甲亢性周期麻痹23例分析

目的：探讨甲亢性周期性麻痹（TPP）的临床特点。方法：对23例TPP进行回顾性分析和总结。结果：TPP占同期住院甲亢患者的6.2%,男女比例22∶1,21-40岁为发病高峰年龄,肌无力发作时23例血钾均低于正常。结论：TPP以肌无力伴低钾血症为其临床特点,及早诊治是关键。TPP患者的治疗应在补钾的同时加用抗甲状腺药物,可减少复发。对重度TPP进行心电监护、补钾、抗心律失常和呼吸机辅助呼吸是抢救成功的重要措施。     

宫腔镜引导疏通输卵管治疗阻塞性不孕

目的在宫腔镜引导下疏通输卵管治疗阻塞性不孕。方法选择经子宫输卵管造影证实不同程度的输卵管阻塞120例，于月经干净3-7d进行输卵管疏通术治疗（地塞米松注射剂5mg，庆大霉素注射剂8万U，糜蛋白酶注射剂4000U）。结果单侧输卵管治疗有效率83．9％，有改善16．1％。双侧输卵管阻塞治疗有效率51．4％，有改善18．9％，无效29．7％。结论官腔镜引导下能准确判断左右两侧输卵管开口的位置及治疗情况，提高了一次疏通命中率及治愈率，是一种微创、安全、有效的治疗方法。     

低蛋白饮食对环孢素A肾病大鼠肾间质纤维化的作用

Objective To investigate whether low-protein diet has protective effect on the progression of renal interstitial fibrosis in rats with cyclosporine A (CsA)-induced nephropathy. Methods Eighteen male Sprague-Dawley rats were randomly divided into three groups (6 rats in each group). The rats in control group (C group) received common diet; in model group (M group) low-salt diet; in intervention group (Ⅰ group) low-salt and low-protein diet. After diet adaptation period of one week, the rats in C group received subcutaneous injection of olive oil 1 mg/kg daily for 5 weeks, while M group and Ⅰ group subcutaneous injection of CsA (diluted into 25 g/L with olive oil) 1 ml/kg for 5 weeks. All the rats were sacrificed at the end of the 5th week. The food-intake and body weight were measured daily. The creatinine clearance (Ccr) was examined before rats were sacrificed. The semi-quantitative pathological analysis on kidney sections was performed. The mRNA and protein expression of transforming growth factor-β1 (TGF-βI) and type Ⅰ collagen (Col Ⅰ) in kidney tissue was determined with real time PCR and immunohistochemical staining, respectively. Results The food-intake and body weight of rats in M and I groups were significantly lower than those in C group (P<0.05). Compared with C group, the Ccr levels in M and Ⅰ groups were significantly reduced [(0.65±0.15) ml/min, (0.40+0.13) ml/min vs (1.55±0.29) ml/min, P<0.05], the relative fibrosis areas of kidney interstitium in M and I groups were significantly increased (3.60%±0.46%, 3.26%±0.75% vs 0.44%±0.24%, P<0.05), the mRNA and protein expression of TGF-β1 in M and I group was significantly up-regulated (by 2.6 and 3.1 times in mRNA and by 1.5 and 1.6 times in protein, respectively, P<0.05), and the mRNA and protein expression of Col Ⅰ in M and I groups was also significantly up-regulated (by 3.0 and 3.5 times in mRNA and by 2.3 and 2.1 times in protein, respectively, P<0.05). There were no significant differences between M and I groups in every parameters above-mentioned except the rat body weight and Ccr. Both the body weight and Ccr in Ⅰ group were significantly lower than those in M group (P<0.05). Compared with C group, the urine osmotic pressure in M group and in I group were deceased (for M group, P>0.05; for I group, P<0.05). Compared with C group, the serum cholesterol levels in M and I groups were significantly increased (P<0.05), and the serum phosphorus level in I group was significantly decreased (P<0.05). The levels of serum albumin and serum calcium of all three groups had no statistical differences (P>0.05). Conclusion Low-protein diet has no renoprutective effects on the rat model of cyclosporin A nephropathy, on the contrary, may induce body weight loss.     

