及血库负责人组成，负责对医院输血事务进行管理。2加强宣传教育由于旧输血观念的影响，许多医生认为输全血好，自然、安全，这是一种错误的观念，要改变这种观念，首先是要开展对成份输血的宣传教育，提高…