子宫颈环型电切除治疗宫颈病变78例分析

目的探讨宫颈环型电切术(LEEP)治疗宫颈病变的可行性。方法对门诊78例宫颈肥大、糜烂、赘生物、囊肿患者进行LEEP治疗,于术后2个月观察疗效。结果术后2个月治愈77例(98.7%),显效1例(1.3%)。结论LEEP对宫颈病变是一种快速、安全、微创、有效的治疗方法。     

丹参注射液对自发性高血压大鼠左室肥厚的影响

目的：探讨丹参对自发性高血压大鼠（SHR）左室肥厚的影响。方法：18只引种繁殖的雄性SHR随机分成三组，每组6只。对照1组育至第8周处死；丹参组从第8周给药，丹参粉针剂1g／（kg.d），腹腔注射，用药10周处死；对照2组从第8周开始用相同容量蒸馏水腹腔注射，第18周处死。测量各组大鼠尾动脉收缩压（SBP）及左心室重量指数（LVMI），应用HE、VG染色，结合计算机图像分析技术，检测心肌细胞的直径（TDM）和面积（CA）、心肌组织胶原体积比例（CVF）、血管周围胶原面积与管腔面积比例（PVCA）。三组间各指标进行显著性检验。结果：与对照1组比较，对照2组的SBP、LVMI、TDM、CA、CVF、PVCA显著增加；丹参组除SBP升高外，余指标与对照1组比较，无统计学意义；与对照2组比较差异有显著性意义，P〈0.05。结论：丹参注射液能预防自发性高血压大鼠左室肥厚的形成。     

深静脉血栓形成患者凝血因子ⅩⅢ Val34Leu基因多态性检测

目的:探讨凝血因子ⅩⅢ(FⅩⅢ)Val34Leu基因多态性与中国人群深静脉血栓形成(DVT)发病的相关性.方法:利用聚合酶链反应和限制性片段长度多态性(PCR-RFLP)方法,检测了103例DVT患者与106例正常对照的FⅩⅢVal34Leu基因多态,并加以对照分析.结果:103例DVT患者FⅩⅢVa134Leu基因型均为野生型V/V,未见突变类型;106例对照组中发现1例突变杂合子V/L.2组基因型频率、等位基因频率相互比较,差异均无统计学意义(P＞0.05).结论:FⅩⅢVal34Leu对中国人群DVT有保护作用的可能性极小.     

“秘精灵”治疗遗精，早泄376例临床观察

目的 研究观察秘精灵，早泄的临床效果。方法 对172例遗精患者，204例早泄患者全部使用秘精灵浓缩丸行治疗。结果 遗精患者治疗有效率达92．5％，早泄患者有效率达94．6％，总效率达93．5％。结论 秘精灵浓缩丸是有益肾生精，固涩止遗之功效，不以补盖肾精肾气，固摄生精，泻、固涩精通、对男子遗精，早泄有较好疗效，值得临床推广应用。     

头痛、呕吐、四肢抽搐、行走不稳

患者女性，59岁，因进行性头痛、呕吐1个月，四肢抽搐1次于2002年11月23日入院。患者于2002年10月22日起无明显诱因出现后枕及后颈部疼痛，呈阵发性，头痛剧烈时伴呕吐胃内容物，头痛可自行缓解或服用“散利痛”后缓解。2002年11月6日因着凉而出现“感冒”症状，头痛加重且持续时间长，每日发作的次数增多，可达4～5次。     

管碟法测定Nisin效价

对管碟法测定Nisin效价的条件进行了研究,考察了几个参数的影响,得出了Nisin测定的最佳条件:90mm培养皿中培养基加量为15mL,Na2HPO4·12H2O质量浓度1g/dL,菌悬液浓度109CFU/mL,琼脂质量浓度1g/dL,培养基pH值7.0,牛津杯中样品加液量100μL.在此条件下,Nisin效价在5～100IU/mL,其对数值与抑菌圈直径有较好的线性关系.